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AB270881

Human Caveolin-2 ELISA Kit (CAV2)

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Human Caveolin-2 ELISA Kit (CAV2) is a single-wash 90-min Simplestep used to quantify Human Caveolin-2 (CAV2) with a sensitivity of 38.91 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Caveolin-2, CAV2

3 Images
Sandwich ELISA - Human Caveolin-2 ELISA Kit (CAV2) (AB270881)
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Supplier Data

Sandwich ELISA - Human Caveolin-2 ELISA Kit (CAV2) (AB270881)

Interpolated concentrations of native Caveolin-2 in human A549 and heart tissue homogenate extract based on a 500 μg/mL and 250 μg/mL extract loads, respectively.

The concentrations of Caveolin-2 were measured in duplicate and interpolated from the Caveolin-2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Caveolin-2 concentration was determined to be 1,974.86 pg/mL in A549 extract and 3,873.07 pg/mL in heart homogenate extract.

Sandwich ELISA - Human Caveolin-2 ELISA Kit (CAV2) (AB270881)
  • sELISA

Supplier Data

Sandwich ELISA - Human Caveolin-2 ELISA Kit (CAV2) (AB270881)

Example of human Caveolin-2 standard curve in 1X Cell Extraction Buffer PTR.

The Caveolin-2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Caveolin-2 ELISA Kit (CAV2) (AB270881)
  • sELISA

Supplier Data

Sandwich ELISA - Human Caveolin-2 ELISA Kit (CAV2) (AB270881)

Representative image of Human Caveolin-2 ELISA Kit (CAV2) (ab270881)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human Caveolin-2 ELISA Kit (CAV2) Capture Antibody

- 10X Human Caveolin-2 (CAV2) Detector Antibody

- 10X Human Caveolin-2 ELISA Kit (CAV2) Lyophilised Purified Protein (2 vials)

- Human Caveolin-2 ELISA Kit (CAV2) 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 38.91 pg/mL

Range

78.13 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB133484

Anti-Caveolin-2 antibody [EPR5471]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody [EPR5471] (AB133484)

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Proteins & Peptides

AB114515

Recombinant Human Caveolin-2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Caveolin-2 protein (GST tag N-Terminus) (AB114515)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Caveolin-2 ELISA kit (ab270881) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Caveolin-2 protein in human cell and tissue samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Caveolin-2 with 38.91 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.

Caveolin-2 is a scaffolding protein found in the inner surface of caveolae. CAV-2 and CAV-1 form a hetero-oligomeric complex involved in signal transduction, apoptosis, cell growth and lipid metabolism. CAV-2 and CAV-1 expression in breast cancer is associated with basal-like and triple-negative immunophenotype.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": null, "average": "= 115" }, { "sample": "Cell Lysate", "range": null, "average": "= 102" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab270881-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB270881-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB270881-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB270881-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB270881-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB270881-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB270881-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB270881-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB270881-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Caveolin-2 (CAV2) Lyophilized Recombinant Protein", "number":"AB270881-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Caveolin-2 (CAV2) Capture Antibody", "number":"AB270881-CMP01", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB270881-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Caveolin-2 (CAV2) Detector Antibody", "number":"AB270881-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Caveolin-2 often referred to as CAV2 is a 20-22 kDa integral membrane protein and an important component of caveolae small invaginations in the plasma membrane. It is mainly expressed in adipocytes endothelial cells pneumocytes and some smooth muscle cells. CAV2 works together with other caveolins primarily caveolin-1 to form these flask-shaped invaginations which play roles in various cellular processes.
Biological function summary

Caveolin-2 participates in modulating cellular functions such as signal transduction cholesterol homeostasis and endocytosis. As part of the caveolae complex it cooperates with caveolin-1 to regulate lipid raft domains influencing the movement and function of molecules within the cell. The presence of caveolin-2 helps maintain the caveolae structure and is involved in cellular responses to mechanical stress.

Pathways

Caveolin-2 is implicated in the regulation of insulin signaling and the Ras-MAPK signaling pathway. In the insulin signaling pathway it forms complexes with caveolin-1 and other proteins like IRS-1 affecting glucose uptake in cells. In the Ras-MAPK pathway caveolin-2 interacts with signaling molecules associated with the pathway modulating cell proliferation and survival.

Caveolin-2 has been linked to cancer development and pulmonary disorders. Its interactions with caveolin-1 and other signaling proteins in cancer cells can influence tumor growth and metastasis. Additionally altered expression of caveolin-2 affects pulmonary disorders such as emphysema by impacting endothelial cell function. Understanding these connections helps in exploring potential therapeutic interventions.
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Product protocols

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Target data

May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression (By similarity).
See full target information CAV2
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websiteProtocolBooklet
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Product promise

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