JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB287165

Human Cathepsin Z ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human Cathepsin Z ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Cathepsin Z with a sensitivity of 2.48 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Cathepsin Z, Cathepsin P, Cathepsin X, CTSZ

2 Images
Sandwich ELISA - Human Cathepsin Z ELISA Kit (AB287165)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cathepsin Z ELISA Kit (AB287165)

Interpolated concentrations of human Cathepsin Z in human serum, plasma (citrate), plasma (heparin) and plasma (EDTA).

The endogenous concentration of Cathepsin Z was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 200 human serum, plasma (citrate), plasma (heparin) and plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Cathepsin Z ELISA Kit (AB287165)
  • sELISA

Supplier Data

Sandwich ELISA - Human Cathepsin Z ELISA Kit (AB287165)

Example of human Cathepsin Z standard curve in Sample Diluent NS.

Example human Cathepsin Z standard curve. Background-subtracted data values (mean =/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.48 pg/mL

Range

10.94 - 700 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Assay Kits

AB119586

Human Cathepsin D ELISA Kit

Sandwich ELISA - Human Cathepsin D ELISA Kit (AB119586)

  • 0
  • 0
Assay Kits

AB155427

Human Cathepsin S ELISA Kit (CTSS)

Sandwich ELISA - Human Cathepsin S ELISA Kit (CTSS) (AB155427)

  • 0
  • 0
Assay Kits

AB272205

Human Cathepsin B ELISA Kit

Recombinant|SimpleStep

ELISA - Human Cathepsin B ELISA Kit (AB272205)

  • 0
  • 0
Primary Antibodies

AB180580

Anti-Cathepsin Z antibody [EPR14357]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin Z antibody [EPR14357] (AB180580)

  • 5
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human Cathepsin Z ELISA kit (ab287165) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Cathepsin Z protein in human serum and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cathepsin Z with 2.48 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "97 - 113 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "102 - 112 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "97 - 113 %", "average": "= 104" }, { "sample": "Citrate plasma", "range": "99 - 126 %", "average": "= 113" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab287165-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB287165-CMP11", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB287165-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB287165-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Cathepsin Z Lyophilized Recombinant Protein", "number":"AB287165-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Cathepsin Z Capture Antibody", "number":"AB287165-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB287165-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Cathepsin Z Detector Antibody", "number":"AB287165-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB287165-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB287165-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB287165-CMP07", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin Z also known as cathepsin X or CTSZ is a lysosomal cysteine protease with a molecular weight of approximately 34 kDa. It is expressed mainly in immune cells such as macrophages and dendritic cells but also found in other tissues including liver kidney and spleen. This protease performs proteolytic functions which involve cleaving peptide bonds in proteins contributing to protein catabolism in cells. The widespread expression of Cathepsin Z suggests its involvement in various physiological processes.
Biological function summary

Cathepsin Z participates in immune response regulation and cellular adhesion processes. It belongs to the papain-like cysteine protease family. Notably it associates with integrins to influence cell-extracellular matrix interactions affecting cell migration and adhesion. By modulating these processes Cathepsin Z impacts immune surveillance and tissue remodeling demonstrating its multifaceted role in maintaining cellular homeostasis.

Pathways

Cathepsin Z engages significantly in apoptotic and antigen-presenting pathways. In apoptosis it influences cell death by degrading specific substrates involved in cell survival. It also assists in the processing of antigens within dendritic cells facilitating effective antigen presentation to T cells. Related proteins such as Cathepsin S and Cathepsin L which also partake in lysosomal pathways further illustrate the shared functionality and redundancy within this protease family enabling a robust regulatory network.

Cathepsin Z has implications in cancer progression and neurodegenerative diseases. It is often overexpressed in cancerous tissues where it aids tumor cell invasion and metastasis through degradation of extracellular components. Additionally alterations in Cathepsin Z activity are linked to Alzheimer's disease where it may affect the processing of amyloid precursor proteins similar to its interaction with other proteins like Cathepsin B. These connections highlight the potential for Cathepsin Z as a target for therapeutic intervention in various pathological conditions.
style
left-column

Product protocols

style
left-column

Target data

Exhibits carboxy-monopeptidase as well as carboxy-dipeptidase activity (PubMed : 10504234). Capable of producing kinin potentiating peptides (By similarity).
See full target information Cathepsin Z
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com