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AB100611

Human CCL23 ELISA Kit

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(2 Publications)

Human CCL23 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CCL23 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

MIP3, MPIF1, SCYA23, CCL23, C-C motif chemokine 23, CK-beta-8, Macrophage inflammatory protein 3, Myeloid progenitor inhibitory factor 1, Small-inducible cytokine A23, CKB-8, MIP-3, MPIF-1

2 Images
Sandwich ELISA - Human CCL23 ELISA Kit (AB100611)
  • sELISA

Supplier Data

Sandwich ELISA - Human CCL23 ELISA Kit (AB100611)

Representative Standard Curve using ab100611.

Sandwich ELISA - Human CCL23 ELISA Kit (AB100611)
  • sELISA

Supplier Data

Sandwich ELISA - Human CCL23 ELISA Kit (AB100611)

Representative Standard Curve using ab100611.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 7 pg/mL

Range

8.23 - 6000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's CCL23 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CCL23 in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human CCL23 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL23 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CCL23 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL23 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get higher sensitivity in only 90 minutes with Human CCL23 ELISA Kit (ab216169) from our SimpleStep ELISA® range.

Optimisation may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "81 - 105 %", "average": "= 93.48" }, { "sample": "Serum", "range": "82 - 105 %", "average": "= 92.78" }, { "sample": "Plasma", "range": "83 - 106 %", "average": "= 94.16" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100611-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100611-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100611-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100611-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human CCL23 Standard (lyophilized)", "number":"AB100611-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100611-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100611-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CCL23 Microplate (12 x 8 wells)", "number":"AB100611-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100611-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human CCL23", "number":"AB100611-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCL23 also known as macrophage inflammatory protein 3 is a chemokine involved in immune response regulation. It has a molecular weight of approximately 15-16 kDa and is secreted by various cells such as macrophages dendritic cells and endothelial cells. CCL23 is expressed mostly in the bone marrow and lymph nodes but it also localizes in tissues like the liver and lungs under certain conditions. It plays an important role in mediating chemotaxis directing the migration of cells toward infection sites.
Biological function summary

CCL23 functions in the recruitment of immune cells especially eosinophils and monocytes to areas where immune responses are necessary. This chemokine is not known to be part of a larger protein complex but its ability to interact with specific receptors on cell surfaces makes it integral to immune signaling. During immune activation CCL23 contributes to the inflammatory cascade influencing both acute and chronic inflammatory responses.

Pathways

The signaling functions of CCL23 integrate within the chemokine signaling pathway and the broader immune response pathway. CCL23 interacts closely with proteins such as CCR1 which acts as its receptor and influences cellular responses via this receptor-mediated signaling. Additionally CCL23 shares mechanistic parallels with other chemokines like CCL22 though they may attract different subsets of cells they participate in coordinated immune activities.

CCL23 has relevance to conditions such as rheumatoid arthritis and allergic reactions. In these diseases elevated levels of CCL23 and its interaction with other proteins such as CCR1 contribute to the recruitment and activation of inflammatory cells. This chemokine's role in immune cell trafficking and inflammation makes it a target for therapeutic interventions aimed at modulating immune responses in such disorders.
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Product protocols

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Target data

Shows chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than CCL23.
See full target information CCL23
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer science 112:3099-3110 PubMed34050704

2021

CCL23 suppresses liver cancer progression through the CCR1/AKT/ESR1 feedback loop.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Meng,Lianghai Wang,Jun Hou,Xiaofeng Yang,Ke Lin,Hongxing Nan,Man Li,Xiangwei Wu,Xueling Chen

Scientific reports 9:5121 PubMed30914768

2019

A multi-stage association study of plasma cytokines identifies osteopontin as a biomarker for acute coronary syndrome risk and severity.

Applications

Unspecified application

Species

Unspecified reactive species

Kuai Yu,Binyao Yang,Haijing Jiang,Jun Li,Kai Yan,Xuezhen Liu,Lue Zhou,Handong Yang,Xiulou Li,Xinwen Min,Ce Zhang,Xiaoting Luo,Wenhua Mei,Shunchang Sun,Liyun Zhang,Xiang Cheng,Meian He,Xiaomin Zhang,An Pan,Frank B Hu,Tangchun Wu
View all publications
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