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AB277434

Human Cathepsin C ELISA Kit

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(1 Publication)

Human Cathepsin C ELISA Kit is a Sandwich ELISA for the measurement of Human Cathepsin C in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

CPPI, CTSC, Dipeptidyl peptidase 1, Cathepsin C, Cathepsin J, Dipeptidyl peptidase I, Dipeptidyl transferase, DPP-I, DPPI

1 Images
ELISA - Human Cathepsin C ELISA Kit (AB277434)
  • ELISA

Supplier Data

ELISA - Human Cathepsin C ELISA Kit (AB277434)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2 ng/mL

Range

2.048 - 500 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Cathepsin C ELISA Kit (ab277434) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Cathepsin C in serum, plasma (collect plasma using citrate and heparin as an anticoagulant. EDTA is not recommended as anticoagulants for use in this assay) and cell culture supernatants.

This assay employs an antibody specific for Human Cathepsin C coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Cathepsin C present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Cathepsin C antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Cathepsin C bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "73 - 95 %", "average": "= 80.54" }, { "sample": "Cell culture media", "range": "124 - 150 %", "average": "= 135.7" }, { "sample": "Plasma", "range": "68 - 80 %", "average": "= 71.93" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277434-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB277434-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277434-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277434-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Cathepsin C coated Microplate", "number":"AB277434-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin Concentrate", "number":"AB277434-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human Cathepsin C Antibody", "number":"AB277434-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Cathepsin C standard protein (Lyophilized)", "number":"AB277434-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277434-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin C also known as dipeptidyl peptidase I is a lysosomal cysteine protease with a molecular mass of approximately 51 kDa. It functions mechanically by cleaving off dipeptides from the N-terminal end of proteins and peptides. Cathepsin C is widely expressed in various tissues with higher levels in immune cells like neutrophils and macrophages. This enzyme activates serine proteases from their inactive zymogen forms which is important for immune responses.
Biological function summary

The enzyme contributes significantly to the immune system. It forms part of a multi-enzyme complex that processes proenzymes such as granzymes and cathepsin G into their active forms. Through these actions Cathepsin C regulates cellular activities linked to inflammation and apoptosis. The proteolytic activity it provides is critical for the functioning of immune-related cells facilitating defense mechanisms against pathogens.

Pathways

Cathepsin C plays roles in the inflammatory and immune response pathways. It connects to the granzyme and lysosomal pathways important in processing and regulating granzyme activities critical for cytotoxic T lymphocyte functions. Cathepsin C maintains connections with proteins like granzymes and cathepsin G each involved in mediating immune responses and cellular death.

Cathepsin C is associated with conditions such as Papillon-Lefèvre Syndrome and aggressive periodontitis. These disorders arise due to disruptions in immune protection resulting from mutations in the Cathepsin C gene. The enzyme's abnormal activity in these diseases highlights its connection with proteins such as neutrophil elastase which play critical roles in tissue degradation processes leading to periodontal disease.
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Product protocols

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Target data

Thiol protease (PubMed : 1586157). Has dipeptidylpeptidase activity (PubMed : 1586157). Active against a broad range of dipeptide substrates composed of both polar and hydrophobic amino acids (PubMed : 1586157). Proline cannot occupy the P1 position and arginine cannot occupy the P2 position of the substrate (PubMed : 1586157). Can act as both an exopeptidase and endopeptidase (PubMed : 1586157). Activates serine proteases such as elastase, cathepsin G and granzymes A and B (PubMed : 8428921).
See full target information CTSC
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

ERJ open research 11: PubMed40391061

2025

Exploring the roles of airway dipeptidyl peptidase 1 in obstructive airway disease.

Applications

Unspecified application

Species

Unspecified reactive species

Naoya Tanabe,Hisako Matsumoto,Mariko Kogo,Chie Morimoto,Natsuko Nomura,Yusuke Hayashi,Ryo Sakamoto,Tsuyoshi Oguma,Tadao Nagasaki,Hironobu Sunadome,Atsuyasu Sato,Susumu Sato,Kai Ohashi,Takamitsu Tsukahara,Toyohiro Hirai
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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