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Human CD130 ELISA Kit (GP130) is a Sandwich (quantitative) ELISA kit for the measurement of Human CD130 (GP130) in Human in Plasma, Cell culture supernatant, Serum samples.

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Sandwich ELISA - Human CD130 ELISA Kit (GP130) (AB46135), expandable thumbnail

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Key facts

Detection method
Colorimetric
Sample types
Plasma, Cell culture supernatant, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
56.25 - 1800 pg/mL
Assay time
1h 45m
Sensitivity
< 15 pg/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Signal-transducing molecule (PubMed:2261637). The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize IL6ST for initiating signal transmission. Binding of IL6 to IL6R induces IL6ST homodimerization and formation of a high-affinity receptor complex, which activates the intracellular JAK-MAPK and JAK-STAT3 signaling pathways (PubMed:19915009, PubMed:2261637, PubMed:23294003). That causes phosphorylation of IL6ST tyrosine residues which in turn activates STAT3 (PubMed:19915009, PubMed:23294003, PubMed:25731159). In parallel, the IL6 signaling pathway induces the expression of two cytokine receptor signaling inhibitors, SOCS1 and SOCS3, which inhibit JAK and terminate the activity of the IL6 signaling pathway as a negative feedback loop (By similarity). Also activates the yes-associated protein 1 (YAP) and NOTCH pathways to control inflammation-induced epithelial regeneration, independently of STAT3 (By similarity). Acts as a receptor for the neuroprotective peptide humanin as part of a complex with IL27RA/WSX1 and CNTFR (PubMed:19386761). Mediates signals which regulate immune response, hematopoiesis, pain control and bone metabolism (By similarity). Has a role in embryonic development (By similarity). Essential for survival of motor and sensory neurons and for differentiation of astrocytes (By similarity). Required for expression of TRPA1 in nociceptive neurons (By similarity). Required for the maintenance of PTH1R expression in the osteoblast lineage and for the stimulation of PTH-induced osteoblast differentiation (By similarity). Required for normal trabecular bone mass and cortical bone composition (By similarity). Isoform 2. Binds to the soluble IL6:sIL6R complex (hyper-IL6), thereby blocking IL6 trans-signaling. Inhibits sIL6R-dependent acute phase response (PubMed:11121117, PubMed:21990364, PubMed:30279168). Also blocks IL11 cluster signaling through IL11R (PubMed:30279168).

Alternative names

CD130, Interleukin-6 receptor subunit beta, IL-6 receptor subunit beta, IL-6R subunit beta, IL-6R-beta, IL-6RB, CDw130, Interleukin-6 signal transducer, Membrane glycoprotein 130, Oncostatin-M receptor subunit alpha, gp130, IL6ST

What's included?

2 x 96 Tests
Components
10X Standard Diluent Buffer
2 x 25 mL
200X Wash Buffer
2 x 10 mL
Biotinylated Antibody Diluent
1 x 13 mL
Biotinylated anti-CD130
2 x 400 µL
CD130 Microplate (12 x 8 well strips)
2 x 1 Unit
CD130 Standard (lyophilized)
4 x 1 Vial
Chromogen TMB Substrate Solution
1 x 24 mL
Control
4 x 1 Vial
HRP Diluent
1 x 23 mL
Stop Reagent
2 x 11 mL
Streptavidin-HRP
4 x 5 µL

Recommended products

Human CD130 ELISA Kit (GP130) is a Sandwich (quantitative) ELISA kit for the measurement of Human CD130 (GP130) in Human in Plasma, Cell culture supernatant, Serum samples.

Key facts

Detection method
Colorimetric
Sample types
Plasma, Cell culture supernatant, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
56.25 - 1800 pg/mL
Assay time
1h 45m
Assay Platform
Microplate
Sensitivity
< 15 pg/mL
General recovery
97%

Precision

Intra assay

Sample
Sample A
n
8
mean
1751 pg/mL
SD
17.61
C.V.
1
Sample
Sample B
n
8
mean
264.3 pg/mL
SD
8.67
C.V.
3.27

Inter assay

Sample
Sample A
n
16
mean
1782 pg/mL
SD
26.64
C.V.
1.49
Sample
Sample B
n
16
mean
247 pg/mL
SD
11.99
C.V.
4.85

Recovery

Sample specific recovery

Sample type
Serum
Average %
= 97
Range

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

Abcam's CD130 (GP130) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD130 in Human serum, plasma, buffered solutions and supernatants.

A monoclonal antibody specific for CD130 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD130 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD130 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD130 present in the samples.

This kit will recognize both endogenous and recombinant Human CD130.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD130 also known as gp130 or the IL-6 signal transducer (IL6ST) is a protein weighing around 130 kDa. This receptor is encoded by the IL6ST gene and widely expressed on the surface of cells across multiple tissues. CD130 functions mechanically as a signal transducing receptor facilitating the assembly of receptor complexes necessary for the initiation of signal transduction. It plays a major role in cytokine receptor signaling particularly interacting with interleukin-6 (IL-6) family cytokines.

Biological function summary

CD130 functions as part of the high-affinity receptor complex for certain cytokines. It partners with other receptors such as CD126 to form a complex that initiates signaling pathways after ligand binding contributing to a range of immune responses. Its role is thorough across many cellular processes including differentiation proliferation and apoptosis. In addition to immune signaling CD130 is involved in several growth factor signaling pathways.

Pathways

CD130 serves a significant role in signal transduction particularly in the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway. It works in concert with related proteins like JAK1 and STAT3 to transmit signals from extracellular cytokines to the nucleus influencing gene expression. Additionally CD130 can also be found active in the MAPK/ERK pathway which is important for cellular response to growth signals.

Associated diseases and disorders

CD130 plays a connection especially to inflammatory conditions and certain cancers. Aberrant signaling involving CD130 has been linked to disorders like rheumatoid arthritis where it contributes to the inflammation process. Additionally it relates to multiple myeloma a cancer where abnormal CD130 interactions with proteins like IL-6 may enhance cell survival and proliferation presenting potential targets for therapeutic intervention.

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1 product image

  • Sandwich ELISA - Human CD130 ELISA Kit (GP130) (ab46135), expandable thumbnail

    Sandwich ELISA - Human CD130 ELISA Kit (GP130) (ab46135)

    Representative Standard Curve using ab46135

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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