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AB99990

Human CD40 ELISA Kit

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(4 Publications)

Human CD40 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human CD40 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40

1 Images
Sandwich ELISA - Human CD40 ELISA Kit (AB99990)
  • sELISA

Supplier Data

Sandwich ELISA - Human CD40 ELISA Kit (AB99990)

Representative Standard Curve using ab99990

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 50 pg/mL

Range

54.87 - 40000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's CD40 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD40 in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human CD40 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "80 - 100 %", "average": "= 92.27" }, { "sample": "Serum", "range": "104 - 124 %", "average": "= 114.7" }, { "sample": "Plasma", "range": "78 - 100 %", "average": "= 90.19" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab99990-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB99990-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB99990-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB99990-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-human CD40", "number":"AB99990-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB99990-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CD40 Microplate (12 strips x 8 wells)", "number":"AB99990-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"CD40 Standard Human (recombinant) (lyophilized)", "number":"AB99990-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB99990-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB99990-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Receptor for TNFSF5/CD40LG (PubMed : 31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).
See full target information CD40
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

eLife 10: PubMed34854378

2021

Single-cell-level protein analysis revealing the roles of autoantigen-reactive B lymphocytes in autoimmune disease and the murine model.

Applications

Unspecified application

Species

Unspecified reactive species

Takemichi Fukasawa,Ayumi Yoshizaki,Satoshi Ebata,Asako Yoshizaki-Ogawa,Yoshihide Asano,Atsushi Enomoto,Kiyoshi Miyagawa,Yutaka Kazoe,Kazuma Mawatari,Takehiko Kitamori,Shinichi Sato

Frontiers in immunology 9:1789 PubMed30131806

2018

Extracellular Vesicles Work as a Functional Inflammatory Mediator Between Vascular Endothelial Cells and Immune Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Baharak Hosseinkhani,Sören Kuypers,Nynke M S van den Akker,Daniel G M Molin,Luc Michiels

Scientific reports 7:7942 PubMed28801616

2017

Circulating CD40 and sCD40L Predict Changes in Renal Function in Subjects with Chronic Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey X Xie,Helen Alderson,James Ritchie,Philip A Kalra,Yanmei Xie,Kaili Ren,Hanh Nguyen,Tian Chen,Pamela Brewster,Rajesh Gupta,Lance D Dworkin,Deepak Malhotra,Christopher J Cooper,Jiang Tian,Steven T Haller

Journal of hematology & oncology 7:64 PubMed25227919

2014

Highly efficient, in-vivo Fas-mediated apoptosis of B-cell lymphoma by hexameric CTLA4-FasL.

Applications

sELISA

Species

Human

Alexandra Aronin,Shira Amsili,Tatyana B Prigozhina,Kobi Tzdaka,Roy Shen,Leonid Grinmann,Fanny Szafer,Per Edebrink,Mari-Anne Rauvola,Noam Shani,Michal Dranitzki Elhalel
View all publications
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websiteProtocolBooklet
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Product promise

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