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AB46135

Human CD130 ELISA Kit (GP130)

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(1 Publication)

Human CD130 ELISA Kit (GP130) is a Sandwich (quantitative) ELISA for the measurement of Human CD130 (GP130) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD130, Interleukin-6 receptor subunit beta, IL-6 receptor subunit beta, IL-6R subunit beta, IL-6R-beta, IL-6RB, CDw130, Interleukin-6 signal transducer, Membrane glycoprotein 130, Oncostatin-M receptor subunit alpha, gp130, IL6ST

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Sandwich ELISA - Human CD130 ELISA Kit (GP130) (AB46135)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD130 ELISA Kit (GP130) (AB46135)

Representative Standard Curve using ab46135

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 15 pg/mL

Range

56.25 - 1800 pg/mL

Assay time

1h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's CD130 (GP130) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD130 in Human serum, plasma, buffered solutions and supernatants.

A monoclonal antibody specific for CD130 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD130 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD130 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD130 present in the samples.

This kit will recognize both endogenous and recombinant Human CD130.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 16, "mean": "1782 pg/mL", "standardDeviation": "26.64", "coefficientOfVariability": "1.49" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 16, "mean": "247 pg/mL", "standardDeviation": "11.99", "coefficientOfVariability": "4.85" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 8, "mean": "1751 pg/mL", "standardDeviation": "17.61", "coefficientOfVariability": "1" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 8, "mean": "264.3 pg/mL", "standardDeviation": "8.67", "coefficientOfVariability": "3.27" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "= 97" } ]
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What's included?

{ "values": { "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46135-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46135-CMP04" }, { "size":"2 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46135-CMP07" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46135-CMP11" }, { "size":"4 x 1 Vial", "name":"Control", "number":"AB46135-CMP08" }, { "size":"2 x 400 µL", "name":"Biotinylated anti-CD130", "number":"AB46135-CMP01" }, { "size":"2 x 1 Unit", "name":"CD130 Microplate (12 x 8 well strips)", "number":"AB46135-CMP09" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46135-CMP06" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46135-CMP02" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46135-CMP05" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46135-CMP03" }, { "size":"4 x 1 Vial", "name":"CD130 Standard (lyophilized)", "number":"AB46135-CMP10" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46135-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46135-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CD130 Microplate (12 x 8 well strips)", "number":"AB46135-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46135-CMP07", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46135-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46135-CMP06", "productcode":"" }, { "size":"1 x 400 µL", "name":"Biotinylated anti-CD130", "number":"AB46135-CMP01", "productcode":"" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46135-CMP05", "productcode":"" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46135-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"CD130 Standard (lyophilized)", "number":"AB46135-CMP10", "productcode":"" }, { "size":"1 x 7.5 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46135-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Control", "number":"AB46135-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD130 also known as gp130 or the IL-6 signal transducer (IL6ST) is a protein weighing around 130 kDa. This receptor is encoded by the IL6ST gene and widely expressed on the surface of cells across multiple tissues. CD130 functions mechanically as a signal transducing receptor facilitating the assembly of receptor complexes necessary for the initiation of signal transduction. It plays a major role in cytokine receptor signaling particularly interacting with interleukin-6 (IL-6) family cytokines.
Biological function summary

CD130 functions as part of the high-affinity receptor complex for certain cytokines. It partners with other receptors such as CD126 to form a complex that initiates signaling pathways after ligand binding contributing to a range of immune responses. Its role is thorough across many cellular processes including differentiation proliferation and apoptosis. In addition to immune signaling CD130 is involved in several growth factor signaling pathways.

Pathways

CD130 serves a significant role in signal transduction particularly in the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway. It works in concert with related proteins like JAK1 and STAT3 to transmit signals from extracellular cytokines to the nucleus influencing gene expression. Additionally CD130 can also be found active in the MAPK/ERK pathway which is important for cellular response to growth signals.

CD130 plays a connection especially to inflammatory conditions and certain cancers. Aberrant signaling involving CD130 has been linked to disorders like rheumatoid arthritis where it contributes to the inflammation process. Additionally it relates to multiple myeloma a cancer where abnormal CD130 interactions with proteins like IL-6 may enhance cell survival and proliferation presenting potential targets for therapeutic intervention.
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Product protocols

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Target data

Signal-transducing molecule (PubMed : 2261637). The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize IL6ST for initiating signal transmission. Binding of IL6 to IL6R induces IL6ST homodimerization and formation of a high-affinity receptor complex, which activates the intracellular JAK-MAPK and JAK-STAT3 signaling pathways (PubMed : 19915009, PubMed : 2261637, PubMed : 23294003). That causes phosphorylation of IL6ST tyrosine residues which in turn activates STAT3 (PubMed : 19915009, PubMed : 23294003, PubMed : 25731159). In parallel, the IL6 signaling pathway induces the expression of two cytokine receptor signaling inhibitors, SOCS1 and SOCS3, which inhibit JAK and terminate the activity of the IL6 signaling pathway as a negative feedback loop (By similarity). Also activates the yes-associated protein 1 (YAP) and NOTCH pathways to control inflammation-induced epithelial regeneration, independently of STAT3 (By similarity). Acts as a receptor for the neuroprotective peptide humanin as part of a complex with IL27RA/WSX1 and CNTFR (PubMed : 19386761). Mediates signals which regulate immune response, hematopoiesis, pain control and bone metabolism (By similarity). Has a role in embryonic development (By similarity). Essential for survival of motor and sensory neurons and for differentiation of astrocytes (By similarity). Required for expression of TRPA1 in nociceptive neurons (By similarity). Required for the maintenance of PTH1R expression in the osteoblast lineage and for the stimulation of PTH-induced osteoblast differentiation (By similarity). Required for normal trabecular bone mass and cortical bone composition (By similarity).. Isoform 2. Binds to the soluble IL6 : sIL6R complex (hyper-IL6), thereby blocking IL6 trans-signaling. Inhibits sIL6R-dependent acute phase response (PubMed : 11121117, PubMed : 21990364, PubMed : 30279168). Also blocks IL11 cluster signaling through IL11R (PubMed : 30279168).
See full target information IL6ST
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in physiology 12:722528 PubMed34707507

2021

Soluble Interleukin-6 Receptor Regulates Interleukin-6-Dependent Vascular Remodeling in Long-Distance Runners.

Applications

Unspecified application

Species

Unspecified reactive species

Paulina Villar-Fincheira,Aaron J Paredes,Tomás Hernández-Díaz,Ignacio Norambuena-Soto,Nicole Cancino-Arenas,Fernanda Sanhueza-Olivares,Felipe Contreras-Briceño,Jorge Mandiola,Nicole Bruneau,Lorena García,María Paz Ocaranza,Rodrigo Troncoso,Luigi Gabrielli,Mario Chiong
View all publications
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