JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB314830

Human CD314 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human CD314 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CD314 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD314, D12S2489E, NKG2D, KLRK1, NKG2-D type II integral membrane protein, Killer cell lectin-like receptor subfamily K member 1, NK cell receptor D, NKG2-D-activating NK receptor

1 Images
Sandwich ELISA - Human CD314 ELISA Kit (AB314830)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD314 ELISA Kit (AB314830)

Example of standard curve using ab314830. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.4 ng/mL

Range

0.4 - 100 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human CD314 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD314 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human CD314 coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Adenosine Deaminase/ADA, AIF, AKR1C4 , ASAH2, BCL-2, BID, Calreticulin, Calreticulin-2, CD39L3/ENTPD3, CD51, CD99-L2, CDC25B, Cerberus 1, CHST2, Cochlin, CRELD2, DCSIGNR/CD299, eNOS, ENPP2/Autotaxin, FABP4, Fc epsilon RI, FGF R5, GALNT2, GALNT3, GIF, GPR111, beta-Glucuronidase/GUSB, Inhibin A, LILRB4/CD85k/ILT3, Neuroglycan C/CSPG5, NKp46/NCR1, Neuronal Pentraxin R/NPTXR, ROR2, SCCA2, Siglec2/CD22, SIRP alpha/CD172a, SorCS1, Trypsin 1/PRSS1, Trypsin 3/PRSS3.

style
left-column

Recovery

[ { "sample": "Serum", "range": "75 - 82 %", "average": "= 77.9" }, { "sample": "Plasma", "range": "70 - 83 %", "average": "= 76.4" }, { "sample": "Cell culture media", "range": "110 - 123 %", "average": "= 116.7" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314830-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin", "number":"AB314830-CMP03" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human CD314 Detection Antibody", "number":"AB314830-CMP09" }, { "size":"1 x 1 Unit", "name":"Human CD314 Antibody-coated ELISA Plate", "number":"AB314830-CMP08" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314830-CMP04" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314830-CMP05" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human CD314 Protein Standard", "number":"AB314830-CMP10" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314830-CMP06" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314830-CMP07" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314830-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314830-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"ELISA Microplate Bag", "number":"AB314830-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"96-Well Plate Sealing Film", "number":"AB314830-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin", "number":"AB314830-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314830-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314830-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314830-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human CD314 Antibody-coated ELISA Plate", "number":"AB314830-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human CD314 Detection Antibody", "number":"AB314830-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human CD314 Protein Standard", "number":"AB314830-CMP10", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NKG2D also known as natural killer group 2 member D is a type-2 transmembrane receptor with a mass of approximately 42 kDa. Expressed primarily on natural killer (NK) cells CD8+ T cells subsets of CD4+ T cells and some macrophages it recognizes stress-induced ligands presented on target cells. This protein plays an important role in the immune system's recognition and elimination of infected or transformed cells by binding to its ligands which include MHC class I chain-related proteins MICA/B and UL16-binding proteins.
Biological function summary

NKG2D acts as an activating receptor on the cell surface that initiates immune responses leading to cytotoxicity against target cells. It functions as part of a complex involving the adaptor protein DAP10 which transduces activating signals to promote cellular immune responses. This interaction amplifies the activation of immune cells and contributes to the host's defense against tumors and virally infected cells.

Pathways

NKG2D plays a significant role in immune surveillance and signaling pathways. It actively involves in the natural killer (NK) cell-mediated cytotoxicity pathway and the adaptive immune response pathway. The interaction with proteins such as DAP10 is critical for its signaling function contributing to its interaction within these pathways. The signaling through NKG2D directly affects the activation states and responses of immune cells which are vital in immune defense.

NKG2D is implicated in cancer and autoimmune diseases. In cancer overexpression of its ligands on tumor cells makes them susceptible to NKG2D-mediated cell lysis. On the other hand aberrant expression of NKG2D ligands in autoimmune disorders can lead to inappropriate immune activation. The interaction with MICA/B proteins is significant in these conditions emphasizing its importance in both immune surveillance and potential disease pathogenesis.
style
left-column

Product protocols

style
left-column

Target data

Functions as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8(+) T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins including MICA, MICB, RAET1E, RAET1G, RAET1L/ULBP6, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4.
See full target information KLRK1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Primary Antibodies

AB302907

Anti-NKG2D antibody [EPR24072-342]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry - Anti-NKG2D antibody [EPR24072-342] (AB302907)

  • 0
  • 0
Proteins & Peptides

AB281815

Recombinant Human NKG2D protein

SDS-PAGE - Recombinant Human NKG2D protein (AB281815)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com