Human CD16 ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human CD16 with a sensitivity of 5.717 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 19.531 - 1250 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 5.717 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Receptor for the invariable Fc fragment of immunoglobulin gamma (IgG). Optimally activated upon binding of clustered antigen-IgG complexes displayed on cell surfaces, triggers lysis of antibody-coated cells, a process known as antibody-dependent cellular cytotoxicity (ADCC). Does not bind free monomeric IgG, thus avoiding inappropriate effector cell activation in the absence of antigenic trigger (PubMed:11711607, PubMed:21768335, PubMed:22023369, PubMed:24412922, PubMed:25786175, PubMed:25816339, PubMed:28652325, PubMed:8609432, PubMed:9242542). Mediates IgG effector functions on natural killer (NK) cells. Binds antigen-IgG complexes generated upon infection and triggers NK cell-dependent cytokine production and degranulation to limit viral load and propagation. Involved in the generation of memory-like adaptive NK cells capable to produce high amounts of IFNG and to efficiently eliminate virus-infected cells via ADCC (PubMed:24412922, PubMed:25786175). Regulates NK cell survival and proliferation, in particular by preventing NK cell progenitor apoptosis (PubMed:29967280, PubMed:9916693). Fc-binding subunit that associates with CD247 and/or FCER1G adapters to form functional signaling complexes. Following the engagement of antigen-IgG complexes, triggers phosphorylation of immunoreceptor tyrosine-based activation motif (ITAM)-containing adapters with subsequent activation of phosphatidylinositol 3-kinase signaling and sustained elevation of intracellular calcium that ultimately drive NK cell activation. The ITAM-dependent signaling coupled to receptor phosphorylation by PKC mediates robust intracellular calcium flux that leads to production of pro-inflammatory cytokines, whereas in the absence of receptor phosphorylation it mainly activates phosphatidylinositol 3-kinase signaling leading to cell degranulation (PubMed:1825220, PubMed:23024279, PubMed:2532305). Costimulates NK cells and trigger lysis of target cells independently of IgG binding (PubMed:10318937, PubMed:23006327). Mediates the antitumor activities of therapeutic antibodies. Upon ligation on monocytes triggers TNFA-dependent ADCC of IgG-coated tumor cells (PubMed:27670158). Mediates enhanced ADCC in response to afucosylated IgGs (PubMed:34485821). (Microbial infection) Involved in Dengue virus pathogenesis via antibody-dependent enhancement (ADE) mechanism. Secondary infection with Dengue virus triggers elevated levels of afucosylated non-neutralizing IgG1s with reactivity to viral envelope/E protein. Viral antigen-IgG1 complexes bind with high affinity to FCGR3A, facilitating virus entry in myeloid cells and subsequent viral replication.
Additional Targets
FCGR3B
Alternative names
CD16a, CD16A, FCG3, FCGR3, IGFR3, FCGR3A, Low affinity immunoglobulin gamma Fc region receptor III-A, IgG Fc receptor III-A, CD16-II, CD16a antigen, Fc-gamma RIII-alpha, FcR-10, IgG Fc receptor III-2, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcgammaRIIIA
What's included?
Recommended products
Human CD16 ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human CD16 with a sensitivity of 5.717 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 19.531 - 1250 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 5.717 pg/mL
- General recovery
- 99%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 4.1 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. 2.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Tissue Extracts | Average % = 99 | Range 97 - 100 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human CD16 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD16 protein in Tissue extracts. Quantitate Human CD16 with 5.717 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD16 often known as Fc gamma receptor III (FcγRIII) comes in two isoforms: CD16a and CD16b. CD16a is a transmembrane protein with a mass of about 50-65 kDa while CD16b a glycosylphosphatidylinositol (GPI)-anchored receptor holds a mass near 48-61 kDa. CD16 is expressed on natural killer (NK) cells monocytes macrophages and neutrophils depending on the variant. It serves as a low-affinity receptor for the Fc portion of IgG antibodies mediating essential immune functions like antibody-dependent cellular cytotoxicity (ADCC).
- Biological function summary
CD16 plays an important role in immune response regulation and is part of the larger IgG receptor family. As a member of the receptor complex CD16 enables effector cells to identify and eliminate IgG-bound pathogens or diseased cells. The differential expression of CD16a and CD16b provides distinct roles in immune signaling. CD16a expressed on NK cells is involved in ADCC by enabling these cells to recognize and destroy antibody-coated target cells. CD16b predominantly found on neutrophils influences the phagocytosis process and aids in inflammatory response modulation.
- Pathways
CD16 integrates into significant immune response pathways. In the ADCC pathway CD16a interacts with other proteins like FcεRI gamma-chain to trigger cytotoxic activities in NK cells. The CD16b isoform participates in neutrophil-mediated immune responses connecting to downstream signaling molecules that modulate inflammation and pathogen clearance. These interactions ensure an effective immune response and maintain homeostasis by removing pathogenic threats.
- Associated diseases and disorders
CD16 plays vital roles in immune-related conditions like autoimmune diseases and chronic inflammation. Aberrant CD16 expression or function is associated with autoimmune conditions such as rheumatoid arthritis where altered CD16a and CD16b expressions contribute to the pathology. Furthermore CD16 links to systemic lupus erythematosus (SLE) interacting with proteins like complement factors C3 and C4 influencing disease progression and severity. Understanding CD16's role offers insights into these disorders and informs potential therapeutic strategies.
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2 product images
Sandwich ELISA - Human CD16 ELISA Kit- Intracellular (ab316910)
Example of human CD16 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human CD16 ELISA Kit- Intracellular (ab316910)
Interpolated concentration of native CD16 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: human lung extract 50 µg/mL, human liver extract 150 µg/mL, and human spleen extract 10 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
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