Human CCL18 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CCL18 with a sensitivity of 0.55 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1.56 - 100 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.55 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses.
Alternative names
AMAC1, DCCK1, MIP4, PARC, SCYA18, CCL18, C-C motif chemokine 18, Alternative macrophage activation-associated CC chemokine 1, CC chemokine PARC, Dendritic cell chemokine 1, Macrophage inflammatory protein 4, Pulmonary and activation-regulated chemokine, Small-inducible cytokine A18, AMAC-1, DC-CK1, MIP-4
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Human CCL18 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CCL18 with a sensitivity of 0.55 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1.56 - 100 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.55 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 3 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 7.7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 92 | Range 87 - 95 % |
Sample type Serum | Average % = 90 | Range 85 - 93 % |
Sample type Urine | Average % = 90 | Range 86 - 94 % |
Sample type Citrate plasma | Average % = 103 | Range 97 - 111 % |
Sample type EDTA Plasma | Average % = 93 | Range 87 - 96 % |
Sample type Heparin Plasma | Average % = 99 | Range 92 - 102 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human CCL18 ELISA Kit (ab211649) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CCL18 protein in cell culture supernatant, plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CCL18 with 0.55 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Human CCL18 (macrophage inflammatory protein-4) belongs to the C-C motif containing chemokine family and contains a 20 amino acid (aa) signal peptide and a 69 aa residue mature protein. CCL18 was identified as a chemotactic factor for naïve T-cells, CD4+ and CD8+ T-cells, and nonactivated lymphocytes, suggesting that it plays an important role in immune responses. Additionally, MIP4 is mainly induced by Th2 type cytokines, such as IL-4 and IL-13, and inhibited by IFN-γ. High expression of MIP4 is seen in lung, lymph nodes, placenta, and macrophages derived from peripheral blood monocytes. Additionally, MIP4 is found in high levels in serum, plasma, and synovial fluid and in low levels in urine. Currently, a rodent homolog has not been identified.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CCL18 also known as pulmonary and activation-regulated chemokine (PARC) is a chemokine protein with a molecular weight of about 8.8 kDa. It is mainly expressed in the lung but also found in lymph nodes and placenta. CCL18 is secreted by activated macrophages and dendritic cells. As a chemokine it plays a role in immune system signaling where it attracts immune cells like T lymphocytes. Researchers utilize CCL18 ELISA kits to accurately measure its levels in biological samples providing insight into its functions.
- Biological function summary
CCL18 plays an important role in modulating immune responses and recruiting immune cells to sites of inflammation. It is not part of a larger complex but works closely with other components of the immune system. CCL18 influences the activity of monocytes and the differentiation of T cells which are essential for mounting effective defense against pathogens. In inflammatory conditions increased CCL18 levels suggest its importance in controlling the magnitude and duration of immune responses.
- Pathways
CCL18 is involved in the immune response and inflammatory pathways. It works particularly in chemokine signaling pathways and shares functional relations with proteins like CCR3 and CCR6 which act as receptors for various chemokines. The binding of CCL18 to these receptors on targeted cells facilitates the chemotaxis of immune cells steering the immune response to appropriate areas in the body where they are needed.
- Associated diseases and disorders
CCL18 has known associations with pulmonary fibrosis and various cancers. In pulmonary fibrosis CCL18 levels correlate with disease severity and progression indicating its potential role as a biomarker. The protein interacts with other signaling molecules including collagen and matrix metalloproteinases driving the fibrotic process. In cancers especially breast cancer high CCL18 expression often corresponds to tumor progression and metastasis. These interactions highlight its relevance not only in disease progression but also as a target for therapeutic interventions.
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7 product images
Sandwich ELISA - Human CCL18 ELISA Kit (ab211649)
Human MIP4 standard curve comparison data.
Standard curve comparison between human MIP4 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 6-fold increase in sensitivity.
Sandwich ELISA - Human CCL18 ELISA Kit (ab211649)
Example of human MIP4 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human CCL18 ELISA Kit (ab211649)
Interpolated concentrations of native MIP4 in human serum and plasma samples.
The concentrations of MIP4 were measured in duplicates, interpolated from the MIP4 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:1,200, plasma (citrate) 1:600, plasma (EDTA) 1:600, and plasma (heparin) 1:1,200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP4 concentration was determined to be 79,615 pg/mL in serum, 57,039 pg/mL in plasma (citrate), 59,254 pg/mL in plasma (EDTA) and 71,426 pg/mL in plasma (heparin).
Sandwich ELISA - Human CCL18 ELISA Kit (ab211649)
Interpolated concentrations of native CCL18 in human urine, synovial fluid, and PBMC stimulated media samples.
The concentrations of CCL18 were measured in duplicates, interpolated from the CCL18 standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 50% and synovial fluid 1:600. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL18 concentration was determined to be 34.5 pg/mL in urine and 57.6 ng/mL in synovial fluid.
Sandwich ELISA - Human CCL18 ELISA Kit (ab211649)
Interpolated concentrations of native CCL18 in human PBMC stimulated media samples.
The concentrations of CCL18 were measured in duplicates, interpolated from the CCL18 standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC stimulated media (Donor 1) 6.25% and PBMC stimulated media (Donor 2) 3.13%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CCL18 concentration was determined to be 1.25 ng/mL in PBMC stimulated media (Donor 1) and 2.5 ng/mL in PBMC stimulated media (Donor 2). PBMC samples were cultured in RPMI media with 10% fetal bovine serum and 1% PenStrep and stimulated with 1.5% PHA-M for 48 hours.
Sandwich ELISA - Human CCL18 ELISA Kit (ab211649)
Interpolated concentrations of native MIP4 in unstimulated versus stimulated samples of PBMC media.
All PBMC media samples were diluted to 3.13% and analyzed. The concentrations of MIP4 were measured in duplicates and interpolated from the MIP4 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP4 concentration was determined to be 46 pg/mL in unstimulated PBMC Media (Donor 1), 1,292 pg/mL in stimulated PBMC Media (Donor 1), 87 pg/mL in PBMC unstimulated media (Donor 2), and 2,521 pg/mL in PBMC stimulated media (Donor 2). PBMC samples were cultured in RPMI media with 10% fetal bovine serum and 1% PenStrep (unstimulated samples). Then PBMC samples were stimulated with 1.5% PHA-M for 48 hours.
Sandwich ELISA - Human CCL18 ELISA Kit (ab211649)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP4 concentration was determined to be 47,858 pg/mL with a range of 29,081 – 66,007 pg/mL.
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