Human CD164 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human CD164 in Human in Cell culture supernatant, Plasma, Serum samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.04 - 10 ng/mL
- Sensitivity
- = 0.04 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Sialomucin that may play a key role in hematopoiesis by facilitating the adhesion of CD34(+) cells to the stroma and by negatively regulating CD34(+)CD38(lo/-) cell proliferation. Modulates the migration of umbilical cord blood CD133+ cells and this is mediated through the CXCL12/CXCR4 axis. May play an important role in prostate cancer metastasis and the infiltration of bone marrow by cancer cells. Promotes myogenesis by enhancing CXCR4-dependent cell motility. Positively regulates myoblast migration and promotes myoblast fusion into myotubes (By similarity).
Alternative names
CD164, Sialomucin core protein 24, MUC-24, Endolyn, Multi-glycosylated core protein 24, MGC-24, MGC-24v
What's included?
Recommended products
Human CD164 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human CD164 in Human in Cell culture supernatant, Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.04 - 10 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.04 ng/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 97.6 | Range 74 - 125 % |
Sample type Plasma | Average % = 103.4 | Range 75 - 137 % |
Sample type Cell culture media | Average % = 106.4 | Range 79 - 132 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human CD164 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD164 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human CD164 coated on a 96-well plate.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD164 also known as endolyn is a sialomucin protein with a molecular weight of approximately 80 to 90 kDa. It is a cell surface glycoprotein found on hematopoietic progenitor cells stromal cells epithelial cells and certain cancer cells. The expression of CD164 plays a part in the regulation of adhesion migration and cellular localization which affects cell proliferation and tissue development. It acts as a high-affinity receptor for novel inflammatory cytokines and modulates interactions with other cellular molecules.
- Biological function summary
CD164 influences the adhesive properties within specific cellular environments. It contributes to the formation of complexes with other cellular proteins to modulate cell communication and migration. The interaction of CD164 with integrins and cell surface receptors regulates cellular trafficking. It influences the behavior of cells at high confluency which impacts how these cells aggregate and differentiate in tissue structures.
- Pathways
More than four biochemical interactions influence CD164's role in controlling cellular movement and tissue architecture. It plays a significant role in the chemokine signaling pathway affecting memory B-cell homing. Furthermore CD164 is linked with the transforming growth factor-beta (TGF-beta) pathway through its association with integrins which regulates numerous cellular processes such as growth and development. By modulating these pathways it participates in the body's response to injury and inflammation.
- Associated diseases and disorders
Aberrant CD164 expression or function has been linked to various pathological conditions. For instance changes in CD164 levels are observed in certain leukemia types where its overexpression may contribute to abnormal cell growth and survival. Additionally its interaction with proteins like CXCR4 has implications in the metastasis of breast cancer enhancing the invasiveness of cancer cells. Therefore CD164's involvement in these diseases highlights its potential as a therapeutic target in cancer research.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Sandwich ELISA - Human CD164 ELISA Kit (ab313524)
Example of standard curve using ab313524. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com