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AB313523

Human Cathepsin H ELISA Kit

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Human Cathepsin H ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Cathepsin H in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

CPSB, CTSH, Pro-cathepsin H

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Sandwich ELISA - Human Cathepsin H ELISA Kit (AB313523)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cathepsin H ELISA Kit (AB313523)

Example of standard curve using ab313523. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.17 ng/mL

Range

0.17 - 40 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Cathepsin H ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Cathepsin H in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Cathepsin H coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human C1qTNF9, Carbonic Anhydrase VA, CANT1, Contactin-5, Chymotrypsin C/CTRC, Draxin, EphB2, EphB3, FABP8, Fgr, FKBP51, FUCA1, Galanin, GALNT10, Gastrokine 1, Glyoxalase II, HS3ST1, HS3ST3B1, Lin28, Lysyl Oxidase Homolog, LRRTM4, Methionine Aminopeptidase 1D, Matrilin-2, MCEMP1, Mcl-1, MDGA2, MEF2C, Methionine Aminopeptidase 2, Neurocan, Nogo-A, PCK1, PlGF-2, PON1, SALM4/LRFN3, Semaphorin 6C, SorCS2, ST3GAL1, ST8SIA1, Tsukushi/TSK.

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Recovery

[ { "sample": "Serum", "range": "105 - 123 %", "average": "= 113" }, { "sample": "Plasma", "range": "69 - 107 %", "average": "= 85.07" }, { "sample": "Cell culture media", "range": "80 - 142 %", "average": "= 99.46" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313523-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313523-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Cathepsin H Protein Standard", "number":"AB313523-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313523-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Cathepsin H Antibody-coated ELISA Plate", "number":"AB313523-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313523-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"250X HRP-Streptavidin", "number":"AB313523-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Cathepsin H Detection Antibody", "number":"AB313523-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313523-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin H also known as CPSH is a lysosomal cysteine protease that plays a role in intracellular protein degradation. It has a molecular mass of approximately 28 kilodaltons. This enzyme is expressed across various tissues with high expression levels in liver kidney and spleen. The enzyme primarily functions at acidic pH levels within lysosomes where it acts to break down peptides into amino acids by removing terminal residues.
Biological function summary

As a member of the cathepsin family Cathepsin H contributes to the regulation of protein turnover and homeostasis. It performs a dual role as both an aminopeptidase and endopeptidase. In addition to its function in protein degradation the enzyme involves itself in antigen processing during immune responses. Reactivity of Cathepsin H in immune complexes shows its importance in shaping immune tolerance and responses.

Pathways

Cathepsin H interacts with the lysosomal degradation pathway and plays a role in the MHC class II-mediated antigen presentation. This pathway is pivotal for the regulation of immune responses. Relation to other proteins like Cathepsin L and Cathepsin B highlights Cathepsin H's complementary function in proteolytic processing within lysosomes. Its role and expression play an integral part in balancing protein synthesis and degradation cycles.

Cathepsin H associates closely with cancer and neurodegenerative diseases. Its activity can influence tumor progression and metastasis due to its role in extracellular matrix remodeling and cell migration. In neurodegenerative disorders such as Alzheimer's disease its dysregulated activity may contribute to neuronal cell death. Cathepsin H’s connection to proteins like amyloid precursor protein (APP) in Alzheimer's highlights the complex interplay between protease activity and disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Important for the overall degradation of proteins in lysosomes.
See full target information CTSH
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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