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AB236711

Human CD30 ELISA Kit

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(1 Publication)

Human CD30 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD30 with a sensitivity of 6.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD30, D1S166E, TNFRSF8, Tumor necrosis factor receptor superfamily member 8, CD30L receptor, Ki-1 antigen, Lymphocyte activation antigen CD30

5 Images
ELISA - Human CD30 ELISA Kit (AB236711)
  • ELISA

Supplier Data

ELISA - Human CD30 ELISA Kit (AB236711)
Sandwich ELISA - Human CD30 ELISA Kit (AB236711)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD30 ELISA Kit (AB236711)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD30 concentration was determined to be 398.1 pg/mL with a range of 170.5 – 597.9 pg/mL.

Sandwich ELISA - Human CD30 ELISA Kit (AB236711)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD30 ELISA Kit (AB236711)

Interpolated concentrations of native CD30 in human cell culture supernatant samples.

The concentrations of CD30 were measured in duplicates, interpolated from the CD30 standard curves and corrected for sample dilution. Undiluted samples are as follows : HDLM-2 supernatant 6.3%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD30 concentration was determined to be 23.1 ng/mL in HDLM-2 supernatant.

Sandwich ELISA - Human CD30 ELISA Kit (AB236711)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD30 ELISA Kit (AB236711)

Interpolated concentrations of native CD30 in human serum and plasma samples.

The concentrations of CD30 were measured in duplicates, interpolated from the CD30 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (heparin) 50%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD30 concentration was determined to be 535.6 pg/ml in serum, 395.3 pg/ml in plasma (citrate), 434.5 pg/ml in plasma (heparin), and 512.9 pg/ml in plasma (EDTA).

Sandwich ELISA - Human CD30 ELISA Kit (AB236711)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD30 ELISA Kit (AB236711)

Example of human CD30 standard curve in Sample Diluent NS.

The CD30 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 6.4 pg/mL

Range

28 - 1800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CD30 ELISA Kit ab236711 is a rapid single-wash 90-min Sandwich ELISA to measure Human CD30 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 6.4 pg/mL.

How the assay works

Human CD30 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human CD30 ELISA Kit ab236711 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-CD30 antibody [EPR21828-36] - BSA and Azide free (Capture) ab245058
- Anti-CD30 antibody [EPR21828-217] - BSA and Azide free (Detector) ab245058
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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.9" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "96 - 105 %", "average": "= 99" }, { "sample": "Serum", "range": "90 - 91 %", "average": "= 90" }, { "sample": "EDTA Plasma", "range": "88 - 92 %", "average": "= 90" }, { "sample": "Heparin Plasma", "range": "86 - 98 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "83 - 91 %", "average": "= 86" }, { "sample": "Cell culture media", "range": "85 - 88 %", "average": "= 87" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab236711-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB236711-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB236711-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB236711-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD30 Capture Antibody", "number":"AB236711-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CD30 Lyophilized Recombinant Protein", "number":"AB236711-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB236711-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD30 Detector Antibody", "number":"AB236711-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB236711-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB236711-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB236711-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Receptor for TNFSF8/CD30L (PubMed : 8391931). May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B (PubMed : 8999898).
See full target information TNFRSF8
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer immunology, immunotherapy : CII 70:2353-2365 PubMed33527196

2021

CD30OX40 Treg is associated with improved overall survival in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Hang Lam,Michelle Hong,Si-Lin Koo,Clarinda Wei Ling Chua,Kah Ling Lim,Felicia Wee,Wei Keat Wan,Wei Qiang Leow,Joo Guan Yeo,Iain Bee Huat Tan,Joe Yeong,Tony Kiat Hon Lim,Tong Seng Lim
View all publications
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websiteProtocolBooklet
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