Human CD208 (LAMP3) ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human CD208 (LAMP3) with a sensitivity of 9.514 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 46.875 - 3000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 9.514 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Lysosomal membrane glycoprotein which plays a role in the unfolded protein response (UPR) that contributes to protein degradation and cell survival during proteasomal dysfunction (PubMed:25681212). Plays a role in the process of fusion of the lysosome with the autophagosome, thereby modulating the autophagic process (PubMed:24434718). Promotes hepatocellular lipogenesis through activation of the PI3K/Akt pathway (PubMed:29056532). May also play a role in dendritic cell function and in adaptive immunity (PubMed:9768752). (Microbial infection) Plays a positive role in post-entry steps of influenza A virus replication, either virus uncoating, cytosolic transport, or nuclear import of viral components, and promotes nuclear accumulation of influenza nucleoprotein/NP at early stages of viral infection. (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F. (Microbial infection) Promotes the intracellular proliferation of Salmonella typhimuium.
Alternative names
CD208, DCLAMP, TSC403, LAMP3, Lysosome-associated membrane glycoprotein 3, LAMP-3, Lysosomal-associated membrane protein 3, DC-lysosome-associated membrane glycoprotein, Protein TSC403, DC LAMP
What's included?
Recommended products
Human CD208 (LAMP3) ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human CD208 (LAMP3) with a sensitivity of 9.514 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 46.875 - 3000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 9.514 pg/mL
- General recovery
- 109%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 4 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 2.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 109 | Range 104 - 114 % |
Sample type Citrate plasma | Average % = 103 | Range 88 - 115 % |
Sample type Cell culture supernatant | Average % = 102 | Range 97 - 111 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human CD208 (LAMP3) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD208 (LAMP3) protein in Serum, Cit Plasma, Cell culture supernatant. Quantitate Human CD208 (LAMP3) with 9.514 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein CD208 also known as DC-LAMP or LAMP-3 is integral to the immune system. It has an approximate mass of 60 kDa. CD208 is a type I transmembrane glycoprotein found mainly in the endosomal/lysosomal compartments of dendritic cells. The expression of CD208 is upregulated during the maturation of dendritic cells making it a reliable marker for these immune cells. Researchers often use CD208 to study dendritic cell biology and its role in immune responses.
- Biological function summary
CD208 participates in the regulation of the immune response by facilitating antigen processing and presentation. This protein is a part of the lysosomal-associated membrane protein family which contributes to the recycling of MHC class II molecules. Through its involvement in the endocytic pathway CD208 aids in presenting antigens to T lymphocytes an important step in triggering adaptive immunity. The protein colocalizes with MHC class II which is essential for effective antigen presentation.
- Pathways
CD208 has a significant role in the endosomal-lysosomal pathway where it interacts with other proteins involved in antigen processing. It also engages in the intracellular trafficking pathway that affects the presentation of peptides on MHC class II molecules. The interaction with proteins such as HLA-DR is vital for efficient antigen presentation to helper T cells. CD208 supports the proper delivery of peptide-loaded MHC molecules to the cell surface a necessity for a functional adaptive immune response.
- Associated diseases and disorders
CD208 has implications in autoimmune diseases and infectious diseases. Its expression is notably altered in conditions like lupus and certain infectious diseases. In systemic lupus erythematosus (SLE) abnormal CD208 expression correlates with the dysregulation of immune responses. Additionally during infectious diseases changes in CD208 expression can impact the activation and function of dendritic cells affecting disease progression. The interaction of CD208 with proteins like MHC class II molecules highlights its role in disease pathology and immune regulation.
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5 product images
Sandwich ELISA - Human CD208 (LAMP3) ELISA Kit- Extracellular (ab320797)
Example of human CD208 standard curve in Sample Diluent NS. Example of human CD208 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human CD208 (LAMP3) ELISA Kit- Extracellular (ab320797)
Interpolated concentrations of human CD208 in human serum and plasma – citrate. Interpolated concentration of native CD208 was measured in duplicate at different sample concentrations. Undiluted samples are 50% human serum and 50% plasma – citrate. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human CD208 (LAMP3) ELISA Kit- Extracellular (ab320797)
Interpolated concentrations of CD208 in normal human serum donors. Serum of eight individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean CD208 concentration was determined to be 786.8 pg/mL with a range of 472.9 – 1,209.6 pg/mL.
Sandwich ELISA - Human CD208 (LAMP3) ELISA Kit- Extracellular (ab320797)
Example of human CD208 standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Example of human CD208 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human CD208 (LAMP3) ELISA Kit- Extracellular (ab320797)
Interpolated concentrations of human CD208 in 96-well vs. 384-well plates. Interpolated concentration of native CD208 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50% human serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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