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AB243673

Human CCL16 ELISA Kit

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Human CCL16 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CCL16 with a sensitivity of 1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

ILINCK, NCC4, SCYA16, CCL16, C-C motif chemokine 16, Chemokine CC-4, Chemokine LEC, IL-10-inducible chemokine, LCC-1, Liver-expressed chemokine, Lymphocyte and monocyte chemoattractant, Monotactin-1, NCC-4, Small-inducible cytokine A16, HCC-4, LMC, MTN-1

2 Images
Sandwich ELISA - Human CCL16 ELISA Kit (AB243673)
  • sELISA

Supplier Data

Sandwich ELISA - Human CCL16 ELISA Kit (AB243673)

Example of human CCL16 standard curve in Sample Diluent NS.

The CCL16 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CCL16 ELISA Kit (AB243673)
  • sELISA

Supplier Data

Sandwich ELISA - Human CCL16 ELISA Kit (AB243673)

Interpolated concentrations of native CCL16 in human serum, plasma and cell culture supernatant samples.

The concentrations of CCL16 were measured in duplicates, interpolated from the CCL16 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1%, plasma (citrate) 1.5%, and HepG2 supernatant 30%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL16 concentration was determined to be 15284.9 pg/mL in serum, 12472.9 pg/mL in plasma (citrate) and 599.3 pg/mL in HepG2 supernatant.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1 pg/mL

Range

2.34 - 150 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CCL16 ELISA Kit (ab243673) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL16 protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CCL16 with 1 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.7" }, { "reproducibilityType": "Intra", "sample": "Sample", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.9" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "103 - 121 %", "average": "= 110" }, { "sample": "Serum", "range": "81 - 128 %", "average": "= 110" }, { "sample": "Citrate plasma", "range": "80 - 117 %", "average": "= 97" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LEC also known as CCL16 is a chemokine protein that functions in the immune system. This protein weighs approximately 22 kDa and resides mainly in the liver and peripheral blood leukocytes. LEC belongs to the CC chemokine family characterized by two adjacent cysteine residues. The protein connects with other cellular systems to modulate immune responses. Antibodies like anti-LEC can detect it effectively and commercial products such as LEC BSA and LEC custom products aid in its study.
Biological function summary

LEC performs vital roles in immune cell signaling and chemotaxis. It binds specifically to certain chemokine receptors on target cells initiating signaling cascades that recruit lymphocytes to infected or inflamed tissues. LEC functions as part of a larger complex that includes other chemokines to coordinate immune responses. Its expression and activity influence various immune functions which makes its regulation significant for maintaining healthy immune system operation.

Pathways

LEC acts within immune signaling pathways and inflammation-related processes. It plays essential parts in pathways like the chemokine signaling pathway where it interacts with other proteins such as CCR1 and CCR2 chemokine receptors. Through these interactions LEC contributes to the recruitment and activation of specific immune cell subtypes facilitating a coordinated response to inflammation or infection.

LEC connects to conditions involving immune dysregulation like rheumatoid arthritis and liver diseases. In rheumatoid arthritis LEC levels can affect inflammation and tissue damage often in concert with proteins like TNF-alpha. In liver diseases altered LEC expression may relate to liver fibrosis with connections to proteins such as TGF-beta. Understanding LEC's role in these conditions can lead to better therapeutic strategies targeting the chemokine network.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Shows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelosuppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant SCYA16 shows chemotactic activity for monocytes and THP-1 monocytes, but not for resting lymphocytes and neutrophils. Induces a calcium flux in THP-1 cells that were desensitized by prior expression to RANTES.
See full target information CCL16
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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websiteProtocolBooklet
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Associated Products

Select an associated product type
Alternative Version
Assay Kits

AB256630

Human CCL16 Antibody Pair - BSA and Azide free

matched-antibody-pair-kits

human-ccl16-antibody-pair-bsa-and-azide-free-ab256630

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