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AB313524

Human CD164 ELISA Kit

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Human CD164 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CD164 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

CD164, Sialomucin core protein 24, MUC-24, Endolyn, Multi-glycosylated core protein 24, MGC-24, MGC-24v

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Sandwich ELISA - Human CD164 ELISA Kit (AB313524)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD164 ELISA Kit (AB313524)

Example of standard curve using ab313524. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.04 ng/mL

Range

0.04 - 10 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CD164 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD164 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human CD164 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "74 - 125 %", "average": "= 97.6" }, { "sample": "Plasma", "range": "75 - 137 %", "average": "= 103.4" }, { "sample": "Cell culture media", "range": "79 - 132 %", "average": "= 106.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313524-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin", "number":"AB313524-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313524-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313524-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human CD164 Detection Antibody", "number":"AB313524-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human CD164 Antibody-coated ELISA Plate", "number":"AB313524-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human CD164 Protein Standard", "number":"AB313524-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313524-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313524-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD164 also known as endolyn is a sialomucin protein with a molecular weight of approximately 80 to 90 kDa. It is a cell surface glycoprotein found on hematopoietic progenitor cells stromal cells epithelial cells and certain cancer cells. The expression of CD164 plays a part in the regulation of adhesion migration and cellular localization which affects cell proliferation and tissue development. It acts as a high-affinity receptor for novel inflammatory cytokines and modulates interactions with other cellular molecules.
Biological function summary

CD164 influences the adhesive properties within specific cellular environments. It contributes to the formation of complexes with other cellular proteins to modulate cell communication and migration. The interaction of CD164 with integrins and cell surface receptors regulates cellular trafficking. It influences the behavior of cells at high confluency which impacts how these cells aggregate and differentiate in tissue structures.

Pathways

More than four biochemical interactions influence CD164's role in controlling cellular movement and tissue architecture. It plays a significant role in the chemokine signaling pathway affecting memory B-cell homing. Furthermore CD164 is linked with the transforming growth factor-beta (TGF-beta) pathway through its association with integrins which regulates numerous cellular processes such as growth and development. By modulating these pathways it participates in the body's response to injury and inflammation.

Aberrant CD164 expression or function has been linked to various pathological conditions. For instance changes in CD164 levels are observed in certain leukemia types where its overexpression may contribute to abnormal cell growth and survival. Additionally its interaction with proteins like CXCR4 has implications in the metastasis of breast cancer enhancing the invasiveness of cancer cells. Therefore CD164's involvement in these diseases highlights its potential as a therapeutic target in cancer research.
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Product protocols

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Target data

Sialomucin that may play a key role in hematopoiesis by facilitating the adhesion of CD34(+) cells to the stroma and by negatively regulating CD34(+)CD38(lo/-) cell proliferation. Modulates the migration of umbilical cord blood CD133+ cells and this is mediated through the CXCL12/CXCR4 axis. May play an important role in prostate cancer metastasis and the infiltration of bone marrow by cancer cells. Promotes myogenesis by enhancing CXCR4-dependent cell motility. Positively regulates myoblast migration and promotes myoblast fusion into myotubes (By similarity).
See full target information CD164
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB240782

Anti-CD164 antibody [67D2]

Flow Cytometry - Anti-CD164 antibody [67D2] (AB240782)

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Proteins & Peptides

AB134891

Recombinant Human CD164 protein

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