AB272196

Human CD200R ELISA Kit (CD200R1)

Recombinant
SimpleStep
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Human CD200R ELISA Kit (CD200R1) is a single-wash 90-min Simplestep used to quantify Human CD200R (CD200R1) with a sensitivity of 11.89 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD200R, CRTR2, MOX2R, OX2R, UNQ2522/PRO6015, CD200R1, Cell surface glycoprotein CD200 receptor 1, CD200 cell surface glycoprotein receptor, Cell surface glycoprotein OX2 receptor 1

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Sandwich ELISA - Human CD200R ELISA Kit (CD200R1) (AB272196)

sELISA

Supplier Data

Sandwich ELISA - Human CD200R ELISA Kit (CD200R1) (AB272196)

Interpolated concentrations of native CD200R in human serum, and plasma samples.

The concentrations of CD200R were measured in duplicates, interpolated from the CD200R standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD200R concentration was determined to be 2,595 pg/mL in serum, 2,460 pg/mL in plasma (citrate), 2,904 pg/mL in plasma (EDTA), and 3,137 pg/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human CD200R ELISA Kit (CD200R1) (AB272196)

Interpolated concentrations of native CD200R in human HEL cell extract based on a 500 μg/mL extract load.

The concentrations of CD200R were measured in duplicate and interpolated from the CD200R standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD200R concentration was determined to be 817.1 pg/mL in HEL cell extract sample.

sELISA

Supplier Data

Sandwich ELISA - Human CD200R ELISA Kit (CD200R1) (AB272196)

Example of human CD200R standard curve in Sample Diluent NS.

The CD200R standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, Cell Lysate, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 11.89 pg/mL

Range

46.88 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human CD200R ELISA kit (ab272196) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD200R protein in serum, plasma and cell and tissue culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD200R with 11.89 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

CD200R (CD200R1), is a transmembrane glycoprotein member of the immunoglobin superfamily. CD200R acts as an inhibitory receptor for the CD200/OX-2 cell surface glycoprotein. CD200R is mainly expressed in myeloid cells and CD4+ T-cells and known to interact with CD200 in variety of cells including neurons, epithelial cells, endothelial cells, fibroblasts, and lymphoid cells. Activation of CD200R regulates the expression of proinflammatory molecules including TNF-alpha, interferons, and inducible nitric oxide synthase. Recent studies suggest that CD200R may prove useful in monitoring changes in bone metabolism. Mouse, rat and cow share 57%, 57% and 60% sequence homology with human CD200R.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.5" } ]

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Recovery

[ { "sample": "Serum", "range": "112 - 120 %", "average": "= 115" }, { "sample": "EDTA Plasma", "range": "81 - 110 %", "average": "= 93" }, { "sample": "Heparin Plasma", "range": "81 - 99 %", "average": "= 91" }, { "sample": "Cell Lysate", "range": "90 - 116 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "83 - 109 %", "average": "= 92" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab272196-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB272196-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB272196-CMP11", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB272196-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB272196-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB272196-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB272196-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB272196-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD200R (CD200R1) Capture Antibody", "number":"AB272196-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CD200R (CD200R1) Lyophilized Recombinant Protein", "number":"AB272196-CMP12", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB272196-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD200R (CD200R1) Detector Antibody", "number":"AB272196-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB272196-CMP05", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD200R also known as CD200 receptor is a protein expressed mainly on myeloid cells including macrophages dendritic cells and certain T cells. The protein has a molecular mass of approximately 62 kDa. This receptor is a type I membrane glycoprotein that plays an important role in immune system regulation. It binds to its ligand CD200 protein found on various cell types such as neurons epithelial cells and some immune cells to mediate suppressive effects on the immune response.

Biological function summary

CD200R plays a significant role in controlling the immune response by acting as an inhibitory receptor. When activated it sends signals that dampen immune cell activity which can prevent overactive immune responses and promote tissue homeostasis. This receptor is not part of a large receptor complex but interacts directly with its ligand CD200 to initiate signaling cascades that modulate immune functions. Its primary role lies in preventing unnecessary inflammation and autoimmunity through negative regulation of macrophage activation.

Pathways

CD200R is involved in the regulatory pathways of the immune system. It participates in pathways that manage immune tolerance and its interactions impact pathways related to inflammation control and tissue repair. The CD200R signaling cascades intersect with pathways involving other immune-modulatory proteins such as CD200R1 and CD200R3 which contribute to cellular communication in the immune environment. These pathways play critical roles in downregulating inflammatory responses and maintaining immune balance.

CD200R holds relevance for conditions involving immune dysregulation. It is connected to autoimmune disorders and atopic dermatitis. For example in atopic dermatitis altered expression or function of CD200R may lead to improper immune responses contributing to inflammation. Furthermore CD200R agonists which are molecules that mimic the function of CD200 are an area of interest for developing therapeutic strategies to modulate immune responses. Related proteins such as CD200 and its other receptor variants play active roles in these disorders highlighting the importance of their balanced function to maintain immune homeostasis.

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Product protocols

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Target data

Inhibitory receptor for the CD200/OX2 cell surface glycoprotein. Limits inflammation by inhibiting the expression of pro-inflammatory molecules including TNF-alpha, interferons, and inducible nitric oxide synthase (iNOS) in response to selected stimuli. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200.

See full target information CD200R1

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websiteProtocolBooklet

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human CD200R ELISA Kit (CD200R1)

Sandwich ELISA - Human CD200R ELISA Kit (CD200R1) (AB272196)

Sandwich ELISA - Human CD200R ELISA Kit (CD200R1) (AB272196)

Sandwich ELISA - Human CD200R ELISA Kit (CD200R1) (AB272196)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Assay Kits

Assay Kits

Product promise