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AB45910

Human CD31 ELISA Kit (PECAM1)

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(1 Publication)

Human CD31 ELISA Kit (PECAM1) is a Sandwich ELISA for the measurement of Human CD31 (PECAM1) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD31, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1

1 Images
Sandwich ELISA - Human CD31 ELISA Kit (PECAM1) (AB45910)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD31 ELISA Kit (PECAM1) (AB45910)

Representative Standard Curve using ab45910.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 0.04 ng/mL

Range

0.31 - 10 ng/mL

Assay time

2h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's CD31 (PECAM1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD31 in Human serum, plasma, buffered solutions and cell culture medium

A monoclonal antibody specific for CD31 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD31 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD31 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD31 present in the samples.

This kit will recognize both endogenous and recombinant Human CD31.

Get results in 90 minutes with Human CD31 ELISA Kit (PECAM) (ab190814) from our SimpleStep ELISA® range.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 11, "mean": "5.406 ng/mL", "standardDeviation": "0.292", "coefficientOfVariability": "5.4" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 11, "mean": "1.205 ng/mL", "standardDeviation": "0.058", "coefficientOfVariability": "4.8" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 11, "mean": "5.323 ng/mL", "standardDeviation": "0.19", "coefficientOfVariability": "3.5" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 11, "mean": "1.236 ng/mL", "standardDeviation": "0.077", "coefficientOfVariability": "6.2" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed : 17580308, PubMed : 19342684). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed : 19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed : 27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed : 17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed : 12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed : 12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed : 18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed : 18672896). Induces susceptibility to atherosclerosis (By similarity).. Isoform Delta15. Does not protect against apoptosis.
See full target information PECAM1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PLoS computational biology 6: PubMed20885780

2010

Differentially expressed RNA from public microarray data identifies serum protein biomarkers for cross-organ transplant rejection and other conditions.

Applications

ELISA

Species

Human

Rong Chen,Tara K Sigdel,Li Li,Neeraja Kambham,Joel T Dudley,Szu-Chuan Hsieh,R Bryan Klassen,Amery Chen,Tuyen Caohuu,Alexander A Morgan,Hannah A Valantine,Kiran K Khush,Minnie M Sarwal,Atul J Butte
View all publications
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Product promise

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