AB221831

Human CD276 ELISA Kit

Recombinant
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(1 Publication)

Human CD276 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CD276 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD276, B7H3, PSEC0249, UNQ309/PRO352, CD276 antigen, 4Ig-B7-H3, B7 homolog 3, Costimulatory molecule, B7-H3

3 Images

ELISA - Human CD276 ELISA Kit (AB221831)

ELISA

Supplier Data

ELISA - Human CD276 ELISA Kit (AB221831)

Interpolated concentrations of CD276 in human serum and plasma samples. The concentrations of CD276 were measured in duplicates, interpolated from the CD276 standard curves and corrected for sample dilution. Undiluted samples : serum 50%, plasma (EDTA) 25%, plasma (citrate) 25%, plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD276 concentration was determined to be 31.7 ng/mL in serum, 25.0 ng/mL in plasma (EDTA), 25.1 ng/mL in plasma (citrate) and 28.5 ng/mL in plasma (heparin).

ELISA

Supplier Data

ELISA - Human CD276 ELISA Kit (AB221831)

Concentrations of CD276 in human serum samples.

Serum from ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD276 concentration was determined to be 31.5 ng/mL with a range of 18.7-53.6 ng/mL.

ELISA

Supplier Data

ELISA - Human CD276 ELISA Kit (AB221831)

Example of human CD276 standard curve in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 178 pg/mL

Range

578 - 37000 pg/mL

Assay time

2h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human CD276 ELISA Kit (ab221831) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD276 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA^® technology. Quantitate Human CD276 with 178 pg/ml sensitivity.

SimpleStep ELISA^® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details. Our SimpleStep ELISA^® technology provides several benefits:

- Single-wash protocol
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA^® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA^® kits.

CD276 (B7-H3) is a glycoprotein member of the B7 family, Ig superfamily of proteins. CD276 is involved in the regulation of T-cell-mediated immune response and is expressed by a variety of cell types.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.9" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "83 - 101 %", "average": "= 94" }, { "sample": "Serum", "range": "100 - 104 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "87 - 94 %", "average": "= 91" }, { "sample": "Urine", "range": "88 - 91 %", "average": "= 89" }, { "sample": "Heparin Plasma", "range": "85 - 89 %", "average": "= 87" }, { "sample": "Citrate plasma", "range": "90 - 100 %", "average": "= 94" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD276 also known as B7-H3 is an immunoregulatory protein with a mass of approximately 57 kDa although post-translational modifications can increase its observed size. It is a member of the B7 family which is part of the immunoglobulin superfamily. CD276 is widely expressed in various tissues including placenta liver heart and also in many tumor cells. It is observed in lesser amounts in normal tissues making it an interesting target for cancer research. Various assays like HEK293 and HEK293T cell confluency studies often use CD276 to explore its expression under different conditions and seeding densities which are represented in confluency charts.

Biological function summary

CD276 modulates immune responses by acting as a co-inhibitory ligand impacting T-cell proliferation and cytokine synthesis. Not part of a known complex it plays a role in the adaptive immune system by providing negative feedback regulation that can inhibit the immune response. This capability has implications in preventing autoimmunity but may also contribute to tumor immune evasion by reducing effective anti-tumor responses. The B7-H3/CD276 interaction with receptors such as IL receptor family members suggests its diverse immunomodulatory functions.

Pathways

CD276 participates in the T-cell receptor (TCR) signaling pathways influencing immune checkpoint pathways. It interacts with other immune checkpoint proteins like PD-L1 and CTLA-4 but its exact receptor partner remains unidentified. The presence of CD276 in these pathways highlights its role in controlling T-cell activation and tolerance. By modulating TCR signals and impacting downstream effects CD276 serves as a regulatory point that can balance immune activation and inhibition critical in immune-mediated conditions.

CD276 has strong associations with cancer particularly in solid tumors such as prostate and breast cancer. It is often overexpressed in malignant tissues contributing to immune evasion by tumors. Researchers propose it as a potential target for cancer immunotherapy due to this attribute. CD276's link to disorders like asthma has also been suggested where its interaction with proteins involved in inflammatory pathways such as IL response elements can exacerbate the condition. This implicates it as a dual-role protein in both disease progression and potential therapeutic targeting.

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Product protocols

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Target data

May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.

See full target information CD276

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation research 17:1577-1587 PubMed38495342

2024

The Impact of Laparoscopic Appendectomy and Open Appendectomy on B7-H3-Mediated Intrinsic Immune Response in Children with Acute Suppurative Appendicitis.

Applications

Unspecified application

Species

Unspecified reactive species

Chunsheng Duan,Yuan Li,Jiansu Ma,Yanglin Song,Lixia Zhou

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human CD276 ELISA Kit

ELISA - Human CD276 ELISA Kit (AB221831)

ELISA - Human CD276 ELISA Kit (AB221831)

ELISA - Human CD276 ELISA Kit (AB221831)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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