Human CD31 ELISA Kit (PECAM1) is a Sandwich (quantitative) ELISA kit for the measurement of Human CD31 (PECAM1) in Human in Plasma, Cell culture supernatant, Serum samples.
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- PLoS computational biology 6:2010Differentially expressed RNA from public microarray data identifies serum protein biomarkers for cross-organ transplant rejection and other conditions.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.31 - 10 ng/mL
- Assay time
- 2h 45m
- Sensitivity
- < 0.04 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:17580308, PubMed:19342684). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis (By similarity). Isoform Delta15. Does not protect against apoptosis.
Alternative names
CD31, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1
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Human CD31 ELISA Kit (PECAM1) is a Sandwich (quantitative) ELISA kit for the measurement of Human CD31 (PECAM1) in Human in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.31 - 10 ng/mL
- Assay time
- 2h 45m
- Assay Platform
- Microplate
- Sensitivity
- < 0.04 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample A | n 11 | mean 5.323 ng/mL | SD 0.19 | C.V. 3.5 |
Sample Sample B | n 11 | mean 1.236 ng/mL | SD 0.077 | C.V. 6.2 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample A | n 11 | mean 5.406 ng/mL | SD 0.292 | C.V. 5.4 |
Sample Sample B | n 11 | mean 1.205 ng/mL | SD 0.058 | C.V. 4.8 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Abcam's CD31 (PECAM1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD31 in Human serum, plasma, buffered solutions and cell culture medium
A monoclonal antibody specific for CD31 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD31 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD31 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD31 present in the samples.
This kit will recognize both endogenous and recombinant Human CD31.
Get results in 90 minutes with Human CD31 ELISA Kit (PECAM) (Human CD31 ELISA Kit (PECAM) ab190814) from our SimpleStep ELISA® range.
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Terms & Conditions.
1 product image
Sandwich ELISA - Human CD31 ELISA Kit (PECAM1) (ab45910)
Representative Standard Curve using ab45910.
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Product protocols
- Visit the general protocols
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