AB45912

Human CD44 ELISA Kit

Name: Human CD44 ELISA Kit (ab45912) | Abcam
Brand: Abcam Limited
SKU: AB45912

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(8 Publications)

Human CD44 ELISA Kit is a sandwich ELISA designed to quantify Human CD44 with a sensitivity of 0.113 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 0.31 - 10 ng/mL

View Alternative Names

CD44, LHR, MDU2, MDU3, MIC4, CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, ECMR-III, PGP-1, PGP-I

2 Images

Sandwich ELISA - Human CD44 ELISA Kit (AB45912)

sELISA

Supplier Data

Sandwich ELISA - Human CD44 ELISA Kit (AB45912)

Representative Dose Response Curve (Logarithmic) using ab45912.

sELISA

Lab

Sandwich ELISA - Human CD44 ELISA Kit (AB45912)

huCD44 measured in biofluids showing quantity (ng) per mL of tested sample

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 0.113 ng/mL

Range

0.31 - 10 ng/mL

Assay time

2h 15m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human CD44 ELISA Kit ab45912 is a sandwich ELISA to measure Human CD44 in serum, plasma, cell culture supernatant with a sensitivity of 0.113 ng/ml.

How the assay works

A monoclonal antibody specific for CD44 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD44 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD44 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD44 present in the samples.

This kit will recognize both endogenous and recombinant Human CD44.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human CD44 ELISA Kit ab45912 protocol summary

1. Add standard or sample to each well used
2. Add prepared Biotinylated labeled detector antibody. Incubate at room temperature
3. Aspirate and wash each well
4. Add prepared Streptavidin-HRP mix to each well. Incubate at room temperature
5. Aspirate and wash each well.
6. Add the TMB Solution to each well until color develops and then add the Stop Solution
7. Immediately begin recording the color development

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 50, "mean": "9.95 ng/mL", "standardDeviation": "0.307", "coefficientOfVariability": "3.08" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 50, "mean": "1.07 ng/mL", "standardDeviation": "0.105", "coefficientOfVariability": "9.34" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 8, "mean": "9.85 ng/mL", "standardDeviation": "1.55", "coefficientOfVariability": "1.52" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 8, "mean": "10.5 ng/mL", "standardDeviation": "0.055", "coefficientOfVariability": "4.76" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab45912-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB45912-CMP03" }, { "size":"2 x 1 Vial", "name":"CD44 Standard", "number":"AB45912-CMP10" }, { "size":"2 x 1 Vial", "name":"CD44 Control", "number":"AB45912-CMP08" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB45912-CMP04" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB45912-CMP11" }, { "size":"1 x 7 mL", "name":"Biotinylated Antibody Diluent", "number":"AB45912-CMP01" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB45912-CMP02" }, { "size":"1 x 0.4 mL", "name":"Biotinylated anti-CD44 anitibody", "number":"AB45912-CMP12" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB45912-CMP06" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB45912-CMP05" }, { "size":"2 x 1 Unit", "name":"Plastic Plate Covers", "number":"AB45912-CMP07" }, { "size":"1 x 1 Unit", "name":"CD44 Microplate (12 x 8 well strips)", "number":"AB45912-CMP09" } ] }, "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab45912-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB45912-CMP03", "productcode":"" }, { "size":"4 x 1 Vial", "name":"CD44 Standard", "number":"AB45912-CMP10", "productcode":"" }, { "size":"4 x 1 Vial", "name":"CD44 Control", "number":"AB45912-CMP08", "productcode":"" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB45912-CMP11", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Plastic Plate Covers", "number":"AB45912-CMP07", "productcode":"" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB45912-CMP05", "productcode":"" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB45912-CMP02", "productcode":"" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB45912-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB45912-CMP06", "productcode":"" }, { "size":"2 x 1 Unit", "name":"CD44 Microplate (12 x 8 well strips)", "number":"AB45912-CMP09", "productcode":"" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB45912-CMP01", "productcode":"" }, { "size":"2 x 0.4 mL", "name":"Biotinylated anti-CD44 anitibody", "number":"AB45912-CMP12", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment (PubMed : 16541107, PubMed : 19703720, PubMed : 22726066). Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection (PubMed : 7528188). Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed : 18757307, PubMed : 23589287). Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion (PubMed : 15123640). Upon interaction with LGALS9 ligand, activates downstream signaling components including LCK, ERK and MAPK to promotes NK cell activation (PubMed : 37006235).. (Microbial infection) Promotes foot-and-mouth disease virus internalization via macropinocytosis.

See full target information CD44

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:645 PubMed36746966

2023

Establishment and validation of a plasma oncofetal chondroitin sulfated proteoglycan for pan-cancer detection.

Applications

Unspecified application

Species

Unspecified reactive species

Pei-Fen Zhang,Zi-Yi Wu,Wen-Bin Zhang,Yong-Qiao He,Kexin Chen,Tong-Min Wang,Haixin Li,Hong Zheng,Dan-Hua Li,Da-Wei Yang,Ting Zhou,Chang-Mi Deng,Ying Liao,Wen-Qiong Xue,Lian-Jing Cao,Xi-Zhao Li,Jiang-Bo Zhang,Si-Qi Dong,Fang Wang,Mei-Qi Zheng,Wen-Li Zhang,Jianbing Mu,Wei-Hua Jia

Journal of cellular and molecular medicine 26:2089-2103 PubMed35146909

2022

CD44/ERM/F-actin complex mediates targeted nuclear degranulation and excessive neutrophil extracellular trap formation during sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Yiming Shao,Linbin Li,Lu Liu,Yunxi Yang,Jiamin Huang,Dongdong Ji,Yuying Zhou,Yi Chen,Zhechen Zhu,Bingwei Sun

Zhongguo fei ai za zhi = Chinese journal of lung cancer 24:583-590 PubMed34187156

2021

[Expression of CD44 in Tumor Tissue and Serum of Small Cell Lung Cancer and Its Clinical Prognostic Significance].

Applications

Unspecified application

Species

Unspecified reactive species

Ying Wang,Yi Guo,Haifeng Lin,Lina Zhang,Hongmei Zhang,Qunhui Wang,Fanbin Hu,Jie Li,Baolan Li,Tongmei Zhang

Molecules (Basel, Switzerland) 26: PubMed33440679

2021

Zeaxanthin-Rich Extract from Superfood Selectively Modulates the Cellular Adhesion and MAPK Signaling in Melanoma versus Normal Skin Cells In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Diana Cenariu,Eva Fischer-Fodor,Adrian Bogdan Țigu,Andrea Bunea,Piroska Virág,Maria Perde-Schrepler,Vlad-Alexandru Toma,Andrei Mocan,Ioana Berindan-Neagoe,Adela Pintea,Gianina Crișan,Mihai Cenariu,Alma Maniu

Cancer research 74:2630-41 PubMed24618343

2014

CD44v8-10 is a cancer-specific marker for gastric cancer stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Min Lau,Eileen Teng,Hui Shan Chong,Kirsten Anne Pagaduan Lopez,Amy Yuh Ling Tay,Manuel Salto-Tellez,Asim Shabbir,Jimmy Bok Yan So,Shing Leng Chan

Cell death & disease 4:e613 PubMed23640455

2013

A caspase-3 'death-switch' in colorectal cancer cells for induced and synchronous tumor apoptosis in vitro and in vivo facilitates the development of minimally invasive cell death biomarkers.

Applications

ELISA

Species

Human

K L Simpson,C Cawthorne,C Zhou,C L Hodgkinson,M J Walker,F Trapani,M Kadirvel,G Brown,M J Dawson,M MacFarlane,K J Williams,A D Whetton,C Dive

PloS one 7:e37797 PubMed22629457

2012

CCL18 in a multiplex urine-based assay for the detection of bladder cancer.

Applications

ELISA

Species

Human

Virginia Urquidi,Jeongsoon Kim,Myron Chang,Yunfeng Dai,Charles J Rosser,Steve Goodison

PLoS computational biology 6: PubMed20885780

2010

Differentially expressed RNA from public microarray data identifies serum protein biomarkers for cross-organ transplant rejection and other conditions.

Applications

ELISA

Species

Human

Rong Chen,Tara K Sigdel,Li Li,Neeraja Kambham,Joel T Dudley,Szu-Chuan Hsieh,R Bryan Klassen,Amery Chen,Tuyen Caohuu,Alexander A Morgan,Hannah A Valantine,Kiran K Khush,Minnie M Sarwal,Atul J Butte

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human CD44 ELISA Kit

Sandwich ELISA - Human CD44 ELISA Kit (AB45912)

Sandwich ELISA - Human CD44 ELISA Kit (AB45912)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Assay Kits

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (8)

Product promise