Human CD226 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CD226 in Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Colorimetric
Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma
Sandwich (quantitative)
Human
12.5 - 800 pg/mL
1h 30m
= 1.58 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells (PubMed:8673704, PubMed:9712030). Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells (PubMed:26755705, PubMed:31253644, PubMed:30591568). Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes (By similarity).
DNAM1, CD226, CD226 antigen, DNAX accessory molecule 1, DNAM-1
Human CD226 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CD226 in Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Colorimetric
Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma
Sandwich (quantitative)
Human
12.5 - 800 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 1.58 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 2.9 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 3.2 |
Sample type | Average % | Range |
---|---|---|
Sample type Cell culture supernatant | Average % = 97 | Range 95 - 98 % |
Sample type Serum | Average % = 93 | Range 86 - 98 % |
Sample type EDTA Plasma | Average % = 96 | Range 92 - 102 % |
Sample type Heparin Plasma | Average % = 90 | Range 83 - 102 % |
Sample type Citrate plasma | Average % = 91 | Range 85 - 97 % |
Blue Ice
+4°C
+4°C
+4°C
Human CD226 ELISA kit (ab282303) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CD226 protein in human serum, plasma, and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD226 with 1.58 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Human CD226, a glycoprotein, is expressed on the surface of NK cells, platelets, monocytes, and a subset of T cells. CD226 is involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cells. CD226 is a cell surface receptor for NECTIN2 and upon ligand binding, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Human CD226 shares 53%, 51%, 61% and, 94% sequence homology with mouse, rat, cow, and monkey, respectively.
This supplementary information is collated from multiple sources and compiled automatically.
CD226 also known as DNAM-1 (DNAX Accessory Molecule-1) is a transmembrane protein expressed on the surface of various immune cells such as T cells natural killer (NK) cells and some B cells. The protein has a molecular weight of approximately 65 kDa. CD226 is part of the Ig superfamily and plays a role in the adhesion and signaling processes of immune cells. It interacts with its ligands CD112 (PVRL2) and CD155 (PVR) on target cells facilitating immune synapse formation and cytotoxic activity.
CD226 is involved in immune cell activation and receptor-mediated signaling. It aids the recognition and destruction of target cells by NK cells and cytotoxic T lymphocytes through its interaction with ligand-expressing cells. CD226 does not work alone but functions as part of a complex system of co-stimulatory and inhibitory receptors balancing immune responses. Its cooperation with receptors such as CD96 and TIGIT (T cell immunoreceptor with Ig and ITIM domains) modulates the activity of immune effector cells.
CD226 takes part in immune signaling pathways that regulate cell-mediated cytotoxicity and inflammation. It is an important component of the immune checkpoint axis interacting closely with proteins like CD96 and TIGIT within these pathways. This interaction helps determine the fate of immune responses influencing the progression or resolution of inflammatory and autoimmune conditions. CD226 plays a role in the NK cell-mediated cytotoxicity pathway and the adaptive immune response.
CD226 has been linked to autoimmune diseases such as multiple sclerosis and type 1 diabetes. Abnormal expression or function of CD226 can influence the pathogenesis of these conditions often through interactions with related proteins like TIGIT impacting immune tolerance and effector cell function. CD226's involvement in regulating immune responses highlights its importance as a potential therapeutic target in managing autoimmune and other immune-related disorders.
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Example of human CD226 standard curve in Sample Diluent NS.
The CD226 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentrations of native CD226 in human serum, plasma, and cell culture supernatant samples.
The concentrations of CD226 were measured in duplicates, interpolated from the CD226 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 5%, plasma (citrate) 10%, plasma (EDTA) 10%, plasma (heparin) 2.5%, and PBMC supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD226 concentration was determined to be 5.77 ng/mL in serum, 3.72 ng/mL in plasma (citrate), 2.73 ng/mL in plasma (EDTA), 22.06 ng/mL in plasma (heparin), and 0.34 ng/mL in PBMC supernatant.
2.5% Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD226 concentration was determined to be 9.36 ng/mL with a range of 2.62 – 25.51 ng/mL.
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