Human CD30 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CD30 in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
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- Cancer immunology, immunotherapy : CII 70:2353-23652021CD30OX40 Treg is associated with improved overall survival in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Hang Lam,Michelle Hong,Si-Lin Koo,Clarinda Wei Ling Chua,Kah Ling Lim,Felicia Wee,Wei Keat Wan,Wei Qiang Leow,Joo Guan Yeo,Iain Bee Huat Tan,Joe Yeong,Tony Kiat Hon Lim,Tong Seng LimPubMed 33527196
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 28 - 1800 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 6.4 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Receptor for TNFSF8/CD30L (PubMed:8391931). May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B (PubMed:8999898).
Alternative names
CD30, D1S166E, TNFRSF8, Tumor necrosis factor receptor superfamily member 8, CD30L receptor, Ki-1 antigen, Lymphocyte activation antigen CD30
What's included?
Recommended products
Human CD30 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CD30 in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 28 - 1800 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 6.4 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 5 | mean - | SD - | C.V. 3.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 3 | mean - | SD - | C.V. 2.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 99 | Range 96 - 105 % |
Sample type Serum | Average % = 90 | Range 90 - 91 % |
Sample type EDTA Plasma | Average % = 90 | Range 88 - 92 % |
Sample type Heparin Plasma | Average % = 90 | Range 86 - 98 % |
Sample type Citrate plasma | Average % = 86 | Range 83 - 91 % |
Sample type Cell culture media | Average % = 87 | Range 85 - 88 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human CD30 ELISA Kit (ab236711) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD30 protein in cit plasma, edta plasma, hep plasma, serum, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD30 with 6.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Sandwich ELISA - Human CD30 ELISA Kit (ab236711)
Example of human CD30 standard curve in Sample Diluent NS.
The CD30 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human CD30 ELISA Kit (ab236711)
Interpolated concentrations of native CD30 in human serum and plasma samples.
The concentrations of CD30 were measured in duplicates, interpolated from the CD30 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (heparin) 50%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD30 concentration was determined to be 535.6 pg/ml in serum, 395.3 pg/ml in plasma (citrate), 434.5 pg/ml in plasma (heparin), and 512.9 pg/ml in plasma (EDTA).
Sandwich ELISA - Human CD30 ELISA Kit (ab236711)
Interpolated concentrations of native CD30 in human cell culture supernatant samples.
The concentrations of CD30 were measured in duplicates, interpolated from the CD30 standard curves and corrected for sample dilution. Undiluted samples are as follows: HDLM-2 supernatant 6.3%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD30 concentration was determined to be 23.1 ng/mL in HDLM-2 supernatant.
Sandwich ELISA - Human CD30 ELISA Kit (ab236711)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD30 concentration was determined to be 398.1 pg/mL with a range of 170.5 – 597.9 pg/mL.
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