AB324330

Human CD42b/GPIb alpha ELISA Kit

SimpleStep
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Human CD42b/GPIb alpha ELISA Kit is a Sandwich ELISA for the measurement of Human CD42b/GPIb alpha in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD42b, Platelet glycoprotein Ib alpha chain, GP-Ib alpha, GPIb-alpha, GPIbA, Glycoprotein Ibalpha, Antigen CD42b-alpha, GP1BA

6 Images

Sandwich ELISA - Human CD42b/GPIb alpha ELISA Kit (AB324330)

sELISA

Supplier Data

Sandwich ELISA - Human CD42b/GPIb alpha ELISA Kit (AB324330)

Example of human CD42b/GPIb alpha standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human CD42b/GPIb alpha ELISA Kit (AB324330)

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD42b/GPIb alpha concentration was determined to be 720.84 ng/mL with a range of 439.63 – 1,196.28 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human CD42b/GPIb alpha ELISA Kit (AB324330)

Interpolated concentration of native CD42b/GPIb alpha was measured in duplicate at different sample concentrations. Undiluted samples are as follows : PBMC cell culture supernatant 100%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human CD42b/GPIb alpha ELISA Kit (AB324330)

Interpolated concentration of native CD42b/GPIb alpha was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 100, plasma (citrate) 1 : 100, plasma (EDTA) 1 : 50, and plasma (heparin) 1 : 100. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human CD42b/GPIb alpha ELISA Kit (AB324330)

Interpolated concentration of native CD42b/GPIb alpha was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 100 human serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human CD42b/GPIb alpha ELISA Kit (AB324330)

Example of human CD42b/GPIb alpha standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 71.95 pg/mL

Range

187.5 - 12000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human CD42b/GPIb alpha SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD42b/GPIb alpha protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human CD42b/GPIb alpha with 71.95 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3" } ]

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Recovery

[ { "sample": "Serum", "range": "90 - 122 %", "average": "= 102" }, { "sample": "Citrate plasma", "range": "83 - 97 %", "average": "= 91" }, { "sample": "EDTA Plasma", "range": "93 - 111 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "107 - 117 %", "average": "= 113" }, { "sample": "Cell culture supernatant", "range": "81 - 99 %", "average": "= 92" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium.

See full target information GP1BA

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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