Human CD31 ELISA Kit (PECAM)
- Recombinant
- SimpleStep
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(2 Publications)
Human CD31 ELISA Kit (PECAM) is a single-wash 90-min Simplestep used to quantify Human CD31 (PECAM) with a sensitivity of 44 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
CD31, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1
- sELISA
Supplier Data
Sandwich ELISA - Human CD31 ELISA Kit (PECAM) (AB190814)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD31 concentration was determined to be 6.34 ng/mL with a range of 4.39 – 11.1 ng/mL
- sELISA
Supplier Data
Sandwich ELISA - Human CD31 ELISA Kit (PECAM) (AB190814)
Interpolated concentrations of native CD31 in human serum and plasma samples.
The concentrations of CD31 were measured in duplicates, interpolated from the CD31 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 36%, plasma (citrate) 9%, plasma (heparin) 9% and plasma (EDTA) 9%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD31 concentration was determined to be 6.60 ng/mL in neat serum, 5.04 ng/mL in neat plasma (citrate), 5.67 ng/mL in neat plasma (heparin), and 6.44 ng/mL in neat plasma (EDTA).
- sELISA
Supplier Data
Sandwich ELISA - Human CD31 ELISA Kit (PECAM) (AB190814)
Human CD31 Standard Curve Comparison.
Standard Curve comparison between human CD31 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
- sELISA
Supplier Data
Sandwich ELISA - Human CD31 ELISA Kit (PECAM) (AB190814)
Human CD31 Standard Curve Comparison.
Standard Curve comparison between human CD31 new SimpleStep ELISA kit and previous ELISA kit. The new SimpleStep ELISA kit shows increased sensitivity.
- sELISA
Supplier Data
Sandwich ELISA - Human CD31 ELISA Kit (PECAM) (AB190814)
Example of human CD31 standard curve in 1X Cell Extraction Buffer PTR + Enhancer.
The CD31 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human CD31 ELISA Kit (PECAM) (AB190814)
Interpolated concentrations of native CD31 in THP-1 cell extract samples based on a 125 μg/mL extract load.
THP-1 cells were cultured in the presence (stimulated) or absence (unstimulated) of 5 μg/mL LPS and 50nM PMA for 24 hours. The concentrations of CD31 were measured in duplicate and interpolated from the CD31 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD31 concentration was determined to be 16.0 ng/mL in stimulated THP-1 cell extract and 12.5 ng/mL in unstimulated THP-1 cell extract.
- sELISA
Supplier Data
Sandwich ELISA - Human CD31 ELISA Kit (PECAM) (AB190814)
Example of human CD31 standard curve in Sample Diluent NS + Enhancer.
The CD31 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human CD31 ELISA Kit (PECAM) (AB190814)
Interpolated concentrations of native CD31 in human saliva, urine, and THP-1 cell culture supernatant samples.
THP-1 cells were cultured in the presence (stimulated) or absence (mock) of 5 μg/mL LPS and 50nM PMA for 24 hours. The concentrations of CD31 were measured in duplicates, interpolated from the CD31 standard curves and corrected for sample dilution. Undiluted samples are as follows : saliva 36%, urine 36%, THP-1 stimulated supernatant 18%, and THP-1 mock supernatant 18%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD31 concentration was determined to be 6.85 ng/mL in neat saliva, 0.81 ng/mL in neat urine, 2.27 ng/mL in neat stimulated THP-1 supernatant, and 0.64 ng/mL in neat mock THP-1 supernatant.
Reactivity data
Product details
Human CD31 ELISA Kit (PECAM) (ab190814) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD31 (PECAM) protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, saliva, serum, tissue extracts, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD31 (PECAM) with 44 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
CD31, also known as PECAM-1, is a cell adhesion molecule which is required for leukocyte trans-endothelial migration (TEM) under most inflammatory conditions. CD31 is a 130-kDa glycoprotein constitutively expressed on endothelial cells, platelets, neutrophils, monocytes, lymphocytes and basophils. CD31 molecules is expressed in large amounts in the intracellular junctions of endothelial cells. Experiments using antibody results in blocking CD31 homophilic interaction and inhibition of leukocytes transmigration. Expression of CD31 on the cell surface is regulated by receptor shedding resulting in generation of a soluble 100-kDa extracellular domain form and a truncated 28-kDa fragment containing the transmembrane and cytoplasmic domain of CD31.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Publications (2)
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Molecular therapy. Nucleic acids 24:528-541 PubMed33898105
2021
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Journal of cancer research and clinical oncology 145:411-427 PubMed30483898
2018
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