JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB320798

Human CD208 (LAMP3) ELISA Kit- Intracellular

Be the first to review this product! Submit a review

|

(0 Publication)

Human CD208 (LAMP3) ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human CD208 (LAMP3) with a sensitivity of 9.234 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput

View Alternative Names

CD208, DCLAMP, TSC403, LAMP3, Lysosome-associated membrane glycoprotein 3, LAMP-3, Lysosomal-associated membrane protein 3, DC-lysosome-associated membrane glycoprotein, Protein TSC403, DC LAMP

4 Images
Sandwich ELISA - Human CD208 (LAMP3) ELISA Kit- Intracellular (AB320798)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD208 (LAMP3) ELISA Kit- Intracellular (AB320798)

Interpolated concentrations of human CD208 in 96-well vs. 384-well plates. Interpolated concentration of native CD208 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1.0 mg/mL A-431 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human CD208 (LAMP3) ELISA Kit- Intracellular (AB320798)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD208 (LAMP3) ELISA Kit- Intracellular (AB320798)

Interpolated concentrations of human CD208 in A-431 cell extract, HEK-293 cell extract, and human lung tissue extract. Interpolated concentration of native CD208 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : A-431 cell extract 1.0 mg/mL, HEK-293 cell extract 1.0 mg/mL, and human lung tissue extract 0.25 mg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human CD208 (LAMP3) ELISA Kit- Intracellular (AB320798)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD208 (LAMP3) ELISA Kit- Intracellular (AB320798)

Example of human CD208 standard curve in 1X Cell Extraction Buffer PTR. Example of human CD208 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CD208 (LAMP3) ELISA Kit- Intracellular (AB320798)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD208 (LAMP3) ELISA Kit- Intracellular (AB320798)

Example of human CD208 standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate. Example of human CD208 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 9.234 pg/mL

Range

46.875 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human CD208 (LAMP3) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD208 (LAMP3) protein in Cell Lysate, Tissue extracts. Quantitate Human CD208 (LAMP3) with 9.234 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.9" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.7" } ]
style
left-column

Recovery

[ { "sample": "Cell Lysate", "range": "103 - 106 %", "average": "= 104" }, { "sample": "Tissue Extracts", "range": "107 - 121 %", "average": "= 114" } ]
style
left-column

What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab320798-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB320798-CMP09" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB320798-CMP08" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB320798-CMP10" }, { "size":"1 x 200 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB320798-CMP05" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB320798-CMP04" }, { "size":"20 x 1 Vial", "name":"Human CD208 (LAMP3) Lyophilized Recombinant Protein", "number":"AB320798-CMP03" }, { "size":"10 x 1 Vial", "name":"Lyophilized Human CD208 (LAMP3) Capture Antibody", "number":"AB320798-CMP01" }, { "size":"10 x 600 µL", "name":"10X Human CD208 (LAMP3) Detector Antibody", "number":"AB320798-CMP02" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB320798-CMP11" }, { "size":"1 x 50 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB320798-CMP06" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab320798-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB320798-CMP09" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB320798-CMP08" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB320798-CMP11" }, { "size":"1 x 20 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB320798-CMP05" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB320798-CMP04" }, { "size":"2 x 1 Vial", "name":"Human CD208 (LAMP3) Lyophilized Recombinant Protein", "number":"AB320798-CMP03" }, { "size":"1 x 1 Vial", "name":"Lyophilized Human CD208 (LAMP3) Capture Antibody", "number":"AB320798-CMP01" }, { "size":"1 x 600 µL", "name":"10X Human CD208 (LAMP3) Detector Antibody", "number":"AB320798-CMP02" }, { "size":"1 x 50 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB320798-CMP06" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB320798-CMP12" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab320798-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB320798-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB320798-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB320798-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB320798-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB320798-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB320798-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CD208 (LAMP3) Lyophilized Recombinant Protein", "number":"AB320798-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lyophilized Human CD208 (LAMP3) Capture Antibody", "number":"AB320798-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD208 (LAMP3) Detector Antibody", "number":"AB320798-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB320798-CMP11", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB320798-CMP06", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein CD208 also known as DC-LAMP or LAMP-3 is integral to the immune system. It has an approximate mass of 60 kDa. CD208 is a type I transmembrane glycoprotein found mainly in the endosomal/lysosomal compartments of dendritic cells. The expression of CD208 is upregulated during the maturation of dendritic cells making it a reliable marker for these immune cells. Researchers often use CD208 to study dendritic cell biology and its role in immune responses.
Biological function summary

CD208 participates in the regulation of the immune response by facilitating antigen processing and presentation. This protein is a part of the lysosomal-associated membrane protein family which contributes to the recycling of MHC class II molecules. Through its involvement in the endocytic pathway CD208 aids in presenting antigens to T lymphocytes an important step in triggering adaptive immunity. The protein colocalizes with MHC class II which is essential for effective antigen presentation.

Pathways

CD208 has a significant role in the endosomal-lysosomal pathway where it interacts with other proteins involved in antigen processing. It also engages in the intracellular trafficking pathway that affects the presentation of peptides on MHC class II molecules. The interaction with proteins such as HLA-DR is vital for efficient antigen presentation to helper T cells. CD208 supports the proper delivery of peptide-loaded MHC molecules to the cell surface a necessity for a functional adaptive immune response.

CD208 has implications in autoimmune diseases and infectious diseases. Its expression is notably altered in conditions like lupus and certain infectious diseases. In systemic lupus erythematosus (SLE) abnormal CD208 expression correlates with the dysregulation of immune responses. Additionally during infectious diseases changes in CD208 expression can impact the activation and function of dendritic cells affecting disease progression. The interaction of CD208 with proteins like MHC class II molecules highlights its role in disease pathology and immune regulation.
style
left-column

Product protocols

style
left-column

Target data

Lysosomal membrane glycoprotein which plays a role in the unfolded protein response (UPR) that contributes to protein degradation and cell survival during proteasomal dysfunction (PubMed : 25681212). Plays a role in the process of fusion of the lysosome with the autophagosome, thereby modulating the autophagic process (PubMed : 24434718). Promotes hepatocellular lipogenesis through activation of the PI3K/Akt pathway (PubMed : 29056532). May also play a role in dendritic cell function and in adaptive immunity (PubMed : 9768752).. (Microbial infection) Plays a positive role in post-entry steps of influenza A virus replication, either virus uncoating, cytosolic transport, or nuclear import of viral components, and promotes nuclear accumulation of influenza nucleoprotein/NP at early stages of viral infection.. (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.. (Microbial infection) Promotes the intracellular proliferation of Salmonella typhimuium.
See full target information LAMP3
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Primary Antibodies

AB271053

Anti-CD208 antibody [EPR24265-8]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Multiplex immunohistochemistry - Anti-CD208 antibody [EPR24265-8] (AB271053)

  • 0
  • 0
Proteins & Peptides

AB276290

Recombinant Human CD208 protein (His tag)

SDS-PAGE - Recombinant Human CD208 protein (His tag) (AB276290)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com