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AB119586

Human Cathepsin D ELISA Kit

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(10 Publications)

Human Cathepsin D ELISA Kit is a sandwich ELISA designed to quantify Human Cathepsin D with a sensitivity of 10 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 156 - 10000 pg/mL

View Alternative Names

CPSD, CTSD, Cathepsin D

2 Images
Sandwich ELISA - Human Cathepsin D ELISA Kit (AB119586)
  • sELISA

Lab

Sandwich ELISA - Human Cathepsin D ELISA Kit (AB119586)

Cathepsin D measured in biological fluids and cell culture medium with background signal subtracted (duplicates +/- SD). We recommend to test the human plasma, serum and urine samples in the range of 1 : 20-1 : 500, the saliva in the range of 1 : 100-1 : 1000 and the cell culture supernatants 1 : 1-1 : 1000.

Sandwich ELISA - Human Cathepsin D ELISA Kit (AB119586)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cathepsin D ELISA Kit (AB119586)

Representative Standard Curve using ab119586.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

156 - 10000 pg/mL

Assay Platform

Microplate

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Reactivity data

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Product details

Human Cathepsin D ELISA Kit ab119586 is a sandwich ELISA to measure Human Cathepsin D in serum, plasma, cell culture supernatant, tissue extracts, other biological fluids with a sensitivity of 10 pg/ml.

How the assay works

A Cathepsin D specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Cathepsin D is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Cathepsin D amount of sample captured in plate.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human Cathepsin D ELISA Kit ab119586 protocol summary

1. Add standard or sample to each well used. Incubate 37C
2. Add prepared biotin antibody to each well. Incubate at 37C
3. Add prepared Avidin-BiotinPeroxidase Complex (ABC). Incubate at 37C
4. Add TMB to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450 nm
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin D also known as CTSD is a protein with a mass of approximately 45 kDa. It functions as an aspartyl protease and is expressed in lysosomes across various tissues including the liver and kidneys. This enzyme acts by cleaving peptide bonds within proteins which is essential for protein degradation and turnover. Cathepsin D exists as precursor forms that become activated in the acidic environment of the lysosome. It plays a critical role in normal cellular processes by maintaining protein homeostasis.
Biological function summary

The enzymatic activity of Cathepsin D is important for cellular maintenance and apoptosis. This protease does not act within larger protein complexes but contributes to the degradation of extracellular and intracellular proteins. It mediates processes like antigen processing where it deconstructs proteins into peptides that are presented on major histocompatibility complex (MHC) molecules. ELISA tests can quantify its expression levels sometimes termed as CTSD activity in various biological samples offering insights into its role within cellular environments.

Pathways

Cathepsin D involvement includes the lysosomal degradation pathway and the apoptotic signaling pathway. In the lysosomal degradation pathway Cathepsin D breaks down proteins and peptides a process important for cellular recycling and energy release. It interacts with other lysosomal enzymes such as Cathepsin B in this pathway ensuring comprehensive breakdown of cellular waste. The apoptotic signaling pathway involves the regulation of programmed cell death where Cathepsin D can influence the activation of downstream proteins like Bcl-2 and Bax which control cell survival.

The overexpression of Cathepsin D links to breast cancer and Alzheimer's disease. In breast cancer increased Cathepsin D expression correlates with tumor progression and metastasis influencing tumor behavior through interactions with other proteins involved in cell proliferation. Alzheimer's disease features the involvement of Cathepsin D in the breakdown of amyloid precursor protein which relates to amyloid beta plaque accumulation. The abnormal activity of Cathepsin D in these disorders makes it a potential target for therapeutic antibodies such as CTSD antibodies which aim to regulate its function.
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Product protocols

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Target data

Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation (PubMed : 27333034). Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
See full target information CTSD
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 17: PubMed39940321

2025

Enhancement of Lower Limb Muscle Strength and Reduction of Inflammation in the Elderly: A Randomized, Double-Blind Clinical Trial Comparing PS23 Probiotic with Heat-Treated Supplementation.

Applications

Unspecified application

Species

Unspecified reactive species

Mon-Chien Lee,Yi-Ju Hsu,Hung-Jen Yang,Chi-Chang Huang

American heart journal plus : cardiology research and practice 49:100489 PubMed39811721

2025

Cathepsin-D and outcomes in peripartum cardiomyopathy: Results from IPAC.

Applications

Unspecified application

Species

Unspecified reactive species

Vincenzo B Polsinelli,Karen Hanley-Yanez,Charles F McTiernan,Kalgi Modi,Jennifer Haythe,Hal Skopicki,Uri Elkayam,Leslie T Cooper,James D Fett,Dennis M McNamara

Nature chemical biology 21:577-587 PubMed39482469

2024

Carbonic anhydrase inhibition ameliorates tau toxicity via enhanced tau secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Lopez,Farah H Siddiqi,Julien Villeneuve,Rodrigo Portes Ureshino,Hee-Yeon Jeon,Philippos Koulousakis,Sophie Keeling,William A McEwan,Angeleen Fleming,David C Rubinsztein

Diabetes care 45:1416-1427 PubMed35377940

2022

Urinary Proteomics Identifies Cathepsin D as a Biomarker of Rapid eGFR Decline in Type 1 Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Christine P Limonte,Erkka Valo,Viktor Drel,Loki Natarajan,Manjula Darshi,Carol Forsblom,Clark M Henderson,Andrew N Hoofnagle,Wenjun Ju,Matthias Kretzler,Daniel Montemayor,Viji Nair,Robert G Nelson,John F O'Toole,Robert D Toto,Sylvia E Rosas,John Ruzinski,Niina Sandholm,Insa M Schmidt,Tomas Vaisar,Sushrut S Waikar,Jing Zhang,Peter Rossing,Tarunveer S Ahluwalia,Per-Henrik Groop,Subramaniam Pennathur,Janet K Snell-Bergeon,Tina Costacou,Trevor J Orchard,Kumar Sharma,Ian H de Boer

International journal of cosmetic science 43:275-282 PubMed33544395

2021

Relationship between lip skin biophysical and biochemical characteristics with corneocyte unevenness ratio as a new parameter to assess the severity of lip scaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jongwook Kim,Hyerin Yeo,Taeyoon Kim,Eui-Taek Jeong,Jun M Lim,Sun-Gyoo Park

European journal of heart failure 22:2102-2111 PubMed31797504

2019

The role of cathepsin D in the pathophysiology of heart failure and its potentially beneficial properties: a translational approach.

Applications

Unspecified application

Species

Unspecified reactive species

Martijn F Hoes,Jasper Tromp,Wouter Ouwerkerk,Nils Bomer,Silke U Oberdorf-Maass,Nilesh J Samani,Leong L Ng,Chim C Lang,Pim van der Harst,Hans Hillege,Stefan D Anker,Marco Metra,Dirk J van Veldhuisen,Adriaan A Voors,Peter van der Meer

Molecular genetics and metabolism 128:62-67 PubMed31358474

2019

Aberrant progranulin, YKL-40, cathepsin D and cathepsin S in Gaucher disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yuliya Afinogenova,Jiapeng Ruan,Ruhua Yang,Nathaniel Kleytman,Gregory Pastores,Andrew Lischuk,Pramod K Mistry

Osteoarthritis and cartilage 26:1078-1086 PubMed29852277

2018

Inflammatory and oxidative stress biomarkers in alkaptonuria: data from the DevelopAKUre project.

Applications

ELISA

Species

Unspecified reactive species

D Braconi,D Giustarini,B Marzocchi,L Peruzzi,M Margollicci,R Rossi,G Bernardini,L Millucci,J A Gallagher,K-H Le Quan Sang,R Imrich,J Rovensky,M Al-Sbou,L R Ranganath,A Santucci

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 33:402-408 PubMed28482048

2017

Neither creatinine- nor cystatin C-estimated glomerular filtration rate is optimal in oncology patients treated with targeted agents.

Applications

Unspecified application

Species

Unspecified reactive species

Tijl Vermassen,Karen Geboes,Marc De Man,Stéphanie Laurent,Elsie Decoene,Nicolaas Lumen,Joris Delanghe,Sylvie Rottey

The American journal of pathology 184:721-31 PubMed24434012

2014

The senescence-associated secretory phenotype promotes benign prostatic hyperplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Paz Vital,Patricia Castro,Susan Tsang,Michael Ittmann
View all publications
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