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AB325896

Human CD163 ELISA Kit, Chemiluminescent

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Human CD163 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human CD163 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 43.9 - 32000 pg/ml with a sensitivity of 16 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

CD163, M130, Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor

6 Images
Sandwich ELISA - Human CD163 ELISA Kit, Chemiluminescent (AB325896)
  • sELISA

Lab

Sandwich ELISA - Human CD163 ELISA Kit, Chemiluminescent (AB325896)

Interpolated concentrations of native CD163 in stimulated human PBMC cell culture supernatant samples. PBMC samples were stimulated for 46 hours with 1.5% PHA-M. The concentrations of CD163 were measured in duplicates, interpolated from the CD163 standard curves and corrected for sample dilution. Undiluted samples are as follows : stimulated PBMC supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD163 concentration was determined to be 22 ng/mL in stimulated PBMC supernatant.

Sandwich ELISA - Human CD163 ELISA Kit, Chemiluminescent (AB325896)
  • sELISA

Lab

Sandwich ELISA - Human CD163 ELISA Kit, Chemiluminescent (AB325896)

Dynamic range comparison between the Chemiluminescent Human CD163 SimpleStep ELISA, colorimetric Human CD163 SimpleStep ELISA (ab274394), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and dynamic range.

Sandwich ELISA - Human CD163 ELISA Kit, Chemiluminescent (AB325896)
  • sELISA

Lab

Sandwich ELISA - Human CD163 ELISA Kit, Chemiluminescent (AB325896)

Standard curve comparison between the Chemiluminescent Human CD163 SimpleStep ELISA and colorimetric Human CD163 SimpleStep ELISA (ab274394) in Sample Diluent 25BS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CD163 ELISA Kit, Chemiluminescent (AB325896)
  • sELISA

Lab

Sandwich ELISA - Human CD163 ELISA Kit, Chemiluminescent (AB325896)

Interpolated concentrations of native CD163 in human serum and plasma samples. The concentrations of CD163 were measured in duplicates, interpolated from the CD163 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1%, plasma (citrate) 1%, plasma (heparin) 1%, and plasma (EDTA) 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD163 concentration was determined to be 704 ng/mL in serum, 552 ng/mL in plasma (citrate), 545 ng/ml in plasma (heparin), and 515 ng/mL in plasma (EDTA).

Sandwich ELISA - Human CD163 ELISA Kit, Chemiluminescent (AB325896)
  • sELISA

Lab

Sandwich ELISA - Human CD163 ELISA Kit, Chemiluminescent (AB325896)

Serum from ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD163 concentration was determined to be 563 ng/mL with a range of 338 – 1,030 ng/mL.

Sandwich ELISA - Human CD163 ELISA Kit, Chemiluminescent (AB325896)
  • sELISA

Lab

Sandwich ELISA - Human CD163 ELISA Kit, Chemiluminescent (AB325896)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 16 pg/mL

Range

43.9 - 32000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CD163 ELISA Kit, Chemiluminescent (ab325896) is a rapid single-wash sandwich 55-min ELISA to measure Human CD163 in Serum, Plasma – Citrate, Plasma – EDTA, Plasma – Heparin, Cell Culture Supernatant with a sensitivity of 16 pg/mL.

How the assay works

Human CD163 ELISA Kit, Chemiluminescent (ab325896) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human CD163 (ab325896) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-CD163 antibody [EPR22675-13] - BSA and Aside free (Capture) ab274440
- Anti-CD163 antibody [EPR22675-102] - BSA and Aside free (Detector) ab274441
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.6" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.5" } ]
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Recovery

[ { "sample": "Serum", "range": "103 - 117 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "90 - 102 %", "average": "= 97" }, { "sample": "Heparin Plasma", "range": "106 - 115 %", "average": "= 111" }, { "sample": "EDTA Plasma", "range": "105 - 120 %", "average": "= 111" }, { "sample": "Cell culture supernatant", "range": "100 - 112 %", "average": "= 106" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
See full target information CD163
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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