AB239427

Human CD13 ELISA Kit

Recombinant
SimpleStep
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(1 Publication)

Human CD13 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD13 with a sensitivity of 25.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD13, APN, PEPN, ANPEP, Aminopeptidase N, AP-N, hAPN, Alanyl aminopeptidase, Aminopeptidase M, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, gp150, AP-M

5 Images

Sandwich ELISA - Human CD13 ELISA Kit (AB239427)

sELISA

Supplier Data

Sandwich ELISA - Human CD13 ELISA Kit (AB239427)

Representative image of Human CD13 ELISA Kit (ab239427)

Components shown from left to right :

- Sample diluent NS

- 10X Human CD13 Capture Antibody

- 10X Human CD13 Detector Antibody

- Human CD13 Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Stop Solution

- TMB solution

- 10X Wash Buffer PT (ab206977)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

sELISA

Unknown

Sandwich ELISA - Human CD13 ELISA Kit (AB239427)

Example of human CD13 standard curve in Sample Diluent NS + Enhancer. The CD13 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Human CD13 ELISA Kit (AB239427)

Interpolated concentrations of native CD13 in human serum and plasma samples. The concentrations of CD13 were measured in duplicates, interpolated from the CD13 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 160, plasma (citrate) 1 : 80, plasma (heparin) 1 : 80, and plasma (EDTA) 1 : 80. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD13 concentration was determined to be 724.9 ng/mL in serum, 658.0 ng/mL in plasma (citrate), 651.2 ng/ml in plasma (heparin), and 618.4 ng/mL in plasma (EDTA).

sELISA

Unknown

Sandwich ELISA - Human CD13 ELISA Kit (AB239427)

Interpolated concentrations of native CD13 in human HUVEC cell culture supernatant samples. The concentrations of CD13 were measured in duplicates, interpolated from the CD13 standard curves and corrected for sample dilution. Undiluted samples are as follows : HUVEC supernatant 1 : 40. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean, dilution factor corrected CD13 concentration was determined to be 366.5 ng/ml in HUVEC supernatant.

sELISA

Unknown

Sandwich ELISA - Human CD13 ELISA Kit (AB239427)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD13 concentration was determined to be 1245.6 ng/mL with a range of 1006.8 - 1765.3 ng/mL.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 25.6 pg/mL

Range

219 - 14000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human CD13 ELISA Kit (ab239427) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD13 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD13 with 25.6 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": null, "average": "= 90" }, { "sample": "Serum", "range": null, "average": "= 110" }, { "sample": "EDTA Plasma", "range": null, "average": "= 90" }, { "sample": "Heparin Plasma", "range": null, "average": "= 107" }, { "sample": "Citrate plasma", "range": null, "average": "= 83" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab239427-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB239427-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB239427-CMP08", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB239427-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB239427-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB239427-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB239427-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB239427-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD13 Capture Antibody", "number":"AB239427-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CD13 Lyophilized Recombinant Protein", "number":"AB239427-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB239427-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD13 Detector Antibody", "number":"AB239427-CMP10", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD13 also known as aminopeptidase N (ANPEP) is a transmembrane protein with a molecular weight of approximately 150 kDa. It functions as a zinc-dependent metalloenzyme which cleaves N-terminal amino acids from peptides and proteins. CD13 protein is expressed on various cell types including myeloid cells epithelial cells endothelial cells and fibroblasts. Its presence is significantly observed in the brush border of the small intestinal mucosa and the renal proximal tubule. Researchers can study CD13 using anti-CD13 antibodies and CD13 ELISA kits.

Biological function summary

CD13 regulates peptide-mediated signaling and controls the maturation and catabolism of bioactive peptides. The enzymatic function of CD13 influences processes such as cell proliferation motility and angiogenesis. It does not operate as part of a larger complex but its activity modulates several cellular and systemic functions. CD13's role in these biological processes highlights its importance in modulating local and systemic peptide pools which contributes to its diverse physiological effects.

Pathways

CD13 plays significant roles in the renin-angiotensin system and the regulation of inflammatory responses. In the renin-angiotensin system CD13 modulates the activity of angiotensin influencing blood pressure and fluid balance. Through its enzymatic activity CD13 interacts with proteins such as ACE another critical player in this pathway. In inflammation CD13 regulates the availability of chemotactic peptides affecting leukocyte migration and adhesion. The proteins it works with in inflammatory pathways include cytokines which CD13 indirectly modulates by altering chemokine activity.

Aberrant CD13 expression and activity are implicated in cancer and inflammatory diseases. In cancer overexpression of CD13 correlates with tumor growth and metastasis particularly in cancers like renal cell carcinoma and prostate cancer. It can confer an increased malignant phenotype by promoting angiogenesis and immune evasion. In inflammatory disorders dysregulation of CD13 exacerbates diseases such as rheumatoid arthritis through its impact on peptide-mediated signaling and immune cell movement. Its connection with inflammatory cytokines like IL-8 indicates its important role in mediating inflammatory responses.

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Product protocols

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Target data

Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization. May have a role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 and regulating its activity (By similarity).. (Microbial infection) Acts as a receptor for human coronavirus 229E/HCoV-229E. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein.. (Microbial infection) Mediates as well Human cytomegalovirus (HCMV) infection.

See full target information ANPEP

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinical epigenetics 15:180 PubMed37941056

2023

Tazemetostat decreases β-catenin and CD13 protein expression in HEPG-2 and Hepatitis B virus-transfected HEPG-2 with decreased cell viability.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed N Amin,Yousra M El-Far,Mohammed El-Mowafy,Abdelaziz Elgaml

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human CD13 ELISA Kit

Sandwich ELISA - Human CD13 ELISA Kit (AB239427)

Sandwich ELISA - Human CD13 ELISA Kit (AB239427)

Sandwich ELISA - Human CD13 ELISA Kit (AB239427)

Sandwich ELISA - Human CD13 ELISA Kit (AB239427)

Sandwich ELISA - Human CD13 ELISA Kit (AB239427)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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