AB272195

Human CD177 ELISA Kit (PRV1)

Recombinant
SimpleStep
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Human CD177 ELISA Kit (PRV1) is a single-wash 90-min Simplestep used to quantify Human CD177 (PRV1) with a sensitivity of 12.83 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD177, NB1, PRV1, UNQ595/PRO1181, CD177 antigen, Human neutrophil alloantigen 2a, NB1 glycoprotein, Polycythemia rubra vera protein 1, HNA-2a, NB1 GP, PRV-1

2 Images

Sandwich ELISA - Human CD177 ELISA Kit (PRV1) (AB272195)

sELISA

Supplier Data

Sandwich ELISA - Human CD177 ELISA Kit (PRV1) (AB272195)

Example of human CD177 standard curve in Sample Diluent NS.

The CD177 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human CD177 ELISA Kit (PRV1) (AB272195)

Interpolated concentrations of native CD177 in human serum and plasma samples.

The concentrations of CD177 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 11,306 pg/mL in serum, 9,280 pg/mL in plasma (citrate), 9,383 pg/mL in plasma (EDTA), and 12,750 pg/mL in plasma (heparin).

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 12.83 pg/mL

Range

156.3 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human CD177 ELISA kit (ab272195) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD177 protein in serum and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD177 with 12.83 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

CD177 (PRV1) is a glycosyl-phosphatidylinositol (GPI)-linked cell surface glycoprotein that plays a role in neutrophil activation by physical association with β2 integrins, particularly CD11b/CD18, and in addition to forming complexes with neutrophil proteinase 3 for degranulation and superoxide production. CD177 can bind platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mouse, rat, cow share 57%, 49%, and 52% sequence homology with human CD177, respectively.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.3" } ]

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Recovery

[ { "sample": "Serum", "range": "86 - 97 %", "average": "= 91" }, { "sample": "EDTA Plasma", "range": "83 - 97 %", "average": "= 91" }, { "sample": "Heparin Plasma", "range": "83 - 93 %", "average": "= 88" }, { "sample": "Citrate plasma", "range": "95 - 102 %", "average": "= 98" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD177 also known as neutrophil antigen NB1 is a glycoprotein with an approximate mass of 58 kDa. It is primarily expressed on the surface of neutrophils a type of white blood cell involved in immune response. The expression of CD177 can vary among individuals where some people might have a higher or lower presence on their neutrophils. CD177 has been identified to play a role in cell adhesion and signaling functions contributing to various cellular processes within the immune system.

Biological function summary

CD177 functions by mediating interactions between neutrophils and endothelial cells during inflammation. This protein is not known to be part of a multi-protein complex but it plays a significant role in facilitating neutrophil transmigration from blood vessels to sites of tissue damage or infection. This process supports the body's defense mechanisms by enabling neutrophils to quickly respond to external threats and participate in the inflammatory response.

Pathways

The involvement of CD177 in chemotactic activity and cell migration highlights its contribution to both the immune response and inflammation pathways. Its interaction with proteins such as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1) enhances neutrophil transmigration across the endothelium. CD177 acts within these pathways to ensure a precise and rapid movement of neutrophils which is essential for an effective immune response.

CD177 levels have associations with conditions like polycythemia vera and anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis. In polycythemia vera CD177 can affect the proliferation of blood cells due to its role in neutrophil function. In ANCA-associated vasculitis CD177's interaction with proteins such as PR3 (Proteinase 3) on the surface of neutrophils could contribute to the pathophysiology of the disease leading to inappropriate activation of the immune system and subsequent tissue damage.

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Product protocols

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Target data

In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production (PubMed : 21193407). In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration (PubMed : 28807980). Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro (PubMed : 17580308). However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo (PubMed : 23461681). Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils (PubMed : 17244676, PubMed : 18462208). By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis (PubMed : 23202369).

See full target information CD177

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Product promise

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Human CD177 ELISA Kit (PRV1)

Sandwich ELISA - Human CD177 ELISA Kit (PRV1) (AB272195)

Sandwich ELISA - Human CD177 ELISA Kit (PRV1) (AB272195)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise