Human CD244 ELISA Kit (2B4) is a Sandwich (quantitative) ELISA kit for the measurement of Human CD244 (2B4) in Human in Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 15.6 - 1000 pg/mL
- Assay time
- 3h 30m
- Sensitivity
- < 10 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Heterophilic receptor of the signaling lymphocytic activation molecule (SLAM) family; its ligand is CD48. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Acts as activating natural killer (NK) cell receptor (PubMed:10359122, PubMed:11714776, PubMed:8376943). Activating function implicates association with SH2D1A and FYN (PubMed:15713798). Downstreaming signaling involves predominantly VAV1, and, to a lesser degree, INPP5D/SHIP1 and CBL. Signal attenuation in the absence of SH2D1A is proposed to be dependent on INPP5D and to a lesser extent PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10934222, PubMed:15713798). Stimulates NK cell cytotoxicity, production of IFN-gamma and granule exocytosis (PubMed:11714776, PubMed:8376943). Optimal expansion and activation of NK cells seems to be dependent on the engagement of CD244 with CD48 expressed on neighboring NK cells (By similarity). Acts as costimulator in NK activation by enhancing signals by other NK receptors such as NCR3 and NCR1 (PubMed:10741393). At early stages of NK cell differentiation may function as an inhibitory receptor possibly ensuring the self-tolerance of developing NK cells (PubMed:11917118). Involved in the regulation of CD8(+) T-cell proliferation; expression on activated T-cells and binding to CD48 provides costimulatory-like function for neighboring T-cells (By similarity). Inhibits inflammatory responses in dendritic cells (DCs) (By similarity).
Alternative names
CD244, 2B4, Natural killer cell receptor 2B4, NK cell activation-inducing ligand, NK cell type I receptor protein 2B4, SLAM family member 4, Signaling lymphocytic activation molecule 4, NAIL, NKR2B4, h2B4, SLAMF4
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Human CD244 ELISA Kit (2B4) is a Sandwich (quantitative) ELISA kit for the measurement of Human CD244 (2B4) in Human in Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 15.6 - 1000 pg/mL
- Assay time
- 3h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- < 10 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample 1 | n 16 | mean 0.45 ng/mL | SD 0.022 | C.V. 4.9 |
Sample Sample 2 | n 16 | mean 1.2 ng/mL | SD 0.076 | C.V. 6.3 |
Sample Sample 3 | n 16 | mean 3.2 ng/mL | SD 0.186 | C.V. 5.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample 1 | n 24 | mean 0.43 ng/mL | SD 0.027 | C.V. 6.2 |
Sample Sample 2 | n 24 | mean 1.3 ng/mL | SD 0.098 | C.V. 7.5 |
Sample Sample 3 | n 24 | mean 2.9 ng/mL | SD 0.194 | C.V. 6.7 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
The Human CD244 Enzyme-Linked Immunosorbent Assay (ELISA) kit (2B4) (ab213758) is designed for the quantitative measurement of Human CD244 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for CD244/2B4 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: C22-R221) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD244/2B4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CD244/2B4 amount of sample captured in plate.
CD244 (Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. Members of the CD2 family adhere to each other instead, the cell surface glycoprotein 2B4 is related to CD2 and is implicated in the regulation of NK and T-cell function. The 2B4 gene was mapped to 1q22 on gene map, where SLAM, CD48, CD84 and LY9 are also located. Using recombinant human NK cell-activating ligand 2B4 fused to domains 3 and 4 of rodent Cd4 and flow cytometric analysis, It was found that CD48 binds to 2B4. BIAcore surface plasmon resonance analysis showed that the affinity of CD48 for 2B4 is approximately 10-fold higher than that shown for CD48 and CD2.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
2B4 also known as CD244 or SLAMF4 is a cell surface receptor with a molecular mass of approximately 52-70 kDa. It exists predominantly on various immune cells like natural killer (NK) cells T cells basophils and monocytes. This receptor belongs to the signaling lymphocytic activation molecule (SLAM) family. 2B4's primary role is contributive during cell communication. It specifically recognizes CD48 on neighboring cells and modulates immune responses based on target cell recognition. This function empowers NK cells to maintain immune surveillance and cytotoxicity.
- Biological function summary
2B4 plays an integrative role within cellular immune response. When engaged by its ligand CD48 it regulates activation signals in NK cells and T cells. 2B4 is not a standalone entity but functions as a part of a larger cluster of differentiation molecules. This cluster including CD244.1 and CD244.2 facilitates signal transduction leading to immune modulation. The receptor acts as either an activating or inhibitory agent depending on the expression levels of its associated signaling proteins like SAP (SLAM-associated protein).
- Pathways
Cross-linking of 2B4 forms a critical axis in multiple immune pathways such as the NK cell-mediated cytotoxicity and adaptive immune response pathway. It interacts closely with SAP a protein that modulates downstream signaling events leading to immune cell activation or suppression. Besides SAP 2B4 collaboratively participates with other SLAM family receptors. These interactions optimize the balance between immune activation and tolerance ensuring appropriate immune responses.
- Associated diseases and disorders
2B4 has a significant involvement. Aberrant 2B4 signaling can lead to immune dysregulation linked to diseases like hemophagocytic lymphohistiocytosis (HLH) and certain autoimmune diseases. In HLH defective interactions between 2B4 and SAP contribute to impaired apoptosis of target cells leading to excessive immune proliferation. This dysfunctionality often features alongside compromised activity of related proteins such as perforin which is essential for target cell lysis. Consequently understanding 2B4's function and interaction helps in exploring targeted therapies for immune-associated disorders.
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Sandwich ELISA - Human CD244 ELISA Kit (2B4) (ab213758)
Human CD244 ELISA Kit (2B4) (ab213758) Standard Curve.
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