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AB274394

Human CD163 ELISA Kit

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(4 Publications)

Human CD163 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD163 with a sensitivity of 28.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD163, M130, Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor

8 Images
ELISA - Human CD163 ELISA Kit (AB274394)
  • ELISA

Supplier Data

ELISA - Human CD163 ELISA Kit (AB274394)
Sandwich ELISA - Human CD163 ELISA Kit (AB274394)
  • sELISA

Lab

Sandwich ELISA - Human CD163 ELISA Kit (AB274394)

Interpolated concentration of native CD163 was measured in duplicate at different sample concentrations in 96-well vs 384-well plates. Undiluted samples are 1 : 100 serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent 25BS.

Sandwich ELISA - Human CD163 ELISA Kit (AB274394)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD163 ELISA Kit (AB274394)

Interpolated concentrations of native CD163 in stimulated human PBMC cell culture supernatant samples.

PBMC samples were stimulated for 46 hours with 1.5% PHA-M. The concentrations of CD163 were measured in duplicates, interpolated from the CD163 standard curves and corrected for sample dilution. Undiluted samples are as follows : stimulated PBMC supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD163 concentration was determined to be 22 ng/mL in stimulated PBMC supernatant.

Sandwich ELISA - Human CD163 ELISA Kit (AB274394)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD163 ELISA Kit (AB274394)

Example of human CD163 standard curve in Sample Diluent 25BS.

The CD163 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CD163 ELISA Kit (AB274394)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD163 ELISA Kit (AB274394)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD163 concentration was determined to be 563 ng/mL with a range of 338 – 1,030 ng/mL.

Sandwich ELISA - Human CD163 ELISA Kit (AB274394)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD163 ELISA Kit (AB274394)

Interpolated concentrations of native CD163 in human serum and plasma samples.

The concentrations of CD163 were measured in duplicates, interpolated from the CD163 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1%, plasma (citrate) 1%, plasma (heparin) 1%, and plasma (EDTA) 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD163 concentration was determined to be 704 ng/mL in serum, 552 ng/mL in plasma (citrate), 545 ng/ml in plasma (heparin), and 515 ng/mL in plasma (EDTA).

Sandwich ELISA - Human CD163 ELISA Kit (AB274394)
  • sELISA

Lab

Sandwich ELISA - Human CD163 ELISA Kit (AB274394)

Example of human CD163 standard curve in 96-well vs 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CD163 ELISA Kit (AB274394)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD163 ELISA Kit (AB274394)

Human CD163 competitor curve comparison.

Standard Curve comparison between Human CD163 SimpleStep ELISA kit and traditional ELISA kit from leading competitor.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 28.2 pg/mL

Range

125 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CD163 ELISA Kit ab274394 is a rapid single-wash 90-min Sandwich ELISA to measure Human CD163 in cell culture supernatant, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 28.2 pg/mL.

How the assay works

Human CD163 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human CD163 ELISA Kit ab274394 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Human CD163 ELISA Kit only requires a maximum of 12.5µL sample

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-CD163 antibody [EPR22675-13] - BSA and Azide free (Capture) ab274441
- Anti-CD163 antibody [EPR22675-102] - BSA and Azide free (Detector) ab274441

CD163 is a scavenge receptor for the haptoglobin-hemoglobin complex. CD163 clears the complex in the liver, spleen and circulation. Soluble CD163 also plays a role in reducing inflammation, and dysregulation of CD163 is associated with some inflammatory conditions like multiple sclerosis, rheumatoid arthritis and diabetes mellitus.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "100 - 112 %", "average": "= 106" }, { "sample": "Serum", "range": "103 - 117 %", "average": "= 108" }, { "sample": "EDTA Plasma", "range": "105 - 120 %", "average": "= 111" }, { "sample": "Heparin Plasma", "range": "106 - 115 %", "average": "= 111" }, { "sample": "Citrate plasma", "range": "90 - 102 %", "average": "= 97" } ]
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What's included?

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab274394-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB274394-CMP06" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB274394-CMP08" }, { "size":"2 x 1 Vial", "name":"Human CD163 Lyophilized Recombinant Protein", "number":"AB274394-CMP11" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB274394-CMP10" }, { "size":"1 x 600 µL", "name":"10X Human CD163 Capture Antibody", "number":"AB274394-CMP02" }, { "size":"1 x 20 mL", "name":"Sample Diluent 25BS", "number":"AB274394-CMP03" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB274394-CMP09" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB274394-CMP07" }, { "size":"1 x 600 µL", "name":"10X Human CD163 Detector Antibody", "number":"AB274394-CMP01" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB274394-CMP13" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab274394-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB274394-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB274394-CMP12", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB274394-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB274394-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CD163 Lyophilized Recombinant Protein", "number":"AB274394-CMP11", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 25BS", "number":"AB274394-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD163 Capture Antibody", "number":"AB274394-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB274394-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD163 Detector Antibody", "number":"AB274394-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB274394-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB274394-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD163 known also as hemoglobin scavenger receptor is a membrane glycoprotein with a molecular weight of approximately 130 kDa. It is predominantly expressed by monocytes and macrophages which are types of immune cells. Located on the cell surface CD163 functions as a receptor that aids in the clearance of hemoglobin-haptoglobin complexes playing a critical role in maintaining iron homeostasis and protecting tissues from oxidative damage. CD163 is not limited to human cells only; researchers often study its function using animal models such as chimeric rodents and chimeric rats.
Biological function summary

CD163 participates in anti-inflammatory processes and is linked to immune modulation. It plays a pivotal role in the resolution of inflammation and promotes the removal of damaged cells and tissues. Through its involvement in the endocytic pathway CD163 interacts with various ligands facilitating interactions with other proteins. In the context of research CD163 functionality can be analyzed using techniques like CD163 IHC CD163 ELISA and CD163 stain in both human and mouse models.

Pathways

CD163 is integral to the inflammation and immune response pathways. It participates in the heme-oxygenase pathway which is essential in the breakdown of heme into biliverdin free iron and carbon monoxide. This pathway involvement is tightly linked with heme oxygenase-1 (HO-1) a cytoprotective enzyme that helps to mitigate inflammatory responses. The interaction with haptoglobin and related proteins provides further insight into its regulatory roles within the immune system.

CD163 is associated with conditions like atherosclerosis and acute inflammation disorders. Its elevated levels indicate macrophage activation observed in chronic inflammations such as cardiovascular diseases. In atherosclerosis CD163 expression correlates with the uptake of oxidized LDL connecting it to progression of the disease. Additionally soluble CD163 levels serve as a biomarker in conditions like chronic liver disease where it is linked to macrophage activation and fibrosis. Within these disorders proteins like LDL and inflammatory cytokines are closely associated with CD163 function and regulation.
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Product protocols

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Target data

Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
See full target information CD163
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 42:101867 PubMed38308919

2024

Transforming growth factor-β1 and soluble co-inhibitory immune checkpoints as putative drivers of immune suppression in patients with basal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mahlatse C M Kgokolo,Nonkululeko Z Malinga,Helen C Steel,Pieter W A Meyer,Teresa Smit,Ronald Anderson,Bernardo L Rapoport

Antioxidants (Basel, Switzerland) 12: PubMed37107322

2023

Regulation of CD163 Receptor in Patients with Abdominal Aortic Aneurysm and Associations with Antioxidant Enzymes HO-1 and NQO1.

Applications

Unspecified application

Species

Unspecified reactive species

Bianca Hamann,Anna Klimova,Felicia Klotz,Frieda Frank,Christian Jänichen,Marvin Kapalla,Pamela Sabarstinski,Steffen Wolk,Henning Morawietz,David M Poitz,Anja Hofmann,Christian Reeps

Experimental and therapeutic medicine 25:169 PubMed37006880

2023

IGFBP‑rP1 affects the proliferation, apoptosis and macrophage polarization of endometrial cancer cells by regulating the PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Gao,Shiqi Suo,Jingxia Li,Chunxia Wang,Ranran Deng,Yuxia Hu,Chunzheng Zhang

The Journal of allergy and clinical immunology 149:1592-1606.e16 PubMed35304157

2022

Distinguishing immune activation and inflammatory signatures of multisystem inflammatory syndrome in children (MIS-C) versus hemophagocytic lymphohistiocytosis (HLH).

Applications

Unspecified application

Species

Unspecified reactive species

Deepak Kumar,Christina A Rostad,Preeti Jaggi,D Sofia Villacis Nunez,Chengyu Prince,Austin Lu,Laila Hussaini,Thinh H Nguyen,Sakshi Malik,Lori A Ponder,Sreekala P V Shenoy,Evan J Anderson,Michael Briones,Ignacio Sanz,Sampath Prahalad,Shanmuganathan Chandrakasan
View all publications
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