Human CD38 ELISA Kit
- Recombinant
- SimpleStep
- What is this?
Be the first to review this product! Submit a review
|
(0 Publication)
Human CD38 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD38 with a sensitivity of 7.44 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
CD38, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, 2'-phospho-ADP-ribosyl cyclase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-cyclic-ADP-ribose transferase, ADP-ribosyl cyclase 1, Cyclic ADP-ribose hydrolase 1, T10, ADPRC 1, cADPR hydrolase 1
- sELISA
Supplier Data
Sandwich ELISA - Human CD38 ELISA Kit (AB267801)
Interpolated concentrations of native CD38 in human Daudi cell culture supernatant sample.
The concentrations of CD38 were measured in duplicates, interpolated from the CD38 standard curves and corrected for sample dilution. Undiluted samples are as follows : Daudi cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD38 concentration was determined to be 1,493 pg/mL in neat Daudi supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Human CD38 ELISA Kit (AB267801)
Interpolated concentrations of native CD38 in human serum, plasma-citrate, plasma-EDTA and plasma-heparin samples.
The concentrations of CD38 were measured in duplicates in neat samples and interpolated from the CD38 standard curves. The interpolated values are plotted (mean +/- SD, n=2). The mean CD38 concentration was determined to be 47.6 pg/mL in serum, 35.0 pg/mL in plasma-citrate, 51.4 pg/mL in plasma-EDTA and 39.5 pg/mL in plasma-heparin.
- sELISA
Supplier Data
Sandwich ELISA - Human CD38 ELISA Kit (AB267801)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD38 concentration was determined to be 51.8 pg/mL with a range of 36.6 – 82.2 pg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human CD38 ELISA Kit (AB267801)
Example of human CD38 standard curve in Sample Diluent NS.
The CD38 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human CD38 ELISA Kit (AB267801)
Interpolated concentrations of native CD38 in human Raji and Daudi cells based on a 5 μg/mL extract load.
The concentrations of CD38 were measured in duplicate and interpolated from the CD38 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD38 concentration was determined to be 1,268 pg/mL in Raji extract and 1,323 pg/mL in Daudi extract.
Reactivity data
Product details
Human CD38 ELISA Kit (ab267801) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD38 protein in cell culture supernatant, cell lysate, edta plasma, hep plasma, serum, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD38 with 7.44 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
CD38, also known as ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 (EC:3.2.2.6), is a single-pass type II membrane protein. CD38 catalyzes synthesis of cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. It also has a cyclic ADP-ribose hydrolase activity.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com