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AB277431

Human CD229 ELISA Kit

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Human CD229 ELISA Kit is a Sandwich ELISA for the measurement of Human CD229 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD229, CDABP0070, LY9, T-lymphocyte surface antigen Ly-9, Cell surface molecule Ly-9, Lymphocyte antigen 9, SLAM family member 3, Signaling lymphocytic activation molecule 3, SLAMF3

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ELISA - Human CD229 ELISA Kit (AB277431)
  • ELISA

Supplier Data

ELISA - Human CD229 ELISA Kit (AB277431)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 80 pg/mL

Range

81.92 - 20000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CD229 ELISA Kit (ab277431) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD229 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human CD229 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human CD229 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human CD229 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human CD229 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "90 - 142 %", "average": "= 117.1" }, { "sample": "Plasma", "range": "90 - 145 %", "average": "= 121" }, { "sample": "Cell culture media", "range": "95 - 144 %", "average": "= 119.8" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277431-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277431-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277431-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human CD229 coated Microplate", "number":"AB277431-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human CD229 Antibody", "number":"AB277431-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"700X HRP-Streptavidin Concentrate", "number":"AB277431-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277431-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB277431-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB277431-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CD229 standard protein (Lyophilized)", "number":"AB277431-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD229 also known as SLAMF3 or Ly9 is a transmembrane protein that weighs approximately 80 kDa. This protein belongs to the CD2 subset of the immunoglobulin superfamily. You can find high expression of CD229 in various lymphoid tissues such as the spleen thymus and peripheral blood lymphocytes. Its expression is notably high in T cells B cells and natural killer (NK) cells. As a homophilic receptor CD229 aids in cell-cell adhesion playing an essential role in immune response regulation among lymphocytes.
Biological function summary

CD229 influences signaling within immune cells. It functions as a mediator of adhesion and activation signaling pathways. CD229 does not act alone and often works as part of a receptor complex that may include CD48 and other members of the SLAM family. Besides its role in cellular adhesion CD229 participates in balancing immune activation and immune tolerance demonstrating its importance in developing and maintaining a robust immune response.

Pathways

CD229 participates in the adaptive immune signaling pathways. It links with other signaling proteins such as SAP (SLAM-associated protein) a critical modulator of downstream signaling cascades. SAP interacts with SLAM-family receptors including CD229 to regulate T and B cell activation and function. The involvement of CD229 in various immune signaling pathways positions it as a significant player in the broader context of cellular communication and response within the immune system.

CD229's aberrant expression or dysfunction links to autoimmune diseases such as systemic lupus erythematosus (SLE) and certain hematological malignancies. Altered CD229 expression has been observed in association with SLE where it may contribute to dysregulated immune responses. In hematological cancers CD229 overexpression or mutations can disrupt immune cell regulation promoting tumor progression. CD229 may interact with other SLAM-family proteins like CD244 which have implications in disease pathology and can be targets for therapeutic interventions.
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Product protocols

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Target data

Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. May participate in adhesion reactions between T lymphocytes and accessory cells by homophilic interaction. Promotes T-cell differentiation into a helper T-cell Th17 phenotype leading to increased IL-17 secretion; the costimulatory activity requires SH2D1A (PubMed : 22184727). Promotes recruitment of RORC to the IL-17 promoter (PubMed : 22989874). May be involved in the maintenance of peripheral cell tolerance by serving as a negative regulator of the immune response. May disable autoantibody responses and inhibit IFN-gamma secretion by CD4(+) T-cells. May negatively regulate the size of thymic innate CD8(+) T-cells and the development of invariant natural killer T (iNKT) cells (By similarity).
See full target information LY9
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websiteProtocolBooklet
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Product promise

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