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Human CD300e ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human CD300e in Human in Cell culture supernatant, Plasma, Serum samples.

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Images

Sandwich ELISA - Human CD300e ELISA Kit (AB313525), expandable thumbnail

Key facts

Detection method
Colorimetric
Sample types
Cell culture supernatant, Plasma, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
0.5 - 30 ng/mL
Sensitivity
= 0.5 ng/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Probably acts as an activating receptor.

Alternative names

CD300e, CD300LE, CLM2, CMRF35A5, IREM2, CD300E, CMRF35-like molecule 2, CLM-2, CD300 antigen-like family member E, CMRF35-A5, Immune receptor expressed on myeloid cells 2, Polymeric immunoglobulin receptor 2, IREM-2, PIgR-2, PIgR2, Poly-Ig receptor 2

What's included?

1 x 96 Tests
Components
100X HRP-Streptavidin
1 x 200 µL
20X Wash Buffer
1 x 25 mL
5X Assay Diluent B (Item E)
1 x 15 mL
5X Assay Diluent D (Item K)
1 x 15 mL
Biotinylated Human CD300e Detection Antibody
2 x 1 Vial
Human CD300e Antibody-coated ELISA Plate
1 x 1 Unit
Lyophilized Human CD300e Protein Standard
2 x 1 Vial
Stop Solution; 0.2M Sulfuric Acid
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

Recommended products

Human CD300e ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human CD300e in Human in Cell culture supernatant, Plasma, Serum samples.

Key facts

Detection method
Colorimetric
Sample types
Cell culture supernatant, Plasma, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
0.5 - 30 ng/mL
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
= 0.5 ng/mL

Recovery

Sample specific recovery

Sample type
Serum
Average %
= 93.21
Range
85 - 107 %
Sample type
Plasma
Average %
= 99.35
Range
89 - 113 %
Sample type
Cell culture media
Average %
= 112.2
Range
97 - 141 %

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Human CD300e ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD300e in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human CD300e coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Activin RIA, ASAHL, B4GalT1, BAI1, Brorin, C1qTNF4, CA14, CA4, CA6, CA8, Cadherin-6, Caspr2, CD27 Ligand, CD300a, CD300f, CD4, CD5, CD69, CK18, CK19, CPB1, CRISP-2, DDR1, FUT8, MIA, NTAL, NTB-A, OMgp, PEAR1, Podoplanin, PTH1R, Reg4, ROR1, Semaphorin 4G, Serpin A5, Serpin B6, Siglec-1, Sirtuin 2, Sirtuin 5.

Product promise

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1 product image

  • Sandwich ELISA - Human CD300e ELISA Kit (ab313525), expandable thumbnail

    Sandwich ELISA - Human CD300e ELISA Kit (ab313525)

    Example of standard curve using ab313525. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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