JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB260063

Human CD167a/DDR1 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human CD167a/DDR1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD167a/DDR1 with a sensitivity of 143.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD167a, CAK, EDDR1, NEP, NTRK4, PTK3A, RTK6, TRKE, DDR1, Epithelial discoidin domain-containing receptor 1, Epithelial discoidin domain receptor 1, CD167 antigen-like family member A, Cell adhesion kinase, Discoidin receptor tyrosine kinase, HGK2, Mammary carcinoma kinase 10, Protein-tyrosine kinase 3A, Protein-tyrosine kinase RTK-6, TRK E, Tyrosine kinase DDR, Tyrosine-protein kinase CAK, MCK-10

3 Images
ELISA - Human CD167a/DDR1 ELISA Kit (AB260063)
  • ELISA

Supplier Data

ELISA - Human CD167a/DDR1 ELISA Kit (AB260063)
Sandwich ELISA - Human CD167a/DDR1 ELISA Kit (AB260063)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD167a/DDR1 ELISA Kit (AB260063)

The DDR1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CD167a/DDR1 ELISA Kit (AB260063)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD167a/DDR1 ELISA Kit (AB260063)

The concentrations of DDR1 were measured in duplicates, interpolated from the DDR1 standard curves and corrected for sample dilution. Undiluted samples are as follows : MCF7 supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DDR1 concentration was determined to 3,910.99 pg/mL in MCF7 supernatant.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 143.7 pg/mL

Range

312.5 - 20000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Assay Kits

AB267687

Human CD167a/DDR1 Antibody Pair - BSA and Azide free

RabMAb|Recombinant

Sandwich ELISA - Human CD167a/DDR1 Antibody Pair - BSA and Azide free (AB267687)

  • 0
  • 0
Primary Antibodies

AB255810

Anti-CD167a/DDR1 antibody [EPR22316-508]

RabMAb|Recombinant

Flow Cytometry - Anti-CD167a/DDR1 antibody [EPR22316-508] (AB255810)

  • 0
  • 0
Assay Kits

AB207621

Human Heme Oxygenase 1 ELISA Kit

Recombinant|SimpleStep

ELISA - Human Heme Oxygenase 1 ELISA Kit (AB207621)

  • 0
  • 0
Primary Antibodies

AB955

Anti-CD68 antibody [KP1]

Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (AB955)

  • 4
  • 48
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human CD167a/DDR1 ELISA Kit ab260063 is a rapid single-wash 90-min Sandwich ELISA to measure Human CD167a/DDR1 in cell culture supernatant. This SimpleStep sensitivity is 143.7 pg/mL.

How the assay works

Human CD167a/DDR1 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human CD167a/DDR1 ELISA Kit ab260063 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-CD167a/DDR1 antibody [EPR22316-121] - BSA and Azide free (Capture) ab259544
- Anti-CD167a/DDR1 antibody [EPR22316-2] - BSA and Azide free (Detector) ab259544

Epithelial discoidin domain-containing receptor 1 (DDR1) is a tyrosine kinase receptor involved in cell attachment to the extracellular matrix. DDR1 is also involved in cell proliferation and differentiation. DDR1's contribution to remodeling of the extracellular matrix supports cell migration and wound healing.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.68" } ]
style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": null, "average": "= 89" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab260063-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB260063-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB260063-CMP12", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB260063-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human DDR1 Lyophilized Recombinant Protein", "number":"AB260063-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB260063-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human DDR1 Capture Antibody", "number":"AB260063-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB260063-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB260063-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human DDR1 Detector Antibody", "number":"AB260063-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB260063-CMP06", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD167a also known as DDR1 (Discoidin Domain Receptor 1) is a receptor tyrosine kinase with a molecular mass of approximately 101 kDa. This protein is expressed widely in various tissues including the thyroid lung kidney and mammary gland. It becomes activated upon collagen binding leading to the regulation of cell adhesion proliferation and extracellular matrix remodeling. The DDR1 protein plays a mechanistic role in responding to unique collagen-rich environments within the body.
Biological function summary

CD167a/DDR1 modulates interactions between cells and their surrounding matrix. It is involved in a complex with collagen mediating signal transduction processes. This interaction is important for cellular biophysical responses driving processes like cell migration and differentiation. In marmoset thyroid tissue DDR1 helps maintain structural integrity and regulates cellular activities important for normal thyroid function.

Pathways

CD167a/DDR1 participates in key signaling pathways including those related to cell adhesion and extracellular matrix remodeling. Specifically it operates within the MAPK/ERK pathway influencing cell cycle dynamics and responses to stress signals. DDR1 also interacts with proteins like SHC and Ras enabling communication between the cell surface and intracellular targets necessary for growth and adaptation.

Mutations or irregular expression of CD167a/DDR1 have implications in cancer and fibrotic diseases. Overexpression or aberration of DDR1 can contribute to tumorigenesis facilitating tumor cell invasion and metastasis. In fibrotic conditions altered DDR1 activity can disrupt normal matrix remodeling linking it to proteins such as collagen I and III which play major roles in these disorders. Understanding DDR1's role offers potential for therapeutic interventions in targeting related pathological conditions.
style
left-column

Product protocols

style
left-column

Target data

Tyrosine kinase that functions as a cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing (By similarity). Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11.
See full target information DDR1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com