AB287168

Human CD44 ELISA Kit

Recombinant
SimpleStep
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Human CD44 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human CD44 with a sensitivity of 12.28 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD44, LHR, MDU2, MDU3, MIC4, CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, ECMR-III, PGP-1, PGP-I

5 Images

ELISA

Supplier Data

ELISA - Human CD44 ELISA Kit (AB287168)

sELISA

Supplier Data

Sandwich ELISA - Human CD44 ELISA Kit (AB287168)

Interpolated concentrations of human CD44 in uterus tissue extract.

Interpolated concentration of native CD44 was measured in duplicate at different sample concentrations. Undiluted samples are 3.13 ug/mL for uterus tissue extract samples. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human CD44 ELISA Kit (AB287168)

Example of human CD44 standard curve in 1X Cell Extraction Buffer PTR.

Example of human CD44 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human CD44 ELISA Kit (AB287168)

Interpolated concentrations of human CD44 in serum, plasma (citrate), plasma (EDTA), and plasma (heparin).

Interpolated concentration of native CD44 was measured in duplicate at different sample concentrations. Undiluted samples are 0.5% for serum, plasma (citrate), plasma (EDTA), and plasma(heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human CD44 ELISA Kit (AB287168)

Example of human CD44 standard curve in Sample Diluent NS.

Example of human CD44 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Serum, Cell Lysate, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 12.28 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human CD44 ELISA Kit ab287168 is a rapid single-wash 90-min Sandwich ELISA to measure Human CD44 in cell lysate, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 12.28 pg/mL.

How the assay works

Human CD44 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human CD44 ELISA Kit ab287168 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-CD44 antibody [EPR24092-8] - BSA and Azide free (Capture) ab276032
- Anti-CD44 antibody [EPR24092-126] - BSA and Azide free (Detector) ab276032

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.9" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.5" } ]

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Recovery

[ { "sample": "Serum", "range": "107 - 113 %", "average": "= 111" }, { "sample": "EDTA Plasma", "range": "99 - 108 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "104 - 114 %", "average": "= 107" }, { "sample": "Cell Lysate", "range": "103 - 117 %", "average": "= 112" }, { "sample": "Citrate plasma", "range": "100 - 120 %", "average": "= 112" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab287168-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB287168-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB287168-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB287168-CMP13", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB287168-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB287168-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB287168-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human CD44 Lyophilized Recombinant Protein", "number":"AB287168-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB287168-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD44 Capture Antibody", "number":"AB287168-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human CD44 Detector Antibody", "number":"AB287168-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB287168-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB287168-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

The protein expressed by gene CD44 functions as a cell-surface receptor involved in cell-cell interactions, cell adhesion, and migration, allowing cells to detect and react to changes in their tissue microenvironment. It is implicated in numerous cellular processes, including the activation, recirculation, and homing of T-lymphocytes, hematopoiesis, inflammation, and the response to bacterial infection. Through its ectodomain, CD44 interacts with extracellular matrix components like hyaluronan, collagen, growth factors, cytokines, and proteases, serving as a platform for signal transduction by forming protein complexes with receptor kinases and membrane proteases via its cytoplasmic domain. Effectors such as PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases, and phospholipase C coordinate signaling pathways that promote calcium mobilization and actin-mediated cytoskeleton reorganization crucial for cell migration and adhesion. This supplementary information is collated from multiple sources and compiled automatically.

See full target information CD44

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human CD44 ELISA Kit

ELISA - Human CD44 ELISA Kit (AB287168)

Sandwich ELISA - Human CD44 ELISA Kit (AB287168)

Sandwich ELISA - Human CD44 ELISA Kit (AB287168)

Sandwich ELISA - Human CD44 ELISA Kit (AB287168)

Sandwich ELISA - Human CD44 ELISA Kit (AB287168)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

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Primary Antibodies

Secondary Antibodies

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Product promise