Human CD23 ELISA Kit
- Recombinant
- SimpleStep
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- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- sELISA
Supplier Data
Sandwich ELISA - Human CD23 ELISA Kit (AB272468)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD23 concentration was determined to be 2,495 pg/mL with a range of 1,609 – 4,772 pg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human CD23 ELISA Kit (AB272468)
Interpolated concentrations of native CD23 in human urine, Raji cell culture supernatant and PBMC cell culture supernatant samples treated with or without 1.5% PHA for 36 hours.
The concentrations of CD23 were measured in duplicates, interpolated from the CD23 standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 25%, Raji supernatant 50%, PBMC PHA treated supernatant 7% and PBMC untreated supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD23 concentration was determined to be 4,289 pg/mL in neat urine, 4,358 pg/mL in neat Raji supernatant, 23,923 pg/mL in neat PBMC PHA treated supernatant and 498 pg/mL in PBMC untreated supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Human CD23 ELISA Kit (AB272468)
Interpolated concentrations of native CD23 in human serum and plasma samples.
The concentrations of CD23 were measured in duplicates, interpolated from the CD23 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 25%, plasma (EDTA) 100% and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD23 concentration was determined to be 1,741 pg/mL in neat serum, 1,601 pg/mL in neat plasma (citrate), 1,476 pg/mL in neat plasma (EDTA) and 1,930 pg/mL in neat plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Human CD23 ELISA Kit (AB272468)
Example of human CD23 standard curve in Sample Diluent NS.
The CD23 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Reactivity data
Product details
Human CD23 ELISA kit (ab272199) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CD23 protein in serum, plasma, urine and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD23 with 12 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
CD23, also known as Low Affinity Immunoglobulin Epsilon Fc Receptor, is a low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. It has essential roles in the regulation of IgE production and in the differentiation of B-cells. It is a B-cell-specific antigen. CD23 is a 47 kDa type II transmembrane glycoprotein containing a short N-terminal cytoplasmic tail, a transmembrane domain, and a large C-terminal extracellular domain containing C-type lectin domain. A soluble CD23 form also exists.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CD23 facilitates several immune functions including the regulation of immunoglobulin E (IgE) production and its receptor-mediated endocytosis. It has a role in cell adhesion growth and differentiation. CD23 exists independently localizing primarily to the surface of CD23-positive cells. Furthermore this protein influences the release and activity of various cytokines impacting both innate and adaptive immunity.
Pathways
CD23 plays central roles in IgE-mediated immune responses and allergic reactions. It is importantly involved in the synthesis and regulation of IgE connecting it to the IgE signaling pathway and immune system pathways. CD23 interacts with IgE and its receptors which include the high-affinity receptor FcεRI forming an interface between the antigen and immune effector functions. Additionally CD23 participates in pathways that involve immune response modulation and inflammation prevention.
Product protocols
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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