Human Cathepsin D (mature, single chain) ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human Cathepsin D (mature, single chain) with a sensitivity of 8.61 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 54.688 - 3500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 8.61 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation (PubMed:27333034). Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
Alternative names
CPSD, CTSD, Cathepsin D
What's included?
Human Cathepsin D (mature, single chain) ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human Cathepsin D (mature, single chain) with a sensitivity of 8.61 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 54.688 - 3500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 8.61 pg/mL
- General recovery
- 112%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. = 3.3 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. = 2.7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell Lysate | Average % = 112 | Range 106 - 121 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Cathepsin D (mature, single chain) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cathepsin D (mature, single chain) protein in Cell Lysates. Quantitate Human Cathepsin D (mature, single chain) with 8.61 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
- Also available in a fully validated 384-well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Sandwich ELISA - Human Cathepsin D (mature, single chain) ELISA Kit- Intracellular (ab323517)
Interpolated concentrations of human Cathepsin D in HepG2 cell extract in 96-well vs. 384-well plates.
Interpolated concentration of native Cathepsin D was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 500 µg/mL HepG2 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.
Sandwich ELISA - Human Cathepsin D (mature, single chain) ELISA Kit- Intracellular (ab323517)
Example of human Cathepsin D standard curve in 1X Cell Extraction Buffer SSW in 96-well vs. 384-well plate.
Example of human Cathepsin D standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Cathepsin D (mature, single chain) ELISA Kit- Intracellular (ab323517)
Interpolated concentrations of human Cathepsin D in cell extract.
Interpolated concentration of native Cathepsin D was measured in duplicate at different sample concentrations. Undiluted samples are as follows: HL-60 cell extract 125 µg/mL and HepG2 cell extract 500 µg/mL. The interpolated dilution factor corrected are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.
Sandwich ELISA - Human Cathepsin D (mature, single chain) ELISA Kit- Intracellular (ab323517)
Example of human Cathepsin D standard curve in 1X Cell Extraction Buffer SSW.
Example of human Cathepsin D standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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