Human CD200 ELISA Kit
- Recombinant
- SimpleStep
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Human CD200 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD200 with a sensitivity of 13 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
CD200, MOX1, MOX2, My033, OX-2 membrane glycoprotein
- sELISA
Supplier Data
Sandwich ELISA - Human CD200 ELISA Kit (AB267806)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). 9 out of 10 donors had detectable levels of CD200. The mean CD200 concentration was determined to be 369.8 pg/mL with a range of 212.9 – 566.1 pg/mL in the detectable donors.
- sELISA
Supplier Data
Sandwich ELISA - Human CD200 ELISA Kit (AB267806)
Interpolated concentrations of native CD200 in human serum and plasma samples.
The concentrations of CD200 were measured in duplicates, interpolated from the CD200 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 100%, plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD200 concentration was determined to be 384.9 pg/mL in serum, 351.3 pg/mL in plasma (citrate) and 398.2 pg/mL in plasma (EDTA), and 339.4 pg/mL in plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Human CD200 ELISA Kit (AB267806)
Example of human CD200 standard curve in Sample Diluent NS.
The CD200 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Reactivity data
Product details
Human CD200 ELISA Kit (ab267806) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD200 protein in cell culture media, edta plasma, hep plasma, serum, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD200 with 13 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Cluster of Differentiation 200 (CD200), also known as OX-2 membrane glycoprotein, is a 32 kDa protein that contains six glycosylation sites. CD200 belongs to the immunoglobulin superfamily of cell surface proteins and is found present at the first line of immune defense in which is involved in immunoregulatory functions such as to costimulates T-cell proliferation. ab267806 recognizes the extracellular domain of the full length transmembrane protein.
Precision
Recovery
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Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CD200 regulates the immune system by inhibiting inflammatory responses and maintaining immune homeostasis. It does not act as part of a significant multi-protein complex but directly interacts with CD200R. This interaction promotes an anti-inflammatory state and is important in preventing autoimmune diseases and excessive tissue damage. The expression of CD200 is also an indicator of immune tolerance in various tissues.
Pathways
CD200 is involved in immune regulatory pathways. It plays a pivotal role in the suppression of immune responses through the CD200-CD200R signaling pathway. This pathway is vital for dampening inflammatory processes related to autoimmune reactions and is consequently linked to the maintenance of tissue homeostasis. Proteins related to CD200 through these pathways include CD200R and other molecules involved in immune checkpoint pathways.
Product protocols
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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