Human CD20 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human CD20 in Human in Plasma, Tissue Homogenate, Serum, Other biological fluids samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Tissue Homogenate, Serum, Other biological fluids
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.156 - 10 ng/mL
- Sensitivity
- < 0.094 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:12920111, PubMed:3925015, PubMed:7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:12920111, PubMed:18474602, PubMed:7684739).
Alternative names
CD20, MS4A1, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1
What's included?
Recommended products
Human CD20 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human CD20 in Human in Plasma, Tissue Homogenate, Serum, Other biological fluids samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Tissue Homogenate, Serum, Other biological fluids
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.156 - 10 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- < 0.094 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 97 | Range 89 - 105 % |
Sample type EDTA Plasma | Average % = 93 | Range 88 - 104 % |
Sample type Heparin Plasma | Average % = 96 | Range 87 - 103 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human CD20 ELISA Kit (ab285273, E4548) is a sandwich ELISA assay for the quantitative measurement of CD20 in human serum, plasma and cell culture supernatants. The density of color is proportional to the amount of CD20 captured from the samples.
No significant cross-reactivity or interference between antigen and analogues was observed.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.
- Biological function summary
CD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.
- Pathways
CD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.
- Associated diseases and disorders
CD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.
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1 product image
Sandwich ELISA - Human CD20 ELISA Kit (ab285273)
CD20 / MS4A1 (Human) ELISA Kit Standard Curve.
Typical Standard Curve: These standard curves are for demonstration only. A standard curve must be run with each assay.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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