JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB155429

Human CD200 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human CD200 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CD200 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD200, MOX1, MOX2, My033, OX-2 membrane glycoprotein

1 Images
Sandwich ELISA - Human CD200 ELISA Kit (AB155429)
  • sELISA

Unknown

Sandwich ELISA - Human CD200 ELISA Kit (AB155429)

CD200 Human ELISA Kit.

Representative standard curve using ab155429

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture media, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 20 pg/mL

Range

0.025 - 6 ng/mL

Assay Platform

Microplate

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's CD200 Human ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human CD200 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD200 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD200 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD200 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimisation may be required with urine samples.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "77 - 95 %", "average": "= 86.14" }, { "sample": "Plasma", "range": "67 - 86 %", "average": "= 78.35" }, { "sample": "Cell culture media", "range": "74 - 89 %", "average": "= 81.85" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab155429-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB155429-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB155429-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"240X HRP-Streptavidin Concentrate", "number":"AB155429-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB155429-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human CD200", "number":"AB155429-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB155429-CMP03", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB155429-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CD200 Microplate (12 x 8 wells)", "number":"AB155429-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human CD200 Standard (lyophilized)", "number":"AB155429-CMP07", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD200 also known as OX2 is a cell surface glycoprotein with a molecular mass of approximately 43 kDa. It plays a mechanical role in immune response regulation. CD200 expression occurs on several cell types such as thymocytes neurons vascular endothelium and splenocytes including cynomolgus monkey splenocytes. This protein generally modulates interactions between cells sending inhibitory signals through its receptor CD200R found on myeloid cells and certain lymphoid cells.
Biological function summary

CD200 regulates the immune system by inhibiting inflammatory responses and maintaining immune homeostasis. It does not act as part of a significant multi-protein complex but directly interacts with CD200R. This interaction promotes an anti-inflammatory state and is important in preventing autoimmune diseases and excessive tissue damage. The expression of CD200 is also an indicator of immune tolerance in various tissues.

Pathways

CD200 is involved in immune regulatory pathways. It plays a pivotal role in the suppression of immune responses through the CD200-CD200R signaling pathway. This pathway is vital for dampening inflammatory processes related to autoimmune reactions and is consequently linked to the maintenance of tissue homeostasis. Proteins related to CD200 through these pathways include CD200R and other molecules involved in immune checkpoint pathways.

CD200 expression correlates with several conditions including cancer and autoimmune diseases. The overexpression of CD200 in certain tumors promotes immune evasion thereby favoring tumor progression. Conversely reduced CD200 levels are associated with autoimmune disorders where a hyperactive immune system attacks self-tissues. Through these disease contexts CD200 operates alongside proteins such as CD200R and OX90 impacting disease progression and immune system modulation.
style
left-column

Product protocols

style
left-column

Target data

Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.
See full target information CD200
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB283877

Human CD46 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD46 ELISA Kit (AB283877)

  • 0
  • 0
Assay Kits

AB242239

Human CD86 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD86 ELISA Kit (AB242239)

  • 0
  • 0
Assay Kits

AB267801

Human CD38 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD38 ELISA Kit (AB267801)

  • 0
  • 0
Assay Kits

AB263875

Human BCMA ELISA Kit

Recombinant|SimpleStep

ELISA - Human BCMA ELISA Kit (AB263875)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com