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AB99988

Human CCL28 ELISA Kit

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(3 Publications)

Human CCL28 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CCL28 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

SCYA28, CCL28, C-C motif chemokine 28, Mucosae-associated epithelial chemokine, Protein CCK1, Small-inducible cytokine A28, MEC

2 Images
Sandwich ELISA - Human CCL28 ELISA Kit (AB99988)
  • sELISA

Supplier Data

Sandwich ELISA - Human CCL28 ELISA Kit (AB99988)

Representative Standard Curve using ab99988

Sandwich ELISA - Human CCL28 ELISA Kit (AB99988)
  • sELISA

Supplier Data

Sandwich ELISA - Human CCL28 ELISA Kit (AB99988)

Representative Standard Curve using ab99988

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 25 pg/mL

Range

27.4 - 20000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's CCL28 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CCL28 in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human CCL28 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL28 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman CCL28 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL28 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "59 - 81 %", "average": "= 67.7" }, { "sample": "Serum", "range": "60 - 98 %", "average": "= 70.6" }, { "sample": "Plasma", "range": "59 - 82 %", "average": "= 68.6" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab99988-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"CCL28 Microplate (12 strips x 8 wells)", "number":"AB99988-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB99988-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human CCL28", "number":"AB99988-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB99988-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB99988-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB99988-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB99988-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human CCL28 Standard (lyophilized)", "number":"AB99988-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB99988-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCL28 also known as MEC (Mucosae-associated epithelial chemokine) is a chemokine weighing around 12 kDa. It plays a mechanical role in mediating cell chemotaxis promoting the migration of cells towards it. This chemokine predominantly expresses in mucosal tissues including the salivary glands intestines and respiratory tract epithelia. CCL28 interacts specifically with chemokine receptors CCR10 and CCR3 guiding immune cells to these mucosal surfaces where they perform important immune functions.
Biological function summary

CCL28 plays an important role in mucosal immunity and is involved in the defense against pathogens in these environments. The chemokine attracts lymphocytes and neutrophils aiding in their positioning at mucosal sites. This activity assists in maintaining a balanced immune response preventing excessive inflammation while ensuring adequate pathogen clearance. CCL28 is not part of a larger protein complex but performs critical functions independently coordinating with other immune mediators for effective response.

Pathways

The function of CCL28 aligns with its involvement in immune signaling and mucosal defense pathways. The chemokine integrates into the immune response network working closely with other chemokines and cytokines like CCL27 and IL-8. It engages in the chemokine signaling pathway which regulates leukocyte migration and positioning. The interplay between CCL28 and these immune mediators ensures proper protection of mucosal surfaces from potential pathogens.

CCL28 holds significant relevance in inflammatory bowel disease (IBD) and allergic rhinitis. In IBD the chemokine contributes to the infiltration of immune cells into the inflamed gut mucosa where its interaction with other cytokines such as TNF-α exacerbates tissue inflammation. In allergic rhinitis CCL28 participates in recruiting eosinophils to the nasal mucosa often correlating with heightened inflammatory responses in these patients and cooperating with proteins like IL-5 to sustain the allergic reaction.
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Product protocols

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Target data

Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner.
See full target information CCL28
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular cancer 22:206 PubMed38093346

2023

Dynamic altruistic cooperation within breast tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammad Sufyan Bin Masroni,Kee Wah Lee,Victor Kwan Min Lee,Siok Bian Ng,Chao Teng Law,Kok Siong Poon,Bernett Teck-Kwong Lee,Zhehao Liu,Yuen Peng Tan,Wee Ling Chng,Steven Tucker,Lynette Su-Mien Ngo,George Wai Cheong Yip,Min En Nga,Susan Swee Shan Hue,Thomas Choudary Putti,Boon Huat Bay,Qingsong Lin,Lihan Zhou,Mikael Hartman,Tze Ping Loh,Manikandan Lakshmanan,Sook Yee Lee,Vinay Tergaonkar,Huiwen Chua,Adeline Voon Hui Lee,Eric Yew Meng Yeo,Mo-Huang Li,Chan Fong Chang,Zizheng Kee,Karen Mei-Ling Tan,Soo Yong Tan,Evelyn Siew-Chuan Koay,Marco Archetti,Sai Mun Leong

Scientific reports 6:27152 PubMed27250766

2016

Hypoxia induced CCL28 promotes angiogenesis in lung adenocarcinoma by targeting CCR3 on endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Guichun Huang,Leilei Tao,Sunan Shen,Longbang Chen

Transfusion 55:1008-12 PubMed25412854

2014

Plasma CC-chemokine ligand 28 level is correlated with hematopoietic stem cells in human cord blood.

Applications

Unspecified application

Species

Unspecified reactive species

Jong Hyun Yoon,Sohee Oh,Sue Shin,Eun Youn Roh,Hye Ryun Lee,Soo Hyun Seo,Hyunwoong Park,Eun Young Song
View all publications
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