JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB45909

Human CD21 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human CD21 ELISA Kit is a Sandwich ELISA for the measurement of Human CD21 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD21, C3DR, CR2, Complement receptor type 2, Cr2, Complement C3d receptor, Epstein-Barr virus receptor, EBV receptor

1 Images
Sandwich ELISA - Human CD21 ELISA Kit (AB45909)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD21 ELISA Kit (AB45909)

Representative Standard Curve using ab45909.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 3 U/mL

Range

3.12 - 100 U/mL

Assay time

1h 45m

Assay Platform

Microplate

style
left-column

Complementary Products

Assay Kits

AB267801

Human CD38 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD38 ELISA Kit (AB267801)

  • 0
  • 0
Primary Antibodies

AB227662

Anti-CD21 antibody [SP186] - C-terminal

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD21 antibody [SP186] - C-terminal (AB227662)

  • 0
  • 0
Assay Kits

AB190814

Human CD31 ELISA Kit (PECAM)

Recombinant|SimpleStep

Sandwich ELISA - Human CD31 ELISA Kit (PECAM) (AB190814)

  • 0
  • 0
Assay Kits

AB287168

Human CD44 ELISA Kit

Recombinant|SimpleStep

ELISA - Human CD44 ELISA Kit (AB287168)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's CD21 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD21 in Human serum, plasma, buffered solutions and cell culture media.

A monoclonal antibody specific for CD21 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD21 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD21 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD21 present in the samples.

This kit will recognize both endogenous and recombinant Human CD21.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 8, "mean": "51.13 U/mL", "standardDeviation": "2.29", "coefficientOfVariability": "= 4.47" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 8, "mean": "10.44 U/mL", "standardDeviation": "0.46", "coefficientOfVariability": "= 4.4" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 8, "mean": "10.76 U/mL", "standardDeviation": "0.56", "coefficientOfVariability": "= 5.2" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 8, "mean": "50.25 U/mL", "standardDeviation": "2.24", "coefficientOfVariability": "= 4.45" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab45909-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB45909-CMP03" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB45909-CMP02" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB45909-CMP05" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB45909-CMP10" }, { "size":"1 x 1 Unit", "name":"CD21 Microplate (12 x 8 well strips)", "number":"AB45909-CMP08" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB45909-CMP06" }, { "size":"1 x 400 µL", "name":"Biotinylated anti-CD21 antibody", "number":"AB45909-CMP07" }, { "size":"1 x 7.5 mL", "name":"Biotinylated Antibody Diluent", "number":"AB45909-CMP01" }, { "size":"2 x 1 Vial", "name":"CD21 Standard", "number":"AB45909-CMP09" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB45909-CMP04" } ] }, "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab45909-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB45909-CMP03", "productcode":"" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB45909-CMP10", "productcode":"" }, { "size":"4 x 1 Vial", "name":"CD21 Standard", "number":"AB45909-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB45909-CMP06", "productcode":"" }, { "size":"1 x 2 Unit", "name":"CD21 Microplate (12 x 8 well strips)", "number":"AB45909-CMP08", "productcode":"" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB45909-CMP01", "productcode":"" }, { "size":"2 x 400 µL", "name":"Biotinylated anti-CD21 antibody", "number":"AB45909-CMP07", "productcode":"" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB45909-CMP02", "productcode":"" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB45909-CMP04", "productcode":"" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB45909-CMP05", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD21 also known as complement receptor type 2 (CR2) or BU32 is a protein primarily expressed on the surface of B cells and some T cells. It functions as a receptor for complement component C3d EBV (Epstein-Barr Virus) and IFN-α. CD21 weighs approximately 145 kDa and belongs to the membrane protein family. Detection of CD21 can be done via immunohistochemistry (IHC) and blood tests. This marker is valuable for identifying cell populations that have roles in immune responses.
Biological function summary

Complement receptor type 2 is key in the immune system by facilitating B cell activation and proliferation. CD21 forms complexes with CD19 and CD81 increasing the sensitivity of B cells to antigens. This interaction enhances signal transduction enabling B lymphocytes to respond effectively to pathogens. The protein also plays a role in regulating immune tolerance and maintaining humoral immunity being important not only in immune defense but also in preventing autoimmunity.

Pathways

CD21 interacts with complement activation and immune response pathways. It connects with CD19 in a pivotal role where it further amplifies signaling in B lymphocytes. This interaction strengthens the downstream signaling of BCR (B cell receptor) important for antibody production. CD21 contributes to bridging the innate and adaptive immunological pathways by engaging components like C3d.

CD21 shows significant relevance in systemic lupus erythematosus (SLE) and some B cell lymphomas. Deficiencies or mutations in CD21 can lead to defective immune responses and increased susceptibility to autoimmune conditions such as SLE where CD19 is also implicated. Furthermore in Epstein-Barr Virus infections CD21 serves as an entry receptor facilitating pathogen establishment which can result in chronic conditions or even malignancies.
style
left-column

Product protocols

style
left-column

Target data

Serves as a receptor for various ligands including complement component CD3d, HNRNPU OR IFNA1 (PubMed : 1849076, PubMed : 21527715, PubMed : 7753047). When C3d is bound to antigens, attaches to C3d on B-cell surface and thereby facilitates the recognition and uptake of antigens by B-cells (PubMed : 21527715). This interaction enhances B-cell activation and subsequent immune responses. Forms a complex with several partners on the surface of B-cells including CD19, FCRL5 and CD81, to form the B-cell coreceptor complex that plays a crucial role in B-cell activation and signaling (PubMed : 1383329, PubMed : 30107486). Also induces specific intracellular signaling separately from the BCR and CD19 by activating the tyrosine kinase SRC, which then phosphorylates nucleolin/NCL and triggers AKT and GSK3 kinase activities in a SYK/CD19-independent manner (PubMed : 12938232). Acts as a ligand for CD23 (FcepsilonRII), a low-affinity receptor for IgE, which is expressed on B-cells and other immune cells, and thus participates in the regulation of IgE production (PubMed : 1386409).. (Microbial infection) Acts as a receptor for Epstein-Barr virus.
See full target information CR2
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com