AB217773

Human CD105 ELISA Kit

Recombinant
SimpleStep
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Human CD105 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD105 with a sensitivity of 11.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD105, END, ENG, Endoglin

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Sandwich ELISA - Human CD105 ELISA Kit (AB217773)

sELISA

Supplier Data

Sandwich ELISA - Human CD105 ELISA Kit (AB217773)

Serum levels of CD105 are increased during the third trimester of pregnancy, especially in women with pre-eclampsia.

Serum from 10 healthy (not pregnant) female individuals, 5 healthy females in the third trimester of pregnancy (pooled), and 1 individual female in the third trimester showing signs of mild pre-eclampsia were tested for levels of CD105. The concentrations of CD105 were measured from serum in samples in 3 different dilutions in duplicates and interpolated from the CD105 standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean CD105 concentration was determined to be 61,333 pg/mL in healthy female donor serum, 30,8945 pg/mL in healthy pregnant donor serum, and 703,119 pg/mL in pre-eclampsia patient serum.

sELISA

Supplier Data

Sandwich ELISA - Human CD105 ELISA Kit (AB217773)

Interpolated concentrations of native CD105 in human serum and plasma samples.

The concentrations of CD105 were measured in duplicates, interpolated from the CD105 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 2.225%, plasma (citrate) 2.225%, plasma (heparin) 2.225% and plasma (EDTA) 2.225%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD105 concentration was determined to be 94,646 pg/mL in neat serum, 71,971 pg/mL in neat plasma (citrate), 75,408 pg/mL in neat plasma (heparin), and 76,842 pg/mL in neat plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Human CD105 ELISA Kit (AB217773)

Interpolated concentrations of native CD105 in HUVEC cell extract, human placenta tissue extract and human heart tissue extract.

Interpolated concentrations of native CD105 in HUVEC cell extract based on a 40 μg/mL extract load, human placenta tissue extract based on a 90 μg/mL extract load, and human heart tissue extract based on a 60 μg/mL extract load. The concentrations of CD105 were measured in duplicate and interpolated from the CD105 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD105 concentration was determined to be 2548 pg/mL in HUVEC cell extract, 542.9 pg/mL in placenta tissue extract, and 528.1 pg/mL in heart tissue extract.

sELISA

Supplier Data

Sandwich ELISA - Human CD105 ELISA Kit (AB217773)

Serum from ten individual healthy female human donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD105 concentration was determined to be 61,333 pg/mL with a range of 30,103 – 91,256 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human CD105 ELISA Kit (AB217773)

Example of human CD105 standard curve in Sample Diluent NS + Enhancer.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human CD105 ELISA Kit (AB217773)

Human CD105 standard curve comparison data.

Standard curve comparison between human CD105 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human CD105 ELISA Kit (AB217773)

Example of human CD105 standard curve in 1X Cell Extraction Buffer PTR + Enhancer.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 11.1 pg/mL

Range

78.125 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human CD105 ELISA Kit (ab217773) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD105 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD105 with 11.1 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

CD105 is a major glycoprotein of vascular endothelium. CD105 is involved in the regulation of angiogenesis. It may play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. CD105 acts as TGF-beta co-receptor and is involved in the TGF-beta/BMP signaling cascade. It is required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and it modulates TGF-beta1 signaling through SMAD3. CD105 is a homodimer that forms a heteromeric complex with the signaling receptors for transforming growth factor-beta: TGFBR1 and/or TGFBR2. It is able to bind TGF-beta 1, and 3 efficiently and TGF-beta 2 less efficiently. Circulating soluble form of CD105 is elevated in patients with preeclampsia, atherosclerosis and certain cancers including breast, colorectal, and myeloid malignancies.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Human Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" }, { "reproducibilityType": "Intra", "sample": "Human Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.8" } ]

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Recovery

[ { "sample": "Serum", "range": "84 - 120 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "84 - 119 %", "average": "= 100" }, { "sample": "Cell culture extracts", "range": "99 - 110 %", "average": "= 103" }, { "sample": "Tissue Extracts", "range": "96 - 119 %", "average": "= 106" }, { "sample": "Heparin Plasma", "range": "71 - 113 %", "average": "= 88" }, { "sample": "Citrate plasma", "range": "74 - 84 %", "average": "= 80" }, { "sample": "Cell culture media", "range": "95 - 116 %", "average": "= 107" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD105 also known as endoglin or the CD105 marker is a transmembrane glycoprotein with a molecular weight of approximately 180 kDa. It is a component of the TGF-beta receptor complex and exists in endothelial cells where it is abundantly expressed. Expression of CD105 is higher in proliferating cells particularly in the vasculature. You can also find it in tissues involved in the formation and remodeling of blood vessels such as during angiogenesis.

Biological function summary

Endoglin functions in the regulation of angiogenesis and vascular remodeling. It plays a significant role in mediating cellular responses to TGF-beta signaling influencing endothelial cell proliferation and migration. While not part of a larger structural complex endoglin interacts with receptors and signaling molecules important for vascular development and repair processes. This involvement aids in maintaining endothelial integrity and function under various physiological conditions.

Pathways

CD105 participates in the TGF-beta signaling and angiogenesis pathways. In these pathways it acts in conjunction with other proteins like TGF-beta receptors which play roles in cell differentiation proliferation and apoptosis. The interaction between CD105 and TGF-beta signaling regulates numerous cellular mechanisms impacting angiogenesis and cellular responses to environmental changes.

CD105 has links to hereditary hemorrhagic telangiectasia (HHT) and certain cancers. In HHT mutations in the endoglin gene alter vascular structure leading to the formation of abnormal blood vessels. Oncologically overexpression of CD105 is present in tumor angiogenesis aiding in the progression of certain cancers. Other proteins like VEGF and TGF-beta closely interact with endoglin influencing disease progression and presenting potential targets for therapeutic intervention.

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Product protocols

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Target data

Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed : 21737454, PubMed : 23300529). Required for normal structure and integrity of adult vasculature (PubMed : 7894484). Regulates the migration of vascular endothelial cells (PubMed : 17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (By similarity). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (By similarity). May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors (PubMed : 1692830). Acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed : 21737454, PubMed : 22347366, PubMed : 23300529, PubMed : 8370410). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (PubMed : 21737454, PubMed : 22347366, PubMed : 23300529).

See full target information ENG

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human CD105 ELISA Kit

Sandwich ELISA - Human CD105 ELISA Kit (AB217773)

Sandwich ELISA - Human CD105 ELISA Kit (AB217773)

Sandwich ELISA - Human CD105 ELISA Kit (AB217773)

Sandwich ELISA - Human CD105 ELISA Kit (AB217773)

Sandwich ELISA - Human CD105 ELISA Kit (AB217773)

Sandwich ELISA - Human CD105 ELISA Kit (AB217773)

Sandwich ELISA - Human CD105 ELISA Kit (AB217773)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise