AB270880

Human CD127 ELISA Kit

Recombinant
SimpleStep
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Human CD127 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD127 with a sensitivity of 15.23 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD127, Interleukin-7 receptor subunit alpha, IL-7 receptor subunit alpha, IL-7R subunit alpha, IL-7R-alpha, IL-7RA, CDw127, IL7R

3 Images

Sandwich ELISA - Human CD127 ELISA Kit (AB270880)

sELISA

Supplier Data

Sandwich ELISA - Human CD127 ELISA Kit (AB270880)

Interpolated concentrations of native CD127 in human serum and plasma samples.

The concentrations of CD127 were measured in duplicates, interpolated from the CD127 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 20%, plasma (citrate) 50%, plasma (heparin) 25%, and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD127 concentration was determined to be 7.89 ng/mL in serum, 5.56 ng/mL in plasma (citrate), 7.56 ng/ml in plasma (heparin), and 12.55 ng/mL in plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Human CD127 ELISA Kit (AB270880)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD127 concentration was determined to be 7.09 ng/mL with a range of 3.69 – 11.63 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human CD127 ELISA Kit (AB270880)

Example of human CD127 standard curve in Sample Diluent NS.

The CD127 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. The CD127 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 15.23 pg/mL

Range

46.88 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human CD127 ELISA kit (ab270880) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD127 protein in human serum, plasma and cell and tissue supernatant sampls. It uses our proprietary SimpleStep ELISA® technology. Quantitate human CD127 with 15.23 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.

CD127 is a component of the IL-7R heterodimer. CD127 is found on a variety of cells, including naïve and memory T cells, and is a receptor for IL-7 and TSLP. Mutations in CD127 have been linked to an increased susceptibility to multiple sclerosis, rheumatic arthritis, and T-cell acute lymphoblastic leukemia.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" } ]

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Recovery

[ { "sample": "Serum", "range": "95 - 100 %", "average": "= 98" }, { "sample": "EDTA Plasma", "range": "91 - 97 %", "average": "= 93" }, { "sample": "Cell culture media", "range": "89 - 97 %", "average": "= 93" }, { "sample": "Heparin Plasma", "range": "87 - 97 %", "average": "= 92" }, { "sample": "Citrate plasma", "range": "93 - 105 %", "average": "= 98" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD127 also known as IL-7R alpha or IL-7Rα is a protein found on the surface of cells involved in key immune functions. The CD127 protein has an approximate mass of 55-60 kDa. It is a component of the interleukin-7 receptor which also includes the common gamma chain. You can find CD127 expressed on various immune cells like T cells B cells and dendritic cells. As a marker CD127 is an important indicator of T cell maturity and function. Researchers often target CD127 in studies using antibodies in assay formats like ELISA to measure its expression level and function.

Biological function summary

CD127 plays an important role in regulating lymphocyte development and survival. It is part of the IL-7R complex partnering with IL-7 for signal transduction that promotes cell proliferation and survival. This interaction is essential for the development and homeostasis of T cells and B cells. In the absence of proper CD127 signaling lymphocytes may suffer from poor survival impacting overall immune competence. The IL-7-CD127 interaction informs researchers about mechanisms of immune modulation and resilience.

Pathways

CD127 participates in the JAK/STAT signaling pathway which is pivotal for transmitting information from extracellular signals to the cell nucleus influencing gene expression. This pathway plays a vital role in immune cell response. When IL-7 binds to the IL-7R complex on CD127 it initiates a cascade that involves proteins like JAK1 and STAT5. These proteins mediate responses necessary for cell proliferation and differentiation within immune system networks.

CD127 has implications in conditions like severe combined immunodeficiency (SCID) and autoimmune diseases. In SCID mutations or defects in CD127 cause impaired T cell development leading to compromised immune function. Additionally CD127 dysregulation can contribute to autoimmune disorders by affecting T regulatory cells creating an imbalance in immune response. Ocrevus a monoclonal antibody therapy used for multiple sclerosis indirectly relates to CD127 by targeting the B cells which rely on IL-7R signaling mechanisms for survival and function. Researchers continue to investigate CD127 to better understand its role in immune-related diseases.

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Product protocols

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Target data

Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).

See full target information IL7R

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human CD127 ELISA Kit

Sandwich ELISA - Human CD127 ELISA Kit (AB270880)

Sandwich ELISA - Human CD127 ELISA Kit (AB270880)

Sandwich ELISA - Human CD127 ELISA Kit (AB270880)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise