Human CD34 ELISA Kit- Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CD34 - Intracellular in Cell Lysate, Tissue Extracts samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 39.063 - 2500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 12.486 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Alternative names
CD34, Hematopoietic progenitor cell antigen CD34
What's included?
Human CD34 ELISA Kit- Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human CD34 - Intracellular in Cell Lysate, Tissue Extracts samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 39.063 - 2500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 12.486 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 8 | C.V. = 1.5 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 3 | C.V. = 2.1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell Lysate | Average % = 105 | Range 103 - 108 % |
Sample type Tissue Extracts | Average % = 99 | Range 91 - 112 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human CD34 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD34 protein in Cell Lysate, Tissue extracts. Quantitate Human CD34 with 12.486 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibodyanalyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Singlewash protocol reduces assay time to 90 minutes or less.
- High sensitivity, specificity and reproducibility from superior antibodies.
- Fully validated in biological samples.
- 96wells plate breakable into 12 x 8 wells strips, available in 22pack (10 x 96well plates).
- Also available in a fully validated 384well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Sandwich ELISA - Human CD34 ELISA Kit- Intracellular (ab323933)
Interpolated concentration of native CD34 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are TF-1 cell extract 40 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.
Sandwich ELISA - Human CD34 ELISA Kit- Intracellular (ab323933)
Interpolated concentration of native CD34 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: TF-1 cell extract 40 μg/mL and human kidney tissue extract 125 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.
Sandwich ELISA - Human CD34 ELISA Kit- Intracellular (ab323933)
Example of human CD34 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human CD34 ELISA Kit- Intracellular (ab323933)
Example of human CD34 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com