Human CCL14 ELISA Kit (HCC-1) is a single-wash 90-min Simplestep used to quantify Human CCL14 (HCC-1) with a sensitivity of 4.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Journal of intensive care 11:112023Analysis of urinary C-C motif chemokine ligand 14 (CCL14) and first-generation urinary biomarkers for predicting renal recovery from acute kidney injury: a prospective exploratory study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBen-Shu Qian,Hui-Miao Jia,Yi-Bing Weng,Xin-Cheng Li,Chao-Dong Chen,Fang-Xing Guo,Yu-Zhen Han,Li-Feng Huang,Yue Zheng,Wen-Xiong LiPubMed 36941674
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 5.5 - 350 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 4.4 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induces intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and is inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes, eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5.
Alternative names
NCC2, SCYA14, CCL14, C-C motif chemokine 14, Chemokine CC-1/CC-3, HCC-1(1-74), NCC-2, Small-inducible cytokine A14, HCC-1/HCC-3
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Human CCL14 ELISA Kit (HCC-1) is a single-wash 90-min Simplestep used to quantify Human CCL14 (HCC-1) with a sensitivity of 4.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 5.5 - 350 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 4.4 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 3.6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 4 | mean - | SD - | C.V. 5.1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 111 | Range 108 - 118 % |
Sample type EDTA Plasma | Average % = 112 | Range 109 - 117 % |
Sample type Saliva | Average % = 88 | Range 83 - 94 % |
Sample type Urine | Average % = 101 | Range 99 - 102 % |
Sample type Cell culture media | Average % = 98 | Range 95 - 100 % |
Sample type Heparin Plasma | Average % = 102 | Range 101 - 104 % |
Sample type Citrate plasma | Average % = 112 | Range 108 - 119 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human CCL14 ELISA kit (ab272201) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL14 protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CCL14 with 4.4 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
C-C motif chemokine 14 (CCL14), also known as HCC-1 and NCC-2, is a cytokine encoded by the gene CCL14. Mature secreted CCL14 is a 74- amino acid containing active glycoprotein and is constitutively expressed by multiple tissues and has been found at high concentrations in plasma.
CCl14 enhances the proliferation of CD34 myeloid progenitor cells and has weak activities on human monocytes through receptors that also recognize MIP-1 alpha. CCL14 induces intracellular Ca2+ changes and enzyme release but does not induce chemotaxis in monocytes.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CCL14 also known as HCC-1 or HCC-3 is a small cytokine belonging to the CC chemokine family. It has a molecular weight of approximately 8 kDa. The protein is found in a variety of tissues where it is secreted by immune cells fibroblasts and endothelial cells. CCL14 interacts with chemokine receptors to mediate cell signaling and can be detected at the interface of inflammatory responses. Its expression can be modulated during different immune responses.
- Biological function summary
The CCL14 protein acts as a chemoattractant for specific types of white blood cells including monocytes and lymphocytes which are critical in mounting immune responses. It does not function as a part of a larger complex but operates independently to influence immune cell trafficking. CCL14 is involved in modulating inflammatory reactions by recruiting immune cells to sites of tissue damage or infection. It helps orchestrate a coordinated immune response by activating target cells through its receptor binding.
- Pathways
CCL14 plays important roles within the immune response and inflammation pathways. It contributes to the chemokine signaling pathway facilitating the migration and activation of leukocytes. Another significant pathway involving CCL14 is the MAPK signaling pathway which it influences through interactions with other signaling molecules such as CCL3 and CCL5. These relationships highlight CCL14's roles in both promoting and controlling immune responses.
- Associated diseases and disorders
CCL14 is linked to various inflammatory and immune-related diseases. It shows a strong association with chronic inflammation disorders such as rheumatoid arthritis where it contributes to tissue infiltration by immune cells. CCL14 also plays a role in atherosclerosis where it can influence the recruitment of macrophages that contribute to plaque formation. Through these conditions CCL14 interacts with other proteins involved in these pathologies including CCL2 and CXCL8 which further modulate inflammatory processes.
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4 product images
Sandwich ELISA - Human CCL14 ELISA Kit (HCC-1) (ab272201)
Example of human CCL14 standard curve in Sample Diluent NS.
The CCL14 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human CCL14 ELISA Kit (HCC-1) (ab272201)
Interpolated concentrations of native CCL14 in human serum, plasma (EDTA), plasma (citrate), plasma (heparin).
The concentrations of CCL14 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:200, plasma (EDTA) 1:200, plasma (citrate) 1:200, plasma (heparin) 1:400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 59.0 ng/mL in serum, 43.1 ng/mL in plasma (EDTA), 48.8 ng/mL in and plasma (citrate), and 98.9 ng/mL in plasma (heparin).
Sandwich ELISA - Human CCL14 ELISA Kit (HCC-1) (ab272201)
Interpolated concentrations of native CCL14 in human saliva and urine.
The concentrations of CCL14 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: serum, plasma (EDTA), plasma – (citrate), plasma – (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 96.7 pg/mL in saliva and 87.9 pg/ml in urine.
Sandwich ELISA - Human CCL14 ELISA Kit (HCC-1) (ab272201)
Serum from eight individual healthy CCL14 donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL14 concentration was determined to be 33.3 ng/mL with a range of 20.5 – 56.5 ng/mL.
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