Human CD38 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD38 with a sensitivity of 7.44 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 31.25 - 2000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 7.44 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Synthesizes cyclic ADP-ribose (cADPR), a second messenger for glucose-induced insulin secretion (PubMed:7961800, PubMed:8253715). Synthesizes the Ca(2+) mobilizer nicotinate-adenine dinucleotide phosphate, NAADP(+), from 2'-phospho-cADPR and nicotinic acid, as well as from NADP(+) and nicotinic acid. At both pH 5.0 and pH 7.4 preferentially transforms 2'-phospho-cADPR into NAADP(+), while preferentially cleaving NADP(+) to cADPR and ADPRP rather than into NADDP(+) (PubMed:16690024). Has cADPR hydrolase activity (PubMed:7961800, PubMed:8253715).
Alternative names
CD38, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, 2'-phospho-ADP-ribosyl cyclase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-cyclic-ADP-ribose transferase, ADP-ribosyl cyclase 1, Cyclic ADP-ribose hydrolase 1, T10, ADPRC 1, cADPR hydrolase 1
What's included?
Recommended products
Human CD38 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD38 with a sensitivity of 7.44 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 31.25 - 2000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 7.44 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 5.2 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 7.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 107 | Range 98 - 117 % |
Sample type Serum | Average % = 85 | Range 82 - 88 % |
Sample type EDTA Plasma | Average % = 82 | Range 76 - 90 % |
Sample type Heparin Plasma | Average % = 88 | Range 85 - 90 % |
Sample type Citrate plasma | Average % = 91 | Range 90 - 93 % |
Sample type Cell Lysate | Average % = 87 | Range 75 - 94 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human CD38 ELISA Kit (ab267801) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD38 protein in cell culture supernatant, cell lysate, edta plasma, hep plasma, serum, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD38 with 7.44 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
CD38, also known as ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 (EC:3.2.2.6), is a single-pass type II membrane protein. CD38 catalyzes synthesis of cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. It also has a cyclic ADP-ribose hydrolase activity.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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5 product images
Sandwich ELISA - Human CD38 ELISA Kit (ab267801)
Example of human CD38 standard curve in Sample Diluent NS.
The CD38 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human CD38 ELISA Kit (ab267801)
Interpolated concentrations of native CD38 in human Daudi cell culture supernatant sample.
The concentrations of CD38 were measured in duplicates, interpolated from the CD38 standard curves and corrected for sample dilution. Undiluted samples are as follows: Daudi cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD38 concentration was determined to be 1,493 pg/mL in neat Daudi supernatant.
Sandwich ELISA - Human CD38 ELISA Kit (ab267801)
Interpolated concentrations of native CD38 in human serum, plasma-citrate, plasma-EDTA and plasma-heparin samples.
The concentrations of CD38 were measured in duplicates in neat samples and interpolated from the CD38 standard curves. The interpolated values are plotted (mean +/- SD, n=2). The mean CD38 concentration was determined to be 47.6 pg/mL in serum, 35.0 pg/mL in plasma-citrate, 51.4 pg/mL in plasma-EDTA and 39.5 pg/mL in plasma-heparin.
Sandwich ELISA - Human CD38 ELISA Kit (ab267801)
Interpolated concentrations of native CD38 in human Raji and Daudi cells based on a 5 μg/mL extract load.
The concentrations of CD38 were measured in duplicate and interpolated from the CD38 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD38 concentration was determined to be 1,268 pg/mL in Raji extract and 1,323 pg/mL in Daudi extract.
Sandwich ELISA - Human CD38 ELISA Kit (ab267801)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD38 concentration was determined to be 51.8 pg/mL with a range of 36.6 – 82.2 pg/mL.
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