Human CD127 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD127 with a sensitivity of 15.23 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 46.88 - 3000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 15.23 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).
Alternative names
CD127, Interleukin-7 receptor subunit alpha, IL-7 receptor subunit alpha, IL-7R subunit alpha, IL-7R-alpha, IL-7RA, CDw127, IL7R
What's included?
Recommended products
Human CD127 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD127 with a sensitivity of 15.23 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 46.88 - 3000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 15.23 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 4.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 4.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 98 | Range 95 - 100 % |
Sample type EDTA Plasma | Average % = 93 | Range 91 - 97 % |
Sample type Cell culture media | Average % = 93 | Range 89 - 97 % |
Sample type Heparin Plasma | Average % = 92 | Range 87 - 97 % |
Sample type Citrate plasma | Average % = 98 | Range 93 - 105 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human CD127 ELISA kit (ab270880) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD127 protein in human serum, plasma and cell and tissue supernatant sampls. It uses our proprietary SimpleStep ELISA® technology. Quantitate human CD127 with 15.23 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
CD127 is a component of the IL-7R heterodimer. CD127 is found on a variety of cells, including naïve and memory T cells, and is a receptor for IL-7 and TSLP. Mutations in CD127 have been linked to an increased susceptibility to multiple sclerosis, rheumatic arthritis, and T-cell acute lymphoblastic leukemia.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD127 also known as IL-7R alpha or IL-7Rα is a protein found on the surface of cells involved in key immune functions. The CD127 protein has an approximate mass of 55-60 kDa. It is a component of the interleukin-7 receptor which also includes the common gamma chain. You can find CD127 expressed on various immune cells like T cells B cells and dendritic cells. As a marker CD127 is an important indicator of T cell maturity and function. Researchers often target CD127 in studies using antibodies in assay formats like ELISA to measure its expression level and function.
- Biological function summary
CD127 plays an important role in regulating lymphocyte development and survival. It is part of the IL-7R complex partnering with IL-7 for signal transduction that promotes cell proliferation and survival. This interaction is essential for the development and homeostasis of T cells and B cells. In the absence of proper CD127 signaling lymphocytes may suffer from poor survival impacting overall immune competence. The IL-7-CD127 interaction informs researchers about mechanisms of immune modulation and resilience.
- Pathways
CD127 participates in the JAK/STAT signaling pathway which is pivotal for transmitting information from extracellular signals to the cell nucleus influencing gene expression. This pathway plays a vital role in immune cell response. When IL-7 binds to the IL-7R complex on CD127 it initiates a cascade that involves proteins like JAK1 and STAT5. These proteins mediate responses necessary for cell proliferation and differentiation within immune system networks.
- Associated diseases and disorders
CD127 has implications in conditions like severe combined immunodeficiency (SCID) and autoimmune diseases. In SCID mutations or defects in CD127 cause impaired T cell development leading to compromised immune function. Additionally CD127 dysregulation can contribute to autoimmune disorders by affecting T regulatory cells creating an imbalance in immune response. Ocrevus a monoclonal antibody therapy used for multiple sclerosis indirectly relates to CD127 by targeting the B cells which rely on IL-7R signaling mechanisms for survival and function. Researchers continue to investigate CD127 to better understand its role in immune-related diseases.
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3 product images
Sandwich ELISA - Human CD127 ELISA Kit (ab270880)
Example of human CD127 standard curve in Sample Diluent NS.
The CD127 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. The CD127 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human CD127 ELISA Kit (ab270880)
Interpolated concentrations of native CD127 in human serum and plasma samples.
The concentrations of CD127 were measured in duplicates, interpolated from the CD127 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 20%, plasma (citrate) 50%, plasma (heparin) 25%, and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD127 concentration was determined to be 7.89 ng/mL in serum, 5.56 ng/mL in plasma (citrate), 7.56 ng/ml in plasma (heparin), and 12.55 ng/mL in plasma (EDTA).
Sandwich ELISA - Human CD127 ELISA Kit (ab270880)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD127 concentration was determined to be 7.09 ng/mL with a range of 3.69 – 11.63 ng/mL.
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