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AB323933

Human CD34 ELISA Kit- Intracellular

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Human CD34 ELISA Kit- Intracellular is a Sandwich ELISA for the measurement of Human CD34 - Intracellular in Human in Cell/Tissue Extracts samples.

View Alternative Names

CD34, Hematopoietic progenitor cell antigen CD34

4 Images
Sandwich ELISA - Human CD34 ELISA Kit- Intracellular (AB323933)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD34 ELISA Kit- Intracellular (AB323933)

Interpolated concentration of native CD34 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are TF-1 cell extract 40 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

Sandwich ELISA - Human CD34 ELISA Kit- Intracellular (AB323933)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD34 ELISA Kit- Intracellular (AB323933)

Interpolated concentration of native CD34 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : TF-1 cell extract 40 μg/mL and human kidney tissue extract 125 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW.

Sandwich ELISA - Human CD34 ELISA Kit- Intracellular (AB323933)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD34 ELISA Kit- Intracellular (AB323933)

Example of human CD34 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human CD34 ELISA Kit- Intracellular (AB323933)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD34 ELISA Kit- Intracellular (AB323933)

Example of human CD34 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 12.486 pg/mL

Range

39.063 - 2500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CD34 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD34 protein in Cell Lysate, Tissue extracts. Quantitate Human CD34 with 12.486 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibodyanalyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Singlewash protocol reduces assay time to 90 minutes or less.

- High sensitivity, specificity and reproducibility from superior antibodies.

- Fully validated in biological samples.

- 96wells plate breakable into 12 x 8 wells strips, available in 22pack (10 x 96well plates).

- Also available in a fully validated 384well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1.5" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.1" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "103 - 108 %", "average": "= 105" }, { "sample": "Tissue Extracts", "range": "91 - 112 %", "average": "= 99" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
See full target information CD34
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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