Human CD3 Epsilon ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD3 Epsilon with a sensitivity of 38.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Serum, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 84 - 5400 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 38.1 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128). In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (PubMed:38614099).
Alternative names
CD3e, T3E, CD3E, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain
What's included?
Recommended products
Human CD3 Epsilon ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD3 Epsilon with a sensitivity of 38.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Serum, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 84 - 5400 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 38.1 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 2.7 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 4 | mean - | SD - | C.V. 6.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 89 | Range 83 - 101 % |
Sample type Serum | Average % = 115 | Range 114 - 116 % |
Sample type Cell Lysate | Average % = 110 | Range 105 - 120 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human CD3 Epsilon ELISA kit (ab277708) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CD3 Epsilon in human serum, cell culture supernatant, and cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD3 Epsilon with 38.1 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
T-Cell Surface Glycoprotein CD3 epsilon chain (CD3e), also known as T-cell surface antigen T3/Leu-4 epsilon is a type I membrane protein encoded by the CD3E gene. CD3e forms the T-cell receptor (TCR)-CD3 complex on T-lymphocyte cell surfaces by forming two heterodimers CD3E/CD3D (delta) and CD3E/CD3G (gamma). This complex plays a role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3e contains an immunoreceptor tyrosine-based activation motif (ITAM) in the cytoplasmic domain. When engaged by TCR, these motifs are phosphorylated by Src protein family kinases LCK and FYN, which activate downstream signaling pathways.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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3 product images
Sandwich ELISA - Human CD3 Epsilon ELISA Kit (ab277708)
Example of human CD3 epsilon standard curve in Sample Diluent NS.
The CD3 epsilon standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human CD3 Epsilon ELISA Kit (ab277708)
Example of human CD3 epsilon standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.
The CD3 epsilon standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human CD3 Epsilon ELISA Kit (ab277708)
The CD3 epsilon standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of CD3 epsilon were measured in duplicate and interpolated from the CD3 epsilon standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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