JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB45523

Human CD14 ELISA Kit

Be the first to review this product! Submit a review

|

(1 Publication)

Human CD14 ELISA Kit is a Sandwich ELISA for the measurement of Human CD14 in Human in Biofluids, Cell Culture Media, Cell/Tissue Extracts samples.

View Alternative Names

CD14, Monocyte differentiation antigen CD14, My23 antigen, Myeloid cell-specific leucine-rich glycoprotein

2 Images
Sandwich ELISA - Human CD14 ELISA Kit (AB45523)
  • sELISA

Lab

Sandwich ELISA - Human CD14 ELISA Kit (AB45523)

Sandwich ELISA -HuCD14 ELISA Kit.

Colorimetric standard curve : mean of duplicates (+/-SD) with background readings subtracted

Sandwich ELISA - Human CD14 ELISA Kit (AB45523)
  • sELISA

Lab

Sandwich ELISA - Human CD14 ELISA Kit (AB45523)

Sandwich ELISA - HuCD14 Elisa Kit.

hu CD14 measured in biologicals showing quantity (ng) per mL of tested sample

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Milk, Serum, Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 6 ng/mL

Range

6.89 - 220 ng/mL

Assay time

3h 45m

Assay Platform

Microplate

style
left-column

Complementary Products

Assay Kits

AB181421

Human TNF alpha ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

  • 5
  • 4
Reagents, Controls & Accessories

AB206978

Pre-Coated 96 Well Microplate (12 x 8 well strips) SimpleStep ELISA®

SimpleStep

ELISA - Pre-Coated 96 Well Microplate (12 x 8 well strips) SimpleStep ELISA® (AB206978)

  • 0
  • 0
Reagents, Controls & Accessories

AB203359

Pre-coated 384 well Microplate SimpleStep ELISA®

SimpleStep

ELISA - Pre-coated 384 well Microplate SimpleStep ELISA® (AB203359)

  • 5
  • 1
Assay Kits

AB174443

Human IFN gamma ELISA Kit

SimpleStep

ELISA - Human IFN gamma ELISA Kit (AB174443)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's CD14 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD14 in Human serum, plasma, buffered solutions or cell culture media.

A monoclonal antibody specific for CD14 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD14 concentrations and unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD14 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD14 present in the samples.

This kit will recognize both endogenous and recombinant Human CD14.

Get higher sensitivity in only 90 minutes with Human CD14 ELISA Kit (ab208983) from our SimpleStep ELISA® range.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 8, "mean": "210.5 ng/mL", "standardDeviation": "1.55", "coefficientOfVariability": "0.73" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 8, "mean": "24 ng/mL", "standardDeviation": "2", "coefficientOfVariability": "8.3" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 8, "mean": "210.5 ng/mL", "standardDeviation": "2.6", "coefficientOfVariability": "1.23" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 8, "mean": "23.4 ng/mL", "standardDeviation": "1.14", "coefficientOfVariability": "4.6" } ]
style
left-column

What's included?

{ "values": { "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab45523-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB45523-CMP03" }, { "size":"4 x 1 Vial", "name":"CD14 Standard (lyophilized)", "number":"AB45523-CMP09" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB45523-CMP01" }, { "size":"2 x 1 Unit", "name":"CD14 Microplate (12 x 8 well strips)", "number":"AB45523-CMP08" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB45523-CMP02" }, { "size":"2 x 0.3 mL", "name":"Biotinylated anti-CD14", "number":"AB45523-CMP07" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB45523-CMP04" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB45523-CMP05" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB45523-CMP10" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB45523-CMP06" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab45523-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB45523-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"CD14 Standard (lyophilized)", "number":"AB45523-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB45523-CMP06", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB45523-CMP02", "productcode":"" }, { "size":"1 x 7 mL", "name":"Biotinylated Antibody Diluent", "number":"AB45523-CMP01", "productcode":"" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB45523-CMP04", "productcode":"" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB45523-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CD14 Microplate (12 x 8 well strips)", "number":"AB45523-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB45523-CMP05", "productcode":"" }, { "size":"1 x 0.3 mL", "name":"Biotinylated anti-CD14", "number":"AB45523-CMP07", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD14 also known as cluster of differentiation 14 is a glycoprotein with a molecular mass of approximately 55 kDa. This protein is expressed mainly on the surface of macrophages monocytes and to a lesser extent on neutrophils. CD14 exists in two forms: membrane-bound (mCD14) and soluble (sCD14). It functions as a co-receptor along with TLR4 (Toll-like receptor 4) and MD-2 for the detection of bacterial lipopolysaccharide (LPS) which is an important component of the outer membrane of Gram-negative bacteria.
Biological function summary

This glycoprotein plays a significant role in the innate immune system by recognizing pathogen-associated molecular patterns (PAMPs). It is part of a receptor complex where CD14 acts alongside LPS binding protein (LPS-R) TLR4 and MD-2 to facilitate pro-inflammatory signaling in response to microbial invasion. By binding to LPS CD14 initiates and amplifies the immune response leading to the production of cytokines and the recruitment of other immune cells to the site of infection.

Pathways

CD14 interacts with the toll-like receptor signaling pathway notably involving TLR4. Upon LPS detection CD14 assists in the activation of TLR4 triggering downstream signaling cascades such as NF-κB and MAPK pathways. These pathways result in the transcription of inflammatory cytokines and are essential for the host defense mechanism. The protein complex involving CD14 further interacts with adaptor proteins like MyD88 and TRIF facilitating signal propagation and the immune system's ability to respond to pathogens.

Researchers have linked CD14 to sepsis and atherosclerosis. High levels of soluble CD14 (sCD14) are often observed in patients with sepsis indicating its participation in excessive systemic inflammation. In atherosclerosis CD14's role in recognizing LPS contributes to chronic inflammation and the development of plaques in arteries. Through these diseases CD14 connects with TLR4 emphasizing its impact on inflammatory responses and its potential as a therapeutic target.
style
left-column

Product protocols

style
left-column

Target data

Coreceptor for bacterial lipopolysaccharide (PubMed : 1698311, PubMed : 23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed : 20133493, PubMed : 22265692, PubMed : 23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 8612135). Acts as a coreceptor for TLR2 : TLR6 heterodimer in response to diacylated lipopeptides and for TLR2 : TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed : 16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed : 23880187).
See full target information CD14
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Mediators of inflammation 2014:198413 PubMed24808634

2014

Circulating biomarkers of immune activation distinguish viral suppression from nonsuppression in HAART-treated patients with advanced HIV-1 subtype C infection.

Applications

Unspecified application

Species

Unspecified reactive species

Glen Malherbe,Helen C Steel,Sharon Cassol,Tulio de Oliveira,Christopher J Seebregts,Ronald Anderson,Edana Cassol,Theresa M Rossouw
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com