Human Cathepsin D (mature, single chain) ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human Cathepsin D (mature, single chain) with a sensitivity of 12.58 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, Heparin Plasma, Cell culture supernatant, Urine, Saliva, Milk
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 54.688 - 3500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 12.58 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation (PubMed:27333034). Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
Alternative names
CPSD, CTSD, Cathepsin D
What's included?
Human Cathepsin D (mature, single chain) ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human Cathepsin D (mature, single chain) with a sensitivity of 12.58 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, Heparin Plasma, Cell culture supernatant, Urine, Saliva, Milk
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 54.688 - 3500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 12.58 pg/mL
- General recovery
- 87%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. = 8.3 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. = 6.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 87 | Range 80 - 91 % |
Sample type Citrate plasma | Average % = 85 | Range 81 - 87 % |
Sample type Heparin Plasma | Average % = 85 | Range 83 - 88 % |
Sample type Cell culture supernatant | Average % = 113 | Range 104 - 117 % |
Sample type Urine | Average % = 102 | Range 101 - 102 % |
Sample type Saliva | Average % = 92 | Range 81 - 108 % |
Sample type Milk | Average % = 96 | Range 95 - 96 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Cathepsin D (mature, single chain) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cathepsin D (mature, single chain) protein in Serum, Cit Plasma, Hep Plasma, Cell culture supernatant, Cell culture supernatant, Urine, Saliva, Milk. Quantitate Human Cathepsin D (mature, single chain) with 12.58 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
- Also available in a fully validated 384-well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
Sandwich ELISA - Human Cathepsin D (mature, single chain) ELISA Kit- Extracellular (ab323516)
Interpolated concentrations of human Cathepsin D in HepG2 supernatant in 96-well vs. 384-well plates.
Interpolated concentration of native Cathepsin D was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 25% HepG2 supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.
Sandwich ELISA - Human Cathepsin D (mature, single chain) ELISA Kit- Extracellular (ab323516)
Example of human Cathepsin D standard curve in Sample Diluent NS + 1X Enhancer in 96-well vs. 384-well plate.
Example of human Cathepsin D standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Cathepsin D (mature, single chain) ELISA Kit- Extracellular (ab323516)
Interpolated concentrations of Cathepsin D in serum from donors with type 2 diabetes (T2D), compared to pooled normal human serum.
Serum of 3 individual human donors diagnosed with type 2 diabetes (T2D) was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Cathepsin D concentration of T2D samples was determined to be 1,975.95 pg/mL with a range of 1,314.15 – 2,776.70 pg/mL.
Sandwich ELISA - Human Cathepsin D (mature, single chain) ELISA Kit- Extracellular (ab323516)
Interpolated concentrations of Cathepsin D in normal human serum donors.
Serum of 12 individual healthy human donors (6 female and 6 male) was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Cathepsin D concentration of detected samples was determined to be 213.08 pg/mL with a range of undetected – 386.16 pg/mL.
Sandwich ELISA - Human Cathepsin D (mature, single chain) ELISA Kit- Extracellular (ab323516)
Interpolated concentrations of human Cathepsin D in cell culture supernatant and saliva.
Interpolated concentration of native Cathepsin D was measured in duplicate at different sample concentrations. Undiluted samples are as follows: A431 supernatant 6.25%, HepG2 supernatant 25%, and male saliva 25%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.
Sandwich ELISA - Human Cathepsin D (mature, single chain) ELISA Kit- Extracellular (ab323516)
Interpolated concentrations of human Cathepsin D in serum and plasma.
Interpolated concentration of native Cathepsin D was measured in duplicate at different sample concentrations. Undiluted samples are 50% serum, plasma (citrate), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.
Sandwich ELISA - Human Cathepsin D (mature, single chain) ELISA Kit- Extracellular (ab323516)
Example of human Cathepsin D standard curve in Sample Diluent SSW.
Example of human Cathepsin D standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Cathepsin D (mature, single chain) ELISA Kit- Extracellular (ab323516)
Example of human Cathepsin D standard curve in Sample Diluent NS + 1X Enhancer.
Example of human Cathepsin D standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
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