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AB267623

Human CD157 ELISA kit

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Human CD157 ELISA kit is a Sandwich ELISA for the measurement of Human CD157 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD157, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2, ADP-ribosyl cyclase 2, Bone marrow stromal cell antigen 1, Cyclic ADP-ribose hydrolase 2, BST-1, cADPR hydrolase 2, BST1

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Sandwich ELISA - Human CD157 ELISA kit (AB267623)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD157 ELISA kit (AB267623)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.06 ng/mL

Range

0.051 - 12.5 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CD157 ELISA Kit is designed for the quantitative determination of CD157 in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for human CD157 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD157 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CD157 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD157 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit shows no cross-reactivity with the following cytokines tested: Human AMIGO, Aminopeptidase LRAP (ERAP2), Amnionless, Arylsulfatase A, Bcl-w, CD109, CD34, CD83, CLEC-1, CLEC10A (CD301), CMG-2, CREG, Cystatin SN, Cytokeratin-8, Dectin-1 (CLEC7A), Desmocollin-3, Endoglycan, Galectin-4, HAPLN1, Jagged 1, Langerin (CD207), Lumican, Matriptase (ST14), MEP1B, Nectin-3, OX40 (TNFRSF4), OX40 Ligand (TNFSF4), p27, Pappalysin-2, Plexin B3, Plexin D1, proGRP, PSA-total, Reg1B, RGM-A, ROBO2, Spinesin, TWEAK R (TNFRSF12), ULBP-3.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "124 - 138 %", "average": "= 130.9" }, { "sample": "Plasma", "range": "113 - 145 %", "average": "= 129.8" }, { "sample": "Cell culture media", "range": "112 - 140 %", "average": "= 123.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267623-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB267623-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267623-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267623-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human CD157 coated Microplate (12 x 8 wells)", "number":"AB267623-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human CD157 Detection Antibody", "number":"AB267623-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CD157 Standard (Lyophilized)", "number":"AB267623-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267623-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin Concentrate", "number":"AB267623-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bone marrow stromal cell antigen 1 (BST-1) also known as CD157 is a glycosylphosphatidylinositol-anchored protein with a molecular weight of approximately 38-42 kDa. You find BST-1 expression on the surface of various cell types especially within bone marrow stromal cells and cells of the immune system such as monocytes and macrophages. This protein functions as an ecto-enzyme with ADP-ribosyl cyclase activity and is part of the NADase enzyme family. Its mechanical role involves metabolizing extracellular NAD+ influencing cellular calcium signaling and energy metabolism.
Biological function summary

BST-1 contributes to the regulation of cellular interactions and immune responses. It participates in the organization of leukocyte trafficking and the formation of signal transduction cascades. BST-1 operates as a single protein but interacts with other membrane proteins to coordinate cellular communication. You can observe its function in processes like cell adhesion and migration impacting the immune surveillance and response systems.

Pathways

BST-1 plays a role in immune signaling and calcium-signaling pathways. In the immune system pathway it associates with molecules such as integrins to modulate leukocyte adhesion and transmigration. The calcium-signaling pathway is another key area where BST-1 interacts with intracellular proteins influencing various cellular targets and affecting metabolic processes critical for cell survival and proliferation.

Scientists have linked BST-1 to various conditions such as leukemia and chronic inflammation. Its expression levels often change in these diseases emphasizing its potential role in their progression. Researchers also explore the connection between BST-1 and other proteins like CD38 in the context of leukemia where both proteins might participate in cancer cell proliferation and survival. Understanding BST-1 in disease contexts provides insights into disease mechanisms and potential therapeutic strategies.
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Product protocols

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Target data

Catalyzes both the synthesis of cyclic ADP-beta-D-ribose (cADPR) from NAD(+), and its hydrolysis to ADP-D-ribose (ADPR) (PubMed : 7805847). Cyclic ADPR is known to serve as an endogenous second messenger that elicits calcium release from intracellular stores, and thus regulates the mobilization of intracellular calcium (Probable). May be involved in pre-B-cell growth (Probable).
See full target information BST1
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websiteProtocolBooklet
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Product promise

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