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Human CD40L ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human CD40L in Human in Plasma, Cell culture supernatant, Serum samples.

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Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

8.23 - 6000 pg/mL

Sensitivity

< 6 pg/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed:1280226, PubMed:31331973). Costimulates T-cell proliferation and cytokine production (PubMed:8617933). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B (PubMed:15067037, PubMed:31331973). Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed:15067037). Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity). Involved in immunoglobulin class switching (By similarity).CD40 ligand, soluble formActs as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.

Alternative names

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
5X Assay Diluent B
1 x 15 mL
600X HRP-Streptavidin Concentrate
1 x 200 µL
Assay Diluent A
1 x 30 mL
Biotinylated anti-Human CD40L
2 x 1 Vial
CD40L Microplate (12 x 8 well strips)
1 x 1 Unit
Recombinant Human CD40L Standard (lyophilized)
2 x 1 Vial
Stop Solution
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

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Human CD40L ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human CD40L in Human in Plasma, Cell culture supernatant, Serum samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

8.23 - 6000 pg/mL

Assay Platform

Microplate

Sensitivity

< 6 pg/mL

General recovery

95%

Recovery

Sample specific recovery

Sample type

Cell culture supernatant

Average %

= 96.35

Range

86 - 105 %

Sample type

Serum

Average %

= 93.57

Range

83 - 103 %

Sample type

Plasma

Average %

= 94.63

Range

84 - 105 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Human CD40L ELISA Kit (ab99991) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD40L in serum, plasma (EDTA or heparin), and cell culture supernatants.

This assay employs an antibody specific for Human CD40L coated on a 96-well plate. Standards and samples are pipetted into the wells and CD40L present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD40L antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD40L bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get results in 90 minutes with Human CD40 Ligand ELISA Kit (TNFSF5) (ab196268) from our SimpleStep ELISA® range.

Optimization may be required with urine samples.

Supplementary info

Biological function summary

The interaction between CD40L and CD40 triggers cell proliferation differentiation and apoptosis in immune cells. CD40L contributes to the formation of an immunological synapse encompassing its role in T helper cell function. Furthermore CD40L is implicated in the activation of B cells which are necessary for antibody class switching and memory cell generation. Its broad influence shows CD40L's involvement in immune system regulation and response.

Activity summary

TRAP/CD40L also known as CD154 or CD40 ligand is a type II transmembrane protein with a molecular mass of approximately 39 kDa. CD40L is mainly expressed on the surface of activated T cells. This protein binds to CD40 a significant receptor present on several immune cells including B cells macrophages and dendritic cells. The interaction with CD40 is essential for immune cell activation and development marking CD40L as an important player in adaptive immunity.

Pathways

CD40L is integral to the CD40 signaling pathway which is involved in immune and inflammatory responses. CD40L activates the NF-kB and JNK signaling pathways by engaging with CD40 which then drives the transcription of genes related to immune response. This signaling cascade impacts several other proteins such as TRAF proteins which act as intermediaries in propagating the signal.

Associated diseases and disorders

CD40L has been linked to immune-related conditions including autoimmune disorders and inflammatory diseases. Elevated CD40L expression is observed in diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis. In SLE CD40L interaction with CD40 on B cells is known to exacerbate disease severity by promoting autoantibody production. Exploring anti-CD40L therapeutic strategies can offer potential intervention in such diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Sandwich ELISA - Human CD40L ELISA Kit (ab99991), expandable thumbnail

    Sandwich ELISA - Human CD40L ELISA Kit (ab99991)

    Representative Standard Curve using ab99991.

  • Sandwich ELISA - Human CD40L ELISA Kit (ab99991), expandable thumbnail

    Sandwich ELISA - Human CD40L ELISA Kit (ab99991)

    Representative Standard Curve using ab99991.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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