AB323517

Human Cathepsin D (mature, single chain) ELISA Kit- Intracellular

SimpleStep
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Human Cathepsin D (mature, single chain) ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human Cathepsin D (mature, single chain) with a sensitivity of 8.61 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CPSD, CTSD, Cathepsin D

4 Images

Sandwich ELISA - Human Cathepsin D (mature, single chain) ELISA Kit- Intracellular (AB323517)

sELISA

Supplier Data

Sandwich ELISA - Human Cathepsin D (mature, single chain) ELISA Kit- Intracellular (AB323517)

Interpolated concentrations of human Cathepsin D in HepG2 cell extract in 96-well vs. 384-well plates.

Interpolated concentration of native Cathepsin D was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 500 µg/mL HepG2 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

sELISA

Supplier Data

Sandwich ELISA - Human Cathepsin D (mature, single chain) ELISA Kit- Intracellular (AB323517)

Interpolated concentrations of human Cathepsin D in cell extract.

Interpolated concentration of native Cathepsin D was measured in duplicate at different sample concentrations. Undiluted samples are as follows : HL-60 cell extract 125 µg/mL and HepG2 cell extract 500 µg/mL. The interpolated dilution factor corrected are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

sELISA

Supplier Data

Sandwich ELISA - Human Cathepsin D (mature, single chain) ELISA Kit- Intracellular (AB323517)

Example of human Cathepsin D standard curve in 1X Cell Extraction Buffer SSW in 96-well vs. 384-well plate.

Example of human Cathepsin D standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Cathepsin D (mature, single chain) ELISA Kit- Intracellular (AB323517)

Example of human Cathepsin D standard curve in 1X Cell Extraction Buffer SSW.

Example of human Cathepsin D standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 8.61 pg/mL

Range

54.688 - 3500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Cathepsin D (mature, single chain) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cathepsin D (mature, single chain) protein in Cell Lysates. Quantitate Human Cathepsin D (mature, single chain) with 8.61 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

Single-wash protocol reduces assay time to 90 minutes or less
High sensitivity, specificity and reproducibility from superior antibodies
Fully validated in biological samples
96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.3" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.7" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "106 - 121 %", "average": "= 112" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation (PubMed : 27333034). Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.

See full target information CTSD

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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