Human CD27 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CD27 in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
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Key facts
- Detection method
Colorimetric
- Sample types
Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
35.156 - 2250 pg/mL
- Assay time
1h 30m
- Sensitivity
= 12.969 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
Alternative names
CD27 antigen, CD27L receptor, T-cell activation antigen CD27, T14, Tumor necrosis factor receptor superfamily member 7, CD27, TNFRSF7
What's included?
Recommended products
Human CD27 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CD27 in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Key facts
- Detection method
Colorimetric
- Sample types
Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
35.156 - 2250 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 12.969 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 8.22 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 95 | Range 87 - 99 % |
Sample type Serum | Average % = 93 | Range 85 - 99 % |
Sample type EDTA Plasma | Average % = 96 | Range 89 - 101 % |
Sample type Heparin Plasma | Average % = 90 | Range 86 - 97 % |
Sample type Citrate plasma | Average % = 91 | Range 80 - 99 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human CD27 ELISA Kit (ab289648) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CD27 in serum, plasma (heparin), plasma (EDTA), plasma (citrate), and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD27 with 12.969 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD27 also known as Tumor Necrosis Factor Receptor Superfamily Member 7 (TNFRSF7) is a protein chiefly involved in T cell activation and proliferation. It belongs to the TNF-receptor superfamily and weighs approximately 50 kDa. CD27 is expressed mainly on T lymphocytes and to a lesser extent on B cells and natural killer cells. Researchers often study CD27 and its ligand CD70 to understand immune responses. Tools such as anti-CD27 percp 7h21 monoclonal antibodies and APC-conjugated forms help detect this protein in various assays.
- Biological function summary
One finds CD27 at the heart of cellular immune responses. It does not function alone; instead it typically forms part of a receptor-ligand complex with CD70 which influences T cell and B cell activities. This interaction promotes cell survival and plays a role in the activation of NF-kB and MAPK8/JNK signaling pathways which are essential for immune response regulation. These pathways ensure that the body's immune cells can efficiently respond to pathogens.
- Pathways
CD27 actively integrates into the NF-kB and mTOR signaling pathways which are important for regulating immune and inflammatory responses. Through these pathways CD27 works closely with proteins like TRAF2 and TRAF3 which are adapter molecules that transmit signals from the receptor. These signaling pathways provide a framework for understanding how CD27 and associated proteins work together to control immune cell behavior facilitating coordinated immune defense mechanisms.
- Associated diseases and disorders
CD27 has significant implications in autoimmune diseases and cancer. For example aberrant activation of CD27 may contribute to conditions like lymphoma and autoimmune diseases such as lupus. The CD70-CD27 signaling axis when dysregulated can lead to uncontrolled cell proliferation or apoptosis linking it to these pathologies. Understanding the interplay between CD27 and proteins like Bcl-2 which influences apoptosis helps pave the way for therapeutic strategies targeting these diseases.
Product promise
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2 product images
Sandwich ELISA - Human CD27 ELISA Kit (ab289648)
Interpolated concentrations of Human CD27 in serum, plasma (citrate), plasma (EDTA), plasma (heparin) and stimulated PBMC cell culture supernatant.
Interpolated concentration of native CD27 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 5%, plasma (citrate) 5%, plasma (EDTA) 5%, plasma (heparin) 5%, 1.5% PHA-M stimulated PBMC cell culture supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human CD27 ELISA Kit (ab289648)
Example of Human CD27 standard curve in Sample Diluent NS.
Example of Human CD27 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
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